Human laminin receptor crystal and uses thereof

ABSTRACT

A human laminin receptor crystal is disclosed. Methods are disclosed for using various computer and non-computer means in order to develop models for use in the development of novel therapeutics that block and/or mimic laminin receptor interactions in the setting of, among others, Alzheimer&#39;s disease, other neurological disorders, cancer, and viral and bacterial infections.

CROSS-REFERENCE TO RELATED APPLICATION

This application claims the benefit of and priority to U.S. Provisional Application Ser. No. 60/981,583, filed on Oct. 22, 2007, the entirety of this application hereby incorporated by reference.

STATEMENT REGARDING FEDERALLY SUPPORTED RESEARCH OR DEVELOPMENT

This invention was supported, in whole or in part, by U.S. Public Health Service grants CA100687 and CA68498 from the National Cancer Institute, National Institutes of Health, and Department of Health and Human Services. The Government has certain rights in the invention.

FIELD OF THE INVENTION

The present disclosure is directed to the crystal structure of a laminin receptor (hereinafter “LamR”), and furthermore is directed to developing novel therapeutics that block and/or mimic laminin receptor interactions in the setting of, among others, Alzheimer's Disease, other neurological disorders, cancers and viral and bacterial infections.

Throughout this Application, various publications are referenced by the lead author's name and date of publication. Full citations of these publications may be found in the References Cited. The disclosures of these publications are hereby incorporated by reference into this Application in, order to more fully describe the state of the art as of the date of the invention described and claimed herein.

BACKGROUND OF THE INVENTION

The LamR is a nonintegrin cell-surface protein that has been identified as the receptor for, among others, the extracellular matrix molecule laminin-1 (Rao 1983), pathogenic prion protein. (Gauczynski 2001), Sindbis virus (Wang 1992), Venezuelan equine encephalitis virus (VEE) (Ludwig 1996), cytotoxic necrotizing factor type I and II (McNichol 2007), and Adeno-associated virus serotypes 2, 3, 8 and 9 (Akache 2006). LamR binds to laminin with high affinity, mediating interactions between laminin and the extracellular environment that affect cell adhesion, tumor growth and metastasis.

Two types of LamR, the 67 kDa and the 37 kDa, are found on the surface of cells (Gauczynski 2001.). The 67 kDa laminin receptor may be formed by the dimerization of the 37 kDa laminin receptor. Phylogenetic analysis also suggests that the LamR is a ribosomal protein that acquired the additional novel function of the laminin receptor during evolution (Ardini 1998).

Structural characterization of the LamR may permit greater understanding of how the LamR interacts with its binding partners. This in turn may facilitate development of therapeutics that may block and/or mimic LamR interactions in the setting of, among others, AD, other neurological disorders, cancer, and viral and bacterial infections.

In addition, structural analysis of the LamR may provide insight into the development of therapeutics that aid in the prevention of various disease states, including tumor growth and metastasis.

Because of the role of LamR interaction, with laminin in normal and cancerous cells, as well as its role as a receptor for Sindbis virus, adeno-associated virus and pathogenic prion protein, it is desirable to study LamR using crystallographic methods.

SUMMARY OF THE INVENTION

The present disclosure provides a crystalline form of a human LamR, and provides methods for developing novel therapeutics that block and/or mimic LamR interactions in the setting of, among others, AD, other neurological disorders, cancer, and viral and bacterial infections.

According to aspects illustrated herein, a crystal is provided that includes at least a portion of a LamR. The term “at least, a portion” refers to a LamR having some or all residues of a LamR protein. In some aspects, the term “at least a portion” refers to a LamR having residues 1-220 of a full-length LamR.

According to aspects illustrated herein, a method is disclosed for identifying an agent that interacts with a LamR, that includes: providing at least a portion of the LamR as a crystal; and employing computational analysis to design or select an agent that interacts with the crystal.

According to further aspects illustrated herein, a method is disclosed for identifying an agent that interacts with a LamR that includes: providing at least a portion of the LamR as a crystal; generating a three-dimensional model of the crystal using a set of relative structural coordinates according to Appendix 1 with a root mean square deviation from residue backbone atoms being not more than 1.5 Å, the set of relative structural coordinates being based on the crystal; and employing the three-dimensional model to design or select an agent that interacts with the crystal.

According to further aspects illustrated herein, there is disclosed a method for identifying an activator or inhibitor of a molecule or molecular complex having an active binding site that includes: generating a three dimensional representation of the molecule or molecular complex including an active binding site using a set of relative structural coordinates of amino acid residues Leu25, Gly26, Gly27, Ile46, Arg80, Asp126, Arg128, Asn149, Asp151, Ser152, Asp164, Asp165, Lys166, Gln33, Gln35, Tyr36, Lys57, Ala137, Ser138, Asn141, Leu142, Pro143, Thr144, Met10, Leu19, His24, Ile49, Asn50, Leu51, Lys52, Gln9, Lys11, Trp55, Leu59, Glu181, Arg184, Met185 Arg191, Thr28, Ile46, Pro127, Ala129, Val15, Phe18, Leu25, Arg80 and Asn81, or any portion thereof, with a root mean square deviation from residue backbone atoms of the amino acid residues being not more than 1.5 Å; and selecting or designing a candidate activator or inhibitor by performing computer fitting analysis of the candidate activator or inhibitor with the three dimensional representation generated.

According to further aspects illustrated herein, there is disclosed a method for identifying a potential compound able to interact with a LamR family member that includes: selecting or designing the potential compound by performing rational drug design with a computer readable data storage material encoded with computer readable data including structure coordinates as listed in Appendix 1, wherein the selecting or designing is performed in conjunction with computer modeling; contacting the potential compound with at least a portion of a LamR; and detecting an ability of the potential compound to interact with the LamR family member.

According to yet further aspects illustrated herein, methods of identifying an agent that interacts with LamR, and/or identifying an activator or inhibitor of a molecule or molecular complex having an active binding site, and/or identifying a potential compound that is able to interact with a LamR family member may also be performed without the aid of a computer means. Indeed, the present invention includes manual and computational methods of determining and/or deriving the above, in accordance with a crystal of at least a portion of the LamR being provided.

According to even further aspects illustrated herein, there is disclosed a crystal that includes at least a portion of a p40 ribosomal protein, wherein the p40 ribosomal protein is in tetragonal space group P4₃2₁2 and has unit cell dimensions a=75.7 Å, b=75.7 Å, and c=99 Å.

Applicants have also found that LamR interacts with many ligands including, but not limited to, laminin, prions, Sindbis virus, and EGCG (the major polyphenol in green tea).

LamR may also be implicated in the onset of Alzheimer's Disease (hereinafter “AD”) and certain dancers.

With respect to cancer, it has been observed that one of the ways EGCG may mediate anticancer effects is through binding to the LamR. Applicants have found that the Sindbis vector targets overexpressed LamR on tumor cells, resulting in the reduction of tumor load. Thus, targeted therapeutic design with respect to the LamR's interaction with its other binding partners will be efficacious.

For example, Applicants have discovered that blocking the interaction of certain ligands with the LamR will prevent internalization of a pathogen. This blocking prevents the removal of LamR from the cell surface. Together, these actions may very well effect disease progression.

With respect to AD, laminin has been shown to interact with the amyloid beta 1-40 peptide (hereinafter “Aβ”), thus blocking fibril formation and even inducing depolymerization of pre-formed fibrils. LamR may be of therapeutic interest for controlling the amyloidosis that occurs with AD.

Targeted therapeutic design against LamR's interaction with its other binding partners may also be efficacious. Further study of the structure of the LamR crystal provided herein will contribute to an understanding of how LamR interacts with its binding partners and aid in the development of therapeutics that can block and/or mimic LamR interactions in the setting of, among others, AD, other neurological disorders, cancers and viral and bacterial infections.

In addition, study of the LamR crystal structure may also lead to discovery of co-crystals representing the crystal forms and structures of the LamR when interacting with binding partners, which may further aid in the prevention or treatment of the types conditions noted above.

BRIEF DESCRIPTION OF THE DRAWINGS

The patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.

The presently disclosed embodiments will be further explained with reference to the attached drawings, wherein like structures are referred to by like numerals throughout the several views. The drawings are not necessarily to scale, the emphasis having instead been generally placed upon illustrating the principles of the presently disclosed embodiments.

FIG. 1 shows the sequence homology between the 37LRP/p40 orthologs and the H. sapien LamR-220 of the presently disclosed embodiments. Orthologs are C. elegans SA ribosomal protein (p40), S. cerevisiae S2p ribosomal protein, and A. fulgidus S2p ribosomal protein. Residue numbering is for human LamR-220.

FIGS. 2A and 2B show the bioactivity of LamR-220 of the presently disclosed embodiments, in vitro. FIG. 2A shows the in vitro LamR-220 binding affinity for laminin-1. (LamR-220 is illustrated by a solid black line and LamR-295 is illustrated by a dashed black line. n=3±SEM.) LamR-220 K_(d)=1.7 μM. LamR-295 K_(d)=0.7 μM. FIG. 2B shows Sindbis viral vector infection inhibition by LamR-220 in baby hamster kidney (hereinafter “BHK”) cells, SV=Sindbis vector (MOI=100), LamR=recombinant LamR-220. n=2±SEM. Statistical significance was calculated by a two-tailed student t-test (P<0.05).

FIGS. 3A, 3B-1, 3B-2 and, 3C show the crystal structure of human LamR-220 of the presently disclosed embodiments. FIG. 3A shows a ribbon diagram of LamR-220 with α helices labeled “A”, “B”, “C”, “D” and “E” and β strands, labeled “1”, “2”, “3”, “4”, “5”, “6” and “7”. FIGS. 3B-1 and 3B-2 show the superimposition of LamR-220 and A. fulgidus S2p (1VI6). Regions of divergence between the two structures are at residues 111-118 and 188-196. FIG. 3C shows a LamR-220 dimer. Residues in the dimer interface are labeled. The crystallographic two-fold axis is vertical.

While the above-identified drawings set forth presently disclosed embodiments, other embodiments are also contemplated, as noted in the discussion.

DETAILED DESCRIPTION OF THE INVENTION

As noted above, the embodiments disclosed herein relate to the three dimensional crystal structure of a LamR and to developing novel therapeutics that may block and/or mimic LamR interactions in the setting of, among others, AD, other neurological disorders, cancer, and viral and bacterial infections.

Atomic structural coordinates for the LamR of the presently disclosed embodiments were derived from the analysis of high resolution X-ray diffraction patterns of crystals of a 220-residue version LamR (hereinafter sometimes referred to as “LamR-220”). These atomic structural coordinates are given in Appendix 1.

Definitions

All scientific terms are given their ordinary meanings as understood by those of skill in the art, unless an alternate meaning is set forth below.

As used herein, the term “LamR” refers to the laminin receptor.

As used herein, the term “p40” refers to the p40 ribosome-associated protein.

As used herein, the term “molecular replacement” means a method of solving crystal structure using a set of atomic coordinates of a structurally related molecule.

As used herein, the term “binding site” or “binding pocket” refers to a region of a protein or protein/RNA complex or RNA that binds or interacts with a particular compound.

As used herein, the term “structural coordinates” refers to mathematical Cartesian coordinates corresponding to an atom's spatial relationship to other atoms in a molecule or molecular complex. Structural coordinates may be obtained using x-ray crystallography techniques or NMR techniques, or may be derived from mathematical equations related to the X-ray diffraction patterns obtained by diffracting X-rays off a crystal. The diffraction data are used to calculate an electron density map of the unit cell comprising the crystal; said maps are used to establish the positions of the atoms (e.g., the structure coordinates) within the unit cell. Various software programs allow for the graphical representation of a set of structural coordinates to obtain a three dimensional representation of a molecule or molecular complex. Those of skill in the art understand that a set of structure coordinates determined by X-ray crystallography contains standard errors.

As used herein, the term “β sheet” refers to two or more polypeptide chains (or beta strands) that run alongside each other and are linked in a regular manner by hydrogen bonds between the main chain C═O and N—H groups. Therefore all hydrogen bonds in a beta-sheet are between different segments of polypeptide. Most β-sheets in proteins are all-parallel (protein interiors) or all-antiparallel (one side facing solvent, the other facing the hydrophobic core). Hydrogen bonds in antiparallel sheets are perpendicular to the chain direction and spaced evenly as pairs between strands. Hydrogen bonds in parallel sheets are slanted with respect to the chains direction and spaced evenly between strands.

As used herein, the term “α helix” refers to the most abundant helical conformation found in globular proteins. The average length of an α helix is 10 residues. In, an α helix, all amide protons point toward the N-terminus and all carbonyl oxygens point toward the C-terminus. The repeating nature of the phi/psi pairs ensure this orientation. Hydrogen bonds within an α helix also display a repeating pattern in which the backbone C═O of residue X (wherein X refers to any amino acid) hydrogen bonds to the backbone HN of residue X+4. The α helix is a coiled structure characterized by 3.6. residues per turn, and translating along, its axis 1.5 Å per amino acid. Thus the pitch is 3.6×1.5 or 5.4 Å. The screw sense of alpha helices is always right-handed.

As used herein, the term “loop” refers to any other conformation of amino acids (i.e. not a helix, strand or sheet). Additionally, a loop may contain bond interactions between amino acid side chains, but not in a repetitive, regular fashion.

Amino acid residues in peptides are abbreviated as follows: Phenylalanine is Phe or F; Leucine is Leu or L; Isoleucine is Ile or I; Methionine is Met or M; Valine is Val or V; Serine is Ser or S; Proline is Pro or P; Threonine is Thr or T; Alanine is Ala or A; Tyrosine is Tyr or Y; Histidine is His or H; Glutamine is Gln or Q; Asparagine is Asn or N; Lysine is Lys or K; Aspartic Acid is Asp or D; Glutamic Acid is Glu or E; Cysteine is Cys or C; Tryptophan is Trp or W; Arginine is Arg or R; and Glycine is Gly or G.

As used herein, the term “root mean square deviation” is the square root of the arithmetic mean of the squares of the deviations from the mean, and is a way of expressing deviation or variation from the structural coordinates described herein. The present disclosure includes all embodiments comprising conservative substitutions of the noted amino acid residues resulting in same structural coordinates within the stated root mean square deviation.

As used herein, an “active site” refers to a region of a molecule or molecular complex that, as a result of its shape and charge potential, favorably interacts or associates with another agent (including, without limitation, a protein, polypeptide, peptide, nucleic acid, including DNA or RNA, molecule, compound or drug) via various covalent and/or non-covalent binding forces. An active site in accordance with the present disclosure may include, for example, the actual site of substrate binding with laminin receptor, as well as accessory binding sites adjacent or proximal to the actual binding site of substrate binding that nonetheless may affect laminin receptor activity upon interaction or association with a particular agent, either by direct interference with the actual site of substrate binding or by indirectly affecting the steric conformation or charge potential of the laminin receptor and thereby preventing or reducing binding of substrate to the laminin receptor at the actual site of substrate binding.

As used herein, an “agent” includes a protein, polypeptide, peptide, nucleic acid (including DNA or RNA), molecule, compound or drug.

As used herein, the terms “a target structural motif” or “target motif” refer to any rationally selected sequence or combination of sequences in which the sequence(s) are chosen based on a three-dimensional configuration or electron density map which is formed upon the folding of the target motif. There are a variety of target motifs known in the art. Protein target motifs include, but are not limited to, enzymatic active sites, structural subdomains, epitopes, functional domains and signal sequences.

Structural similarity may be inferred from, e.g., sequence similarity, which can be determined by one of ordinary skill through visual inspection and comparison of the sequences, or through the use of well-known alignment software programs such as CLUSTAL (Wilbur, W. J. and Lipman, D. J. Proc. Natl. Acad. Sci. USA, 80, 726 730 (1983)) or CLUSTAL W (Thompson, J. D., Higgins, D. G. and Gibson, T. J., CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice, Nucleic Acids Research, 22:4.673 4680 (1994)) or BLAST® (Altschul S F, Gish W, et al., J. Mol. Biol., October 5; 215(3):403 10 (1990)), a set of similarity search programs designed to explore all of the available sequence databases regardless of whether the query is protein or DNA. A residue within a first protein or nucleic acid sequence corresponds to a residue within a second protein, or nucleic acid sequence if the two residues occupy the same position when the first and second sequences are aligned.

LamR Binding Domains and Experimental Details

The region of LamR responsible for binding laminin-1 is between amino acid residues 161 and 180, and was found using a synthetic peptide called peptide G which corresponds to those amino acids. Specifically, the domain within laminin-1, the Tyr-Ile-Gly-Ser-Arg (YIGSR) peptide, is responsible for binding with LamR. In additions peptide G treatment of cells has been shown to increase and stabilize laminin binding on tumor cells. Peptide. G has also been shown to bind heparin sulfate proteoglycans (hereinafter “HSPG”) and it has been suggested that laminin-1 interactions with LamR may also be mediated by HSPG binding.

Another synthetic peptide comprised of corresponding amino acid residues 205-229 of the LamR, has been shown to interact with both laminin-1 and heparin sulfate. This indicates that two sites play a role in LamR interactions with laminin. The interaction domains between LamR and prion protein have also been characterized. Yeast two-hybrid system and cell-binding assays suggest that LamR, contains a primary prion protein-binding region between amino acids 144 and 179 and a secondary indirect binding site that is HSPG-dependent between amino acids 161 and 179.

A potential region for interaction between LamR and Sindbis virus E2 has been identified to lie within the C-terminus of LamR. In fact, Wang et al. determined that binding of monoclonal antibody 1C3 (MAb 1C3) to LamR in culture cells blocked Sindbis virus attachment (Wang 1992). MAb1C3 is a monoclonal antibody that is reactive with an epitope within the C-terminal 48 amino acids (residues 248 to 295) of the LamR. This indicates that Sindbis virus also binds somewhere in this C-terminal domain of LamR.

According to Applicants and others, these data also implicate LamR as the major cell-surface receptor for Sindbis virus in vertebrate cells, but the specific interaction domain between Sindbis virus E2 was heretofore unclear. Thus, X-ray crystallographic studies of the LamR are critical to understanding the precise structure of the LamR and to characterizing the LamR binding interaction with Sindbis virus, as well and other key binding partners and agents.

Discussion

In accordance with the present disclosure, a crystal form of the LamR has been produced. The LamR crystal has structure: and properties described in further detail below.

Thus, the presently disclosed embodiments describe the resolution of a three dimensional crystal structure of a 220-residue version of human LamR precursor protein (hereinafter “LamR-220”). LamR-220 binds laminin-1 in vitro, as shown in FIG. 2A, and inhibits Sindbis virus vector infection of BHK cells, as shown in FIG. 2B. These data demonstrate that the first 220 residues of LamR are sufficient for interacting with key binding partners, including Sindbis virus.

Crystals of LamR-220 were obtained at a pH 6.0 and belong to the tetragonal space group P4₃2₁2 with one monomer in the asymmetric unit. The crystal structure of LamR-220 was determined by molecular replacement, using the crystal structure of the 30S ribosomal protein. S2p from Archaeoglobus fulgidus (PDB code 1VI6) (Badger 2005). Data collection and refinement statistics at 2.15 Å resolution are given in Appendix 1 and Table 1 below.

TABLE 1 LamR220 Data collectio n Space group P4₃2₁2 Cell dimensions a, b, c (Å) 75.66, 75.66, 98.965 a, b, g (°) 90.00, 90.00, 90.00 Resolution (Å)  50.0 (2.15)* R_(sym) or R_(merge) (%) 14.4 (47.7) I/sI 5.0 (4.0) Completeness (%) 100 (100) Redundancy 9.4 (9.7) Refinement Resolution (Å) 30.0-2.15 No. reflections 14762 R_(work)/R_(free) (%) 18.8/24.1 No. atoms 1727 Protein 1563 Ligand/ion 0 Water 164 Average B-factor 22.0 R.m.s. deviations Bond lengths (Å) 0.009 Bond angles (°) 1.17 *Values in parentheses are for highest-resolution shell (2.25-2.15 Å). One crystal was used for data collection.

Consistent with a sequence identity of 32% between the LamR and A. fulgidus S2p (residues 15-183 of FIG. 1), the two proteins share a similar overall architecture, classified (SCOP) as an α/β protein with a flavodoxin-like fold (see FIG. 3A). A central β sheet composed of five parallel β strands (β3-β7) is flanked by three α helices on one side (αB, αC and αE) and a single α helix (αD) on the other side. An N-terminal α helix (αA) and two anti-parallel β strands (β1-β2) pack against the α/β core of the protein. Residues 1-8 and 206-220 of LamR-220 are disordered in the structure.

FIGS. 3B-1 and 3B-2 show that the superimposition of the structures of LamR-220 and A. fulgidus S2p yields a root-mean-square deviation in Cα positions of just 0.9 Å (174 atoms) and reveals two areas in which the structures are divergent. The structures diverge at a segment between β4 and β5 (residues 111-118 in LamR) and a segment after the last α helix (αE) (residues 188-196 in LamR) in which LamR contains a five-residue insertion relative to A. fulgidis S2p. The segment between β4 and β5, contains an equal number of residues, in the two proteins. In A. fulgidis S2p, the segment is stabilized in a folded-back conformation via a salt bridge between Arg113 in this segment (Arg117 in LamR) and Asp93 (β4), the latter of which is not conserved in LamR, (Thr97). As shown in FIG. 3C, in the LamR-220 structure, this segment instead projects away from, the domain and packs against the same segment in a symmetry-related (two-fold) molecule. In this crystallographic dimer, Ala114 packs into a tight pocket in the symmetry related molecule formed by the β4-β5 segment and the end of αD, and Phe116 is in van der Waals contact with Tyr139 (αD). While Phe116 is generally conserved from S. cerevisiae through vertebrate species, Ala114 is conserved only in vertebrates. The total surface area buried in this interface is a modest 832 Å² and LamR-220 runs as a monomer in solution but, in the context of a membrane attachment and a possible covalent dimerization linkage (Landowski 1995), this crystallographic dimer could be functionally significant.

The structural differences noted between LamR-220 and A. fulgidis S2p could be important for ribosomal protein function or the acquired function as the receptor for laminin. Analysis of the 3.0 Å-resolution structure of the 30S ribosomal subunit from Thermus thermophilus (PDB code 1J5E) (Wimberly 2000) indicates that the two major structural deviations between human LamR-220 and A. fulgidis S2p (between β4 and β5 and after αE) would not appear to affect ribosomal function, since no RNA or protein contacts are present in these regions. This suggests that the structural differences in human LamR versus A. fulgidis S2p are important for laminin binding.

Previously, peptide segments of LamR, utilized in binding assays implicated a segment, residues 161-180, known as peptide G, as a binding epitope for laminin. (Castronovo 1991). In the LamR-220 crystal structure of the presently disclosed embodiments, this stretch of residues comprises the linker between β7 and most of αE. The only portion of this sequence that, is solvent accessible are residues 165-169 in the β7-αE linker. Without being limited to any particular theory, it is conceivable that the C-terminal tail of LamR-220 undergoes a conformational change, exposing residues within this segment. It is desirable to determine if other peptide segments of LamR are possible for use in binding assays as a binding epitope for laminin. Previously, a short putative transmembrane segment, residues 86-102 was suggested as a binding epitope for laminin (Castronovo 1991). However, analysis of the LamR-220 of the presently disclosed embodiments shows that this segment, encompassing β4 and most of αC, is an integral part of the protein fold and is unlikely to serve as a transmembrane helix.

In addition, the presently disclosed embodiments permit the use of molecular design techniques (whether utilizing a computer or not) to design, identify and synthesize chemical entities and compounds, including inhibitory compounds, capable of binding anywhere on the LamR and/or LamR-related proteins. As such, the structure of the disclosed LamR crystal may be used to identify potential LamR ligands.

Appendix 1 shows the atomic coordinates/x-ray diffraction data of the LamR-220 of the presently disclosed embodiments. The atomic coordinates/x-ray diffraction data may be used to create a physical three-dimensional model which can then be used to design molecular models of compounds that interact and/or bind with LamR.

Alternatively, the atomic coordinates/x-ray diffraction data may be represented as atomic model output data on computer readable media which is then used in a computer modeling system to calculate different molecules expected to interact and/or bind with the LamR. For example, computer analysis of the data permits calculation of the three-dimensional interaction of the LamR and a ligand to confirm that the ligand binds to, or changes the conformation of, particular domain(s) or subdomain(s) of the LamR. Ligands identified from the analysis of the physical or computer model are then synthesized and tested for biological activity with an appropriate screen.

Although various computer programs are described herein which may be used in conjunction with the present invention, one of skill in the art could of course perform all of the analysis described herein using manual computational methods after having been provided the crystal structure of the LamR. Thus, the practice of the present invention is not limited to using only computer means, but includes and encompasses all forms of computational methods (computers or otherwise).

When using a computer means, however, the atomic coordinates/x-ray diffraction data of the presently disclosed embodiments are generally provided on computer readable media. A skilled artisan is able to access the data and analyze it for structure determination and/or rational ligand (e.g., inhibitor) design using a computer based system.

A typical computer system includes hardware means, software means, and data storage means. The hardware means typically includes a central processing unit (CPU), input means, output means and data storage means. One skilled in the art will readily appreciate which of the currently available computer-based systems are suitable for use in the practice of the presently disclosed embodiments.

As has been disclosed in U.S. patent application Ser. No. 10/885,190, a variety of commercially available software programs are available for conducting the analysis and comparison of data in the computer-based, system. One skilled in the art will readily recognize which of the available algorithms or implementing software packages for conducting computer analyses can be utilized or adapted for use in the computer-based system. A variety of structural formats for the input and output means can be used to input and output the information in the computer-based systems of the presently disclosed embodiments.

As has also been disclosed, in U.S. patent application Ser. No. 10/885,190, a variety of comparing means can be used to compare a target sequence or target motif with the data storage means to identify structural motifs or interpret electron density maps derived in part from the atomic coordinates/x-ray diffraction data. One skilled in the art can readily recognize any one of the publicly available computer modeling programs that can be used.

As has been further disclosed in U.S. patent application Ser. No. 10/885,190, suitable software that can be used to view, analyze, design, and/or model a protein include Alchemy™, LabVision™, Sybyl™, Molcadd™, Leapfrog™, Matchmaker™, Genefold™ and Sitel™ (available from Tripos Inc., St. Louis, Mo.); Quanta™, Cerius2™, X-Plor™, CNS™, Catalyst™, Modeller™, ChemX™, Ludi™, Insight™, Discover™, Cameleon™ and Iditis™ (available from Accelrys Inc., Princeton, N.J.); Rasmol™ (available from Glaxo Research and Development; Greenford, Middlesex, U.K.); MOE™ (available from Chemical Computing Group, Montreal, Quebec, Canada); Maestro™ (available from Schrodinger Inc.); Midas/MidasPlus™ (available from UCSF, San Francisco, Calif.); VRML (webviewer—freeware on, the internet); Chime (MDL—freeware on the internet); MOIL, (available from University of Illinois, Urbana-Champaign, Ill.); MacroModel™ and GRASP™ (available: from Columbia University, New York, N.Y.); Ribbon™ (available from University of Alabama, Tuscaloosa, Ala.); NAOMI™ (available from Oxford University, Oxford, UK); Explorer Eyechem™ (available from Silicon Graphics Inc., Mountain View, Calif.); Univision™ (available from Cray Research Inc., Seattle Wash.); Molscript™ and O (available from Uppsala University, Uppsala, Sweden); Chem 3D™ and Protein Expert™ (available from Cambridge Scientific); Chain™ (available from Baylor College of Medicine, Houston, Tex.); Spartan™, MacSpartan™ and Titan™ (available from Wavefunction Inc., Irvine, Calif.); VMD™ (available from U. Illinois/Beckman Institute); Sculpt™ (available from Interactive Simulations, Inc., Portland, Oreg.); Procheck™ (available from Brookhaven National Laboratory, Upton, N.Y.); DGEOM (available from QCPE—Quantum Chemistry Program Exchange, Indiana University Bloomington, Ind.); RE_VIEW (available from Brunel University, London, UK); Xmol (available from Minnesota Supercomputing Center, University of Minnesota, Minneapolis, Minn.); Hyperchem™ (Available from Hypercube, Inc., Gainesville, Fla.); MDM Display (available from University of Washington, Seattle, Wash.); PKB (available from National Center for Biotechnblogy Information, NIH, Bethesda, Md.); Molecular Discovery Programmes (available from Molecular Discovery Limited, Mayfair, London); Grownol™ (available from Thistlesoft, Morris Township, N.J.); MICE (available from the San Diego Supercomputer Center, La Jolla, Calif.); Yummie and MCPro (available from Yale University, New Haven, Conn.); and upgraded versions thereof.

Another example of the type of software that a skilled artisan may use with the crystal structure of the LamR is software developed by Molsoft Technologies. Specifically, ICM Pro, ICM Browser Pro, ICM Homology and ICM VLS (hereinafter, collectively, “ICM”) together provide a solution for the viewing, analysis, modeling, design, and bonding simulation of proteins. ICM enables the analysis of a protein structure including, but not limited to, flagging problem regions, superimposing multiple structures, drug binding pocket and protein-protein interaction prediction, analysis of protein-ligand interactions, protein flexibility, distances and electrostatic properties. ICM also allows the full evaluation of the underlying crystallographic information contained within a Protein Databank file. One such function exhibits the full bio-molecular unit to see if crystal-crystal contacts have influenced the crystal structure. ICM's crystallographic capabilities include, but are not limited to: displaying the crystallographic cell, generating crystallographic neighbors, constructing biological units and applying transformations, and contouring and converting the electron density map to a grid energy map. ICM provides access to the chemical information and tools for accurate individual ligand-protein docking, peptide-protein docking and protein-protein docking, enabling rapid and accurate docking simulations. These tools provide scripting for small scale flexible ligand docking, procedures for protein-protein, and flexible peptide-receptor docking, and refinement of docking solutions in full atom representation. It also allows for the browsing of docking solutions, binding site analysis, visualization of grid potentials, adjustment of grid potential areas, and configurable preferences for ligand size and score thresholds. In application, ICM indexes and converts any chemical database to 3D, then docks and scores all molecules by estimated binding affinity. It separates binders and non-binders, and eliminates at least 99% of compounds that do not fit the protein or ligand pocket, and do not require experimental testing. One skilled in the art will appreciate that ICM (or similar software) may be used in order to determine what compounds can readily interact with the LamR.

Although the present invention is not limited to solely utilizing computers, computer simulation techniques may be advantageously used in connection with the. LamR crystal structure. In particular, the crystal structure of the LamR provides a means of discovering the characteristics and affinities of individual binding sites on the LamR. For example, EGCG is one such high-affinity compound whose specific binding sites with the LamR may be determined. Thus, the presently disclosed embodiments provide a means of accurately testing the effects of many molecular compounds on a given binding site which, in turn, may be used for the discovery and development of therapies for, but not limited to, cancer and AD. In addition, computer and/or other computational models may also be used for the discovery of inhibitors of LamR activity identified or designed by the methods of the presently disclosed embodiments. Once a potential ligand is identified from the analysis described above, the ligand can then be synthesized and tested for biological activity using an appropriate screening method discussed above and claimed below.

Analysis of the LamR crystal structure may also provide insights that aid in the development of novel therapeutics. In cancer, for example, the specific inhibition of the LamR function at the cell surface of tumor cells—either by binding of the catechin EGCG, which most likely competes with endogenous laminin, or by infection with Sindbis virus vector, which is internalized by receptor-mediated endocytosis—has been associated with anti-tumor effects (Tanaka 2000; Tachibana 2004; Tseng 2004; Cao 1999). Thus, both EGCG and Sindbis virus vectors, through two different mechanisms, reduce the ability of the LamR at the surface to interact with laminin.

Cancer cells invade the basement membranes through a process involving the binding of cell surface receptors like the LamR to laminin. While the presence of cancer invariably yields the overexpression of LamR, the expression of LamR has been particularly identified as correlating directly with increased invasiveness and the metastatic potential of tumors (Menard 1998). However, peptide G, for instance, has been identified as interfering with the binding of the LamR to laminin and therefore as effective in inhibiting tumor cell invasion. Similarly, as noted above, EGCG is well known to bind with the LamR, thereby precluding other binding from occurring. Study of the crystal structure of the LamR may permit precise determination of how these bindings occur.

Likewise, the crystal LamR may be used to further understand the role of the LamR in AD. With respect to AD, as previously noted, laminin has been shown to interact with the Aβ, thus blocking fibril formation and even inducing depolymerization of pre-formed fibrils. LamR may also be of therapeutic interest for controlling the amyloidosis that occurs with AD.

By way of a further background, AD is a progressive neurodegenerative disease, characterized by an early loss of memory as well as impairment of other intellectual functions, eventually resulting in the onset of dementia. The LamR may play a role in the progression of AD. Accordingly, study of the LamR crystal may play a role in treating and preventing AD.

The brains of humans afflicted with AD experience extensive neuronal loss in the hipocampus, neocortex, and other areas of the brain. Such brains have been found to be histopathologically characterized by numerous extracellular deposits, or lesions, comprising senile plaques and neurofibrillary tangles.

The interaction between laminin and the amyloid precursor protein (hereinafter “APP”)—which yields Aβ as a proteolytic fragment of APP—is, generally linked to the progressive development of the senile plaques (also known as amyloid plaques) which appear to disrupt the extracellular matrix when APP is overproduced. Aβ polymerizes into amyloid fibrils and is the major component of the plaques. These lesions have neurotoxic effects and are one of the causes, if not the primary cause, of neurodegeneration. AD brain tissue also has elevated expression of laminin compared to normal brain tissue.

Nonetheless, laminin has been found to be a potent inhibitor of the Aβ fibril formation responsible for the development of the plaques, as laminin has been shown to attenuate the neurotoxicity of amyloid. A chain containing the sequence IKVAV (a laminin-derived peptide), for example, has been found to inhibit amyloidogenesis by depolymerizing Aβ. This inhibition appears to be due to laminin's particular interaction with Aβ which results in the inducement of depolymerization of Aβ fibrils that are so instrumental in the onset and generation of AD and that are present in its telltale lesions. Laminin has also been shown to modulate the biogenesis of APP. Such interactions have also inhibited the toxic effects on rat primary hippocampal neurons.

Screening mechanisms as disclosed herein may also be used for the discovery and design of specific therapeutic drugs which treat various disease states by targeting the LamR. Once a drug is selected, the drug may then be produced through ordinary chemical synthesis. In addition, a pharmaceutically acceptable carrier may then be added thereto.

Accordingly, a method for producing a pharmaceutical composition which comprises a step of producing the drug obtainable by the above mentioned screening methods is within the scope of the presently disclosed embodiments.

As further disclosure of the presently disclosed embodiments, the, following Preparations and Examples are provided to illustrate specific embodiments and aspects of the presently disclosed embodiments. The illustration of specific embodiments and aspects, however, is not intended to limit the scope of this disclosure.

Materials And Methods

(i) Recombinant LamR Expression and Purification

Full-length human laminin receptor having residues 1-295 (LamR-295) was cloned into an E. coli expression vector that includes a TEV-cleavable, N-terminal 6×His-tag. The vector encoding LamR-295 was transformed into E. coli strain BL21 (DE3*) and cultures were grown in Luria broth media at 37° C. to an OD₆₀₀ of 0.6. Protein expression was induced by the addition of isopropyl-thiogalactopyranoside (IPTG, 0.1 mM) for 12 hours at 20° C. Cells were harvested, resuspended in lysis buffer (50 mM Tris (pH 8.0), 300 mM NaCl, 0.1% Triton X-100, 10% glycerol, EDTA-free protease inhibitor tablet (Roche)) and lysed by French press. The lysate was centrifuged at 16,000 RPM for 30 minutes and the supernatant was collected. The soluble fraction was purified by Ni-NTA chromatography (Qiagen), followed by gel filtration chromatography (Superdex 75, Amersham). Protein was concentrated in spin concentrators (Amicon, Millipore).

Expression and purification of LamR-295 was verified by coomassie staining and Western blot using both anti-His and anti-LamR antibodies. Purification of LamR-295 resulted in an impure protein product which was evident by multiple bands in both the coomassie stain and Western blot. In order to crystallize a protein, an extremely high level of purity is necessary. Thus, various shorter-length LamRs were subcloned from the full-length human LamR-295 cDNA and expression and purification was tested.

Next, the following LamR constructs were tested for expression and purification for crystallography purposes: residues 1-198, 12-198, 1-220, 12-220, and 1-206. Constructs comprising residues 12-198 and 1-198 were tested due to a sequence alignment between T. thermophilus 40S ribosomal protein S0-A, which was solved by CryoEM structure. Residues 12-220 and 1-220 were tested because trypsin digestion of the full-length LamR construct corresponded to a stable protein of 25 kDa, which approximately corresponds to amino acids 1-220. In addition, N-terminal sequencing of LamR-295 confirmed that the full-length construct was not being degraded from the N-terminus.

Due to the highly pure protein product (>95%) obtained from the LamR-220 construct, to the LamR-220 construct was chosen for pursuing crystallization screenings Residues 1-220 of human 37 kDa LamR protein (LamR-220,) were subcloned from a full-length LamR cDNA into an E. coli expression vector that includes a TEV-cleavable, N-terminal 6×His-tag. The construct was verified by automated DNA sequencing. The vector encoding LamR-220 was transformed into E. coli strain BL21 (DE3*), and cultures were grown in Luria broth media at 37° C. to an OD₆₀₀ of 0.6. Protein expression was induced by the addition of isopropyl-thiogalactopyranoside (IPTG, 0.1 mM) for 12 hours at 20° C. Cells were harvested, resuspended in lysis buffer (50 mM Tris (pH 8.0), 300 mM NaCl, 0.1% Triton X-100, 10% glycerol, EDTA-free protease inhibitor tablet (Roche)) and lysed by French press. The lysate was centrifuged at 16,000 RPM for 30 minutes and the supernatant was collected. The soluble fraction was purified by Ni-NTA chromatography (Qiagen), followed by gel filtration chromatography (Superdex 75, Amersham). Protein was concentrated in spin concentrators (Amicon, Millipore).

(ii) Crystallization and Data Collection

The most frequently used crystallization method is a vapor diffusion technique in which a 1 μl droplet of recombinant purified protein is mixed with an equal volume of the crystallizing solution that contains buffer, salt and precipitant. This droplet is placed on a siliconized glass cover slip which is inverted and sealed into place over a reservoir of 500 μl of the crystallization solution. The concentration difference between the drop and the reservoir drives the system to equilibrium by diffusion through the vapor phase.

Crystals of LamR-220 were grown at 17° C. by the hanging-drop vapor diffusion method in drops containing a 1:1 (v/v) ratio of protein solution at 10 mg/ml and reservoir solution containing 17% (w/v) PEG, 10 K, 120 mM sodium citrate, 100 mM MES [pH 6.0], and 5% (w/v) PEG 1.5 K. Crystals belong to the tetragonal space group P4₃2₁2 with unit cell dimensions of a=75.666 Å, b=75.666 Å, and c=98.965 Å. Crystals were soaked briefly in crystallization buffer with 20% glycerol (v/v) and then flash frozen in liquid nitrogen. There is one LamR-220 molecule in the asymmetric unit with a solvent content of 56%. Diffraction data were collected to a resolution of 2.15 Å at X29 at the National Synchrotron Light Source at Brookhaven National Laboratory. Data were processed with the HKL2000 (Otwinowski 1997). A molecular replacement solution was found with AMoRE (Navaza 1994) using as a search model the structure of the A. fulgidus 30s ribosomal protein S2p [PDB code 1VI6, chain A] (Badger 2005). Rigid-body refinement, simulated annealing and positional and B-factor refinement were performed with CNS (Brunger 1998) and Refmac (Mushudov 1997). Coot was used for model building (Emsley 2004). According to PROCHECK (Laskowski 1993), 94.8% of the residues have backbone torsion angles in most favored regions and 5.2% in additional allowed regions.

(iii) In vitro Binding Affinity for Laminin-1

LamR-220 and LamR-295 were tested in vitro for their ability to bind with laminin-1. White polystyrene ELISA 96-well microplates, precoated with murine laminin-1 (NEBiolabs), were blocked O/N at 4° C. with blocking buffer (3.0% BSA, 0.1% sodium azide in PBS). Wells were incubated with increasing concentrations of LamR-220 or LamR-295, which was Ni-affinity purified as described, above for 1 hour at 37° C. Each well was washed three times with blocking buffer. Penta-His HRP conjugate (1:500) (Qiagen) was incubated for 2 hours at RT and wells were washed three times with blocking buffer. Substrate solution was added and incubated for approximately 15 minutes before reading fluorescent absorbance was detected at 490 nm on an ELISA plate reader (EL_(x)800, Biotek Instruments, Inc.). Controls for buffer alone and nonspecific protein, BSA (NEBiolabs), were also tested. Experiments were performed in triplicate. A binding curve and K_(d) was generated for LamR-220 and LamR-295. The K_(d) was calculated using a one-site binding hyperbola and the equation Y=B_(max)×X/(k_(d)+X). Each group was tested in triplicate and binding affinity was determined by normalizing to background fluorescence. Results are shown in FIG. 2A.

(iv) Protocol for Protein Purification

A protocol for protein purification includes, but is not limited to, the following:

Expression:

-   -   1. inoculate 100 ml LB+30 μg/ml Kanymycin O/N at 37° C.     -   2. increase volume to 800-1000 mL LB+Kan in 2 L flask     -   3. grow at 37° C. until OD₆₀₀=0.4 to 0.6     -   4. induce with 1 mM IPTG O/N at 20° C.     -   5. spin down at 10,000 rpm for about 30 minutes and store         pellets at −80° C. or Ni-purify

Nickel-Affinity Purification:

-   -   1. resuspend 1 L pellet in 30 mL of lysis buffer with 1 tablet         EDTA-free protease inhibitor (do this in ice bucket)         -   a. dissolve protease tablet in lysis buffer before adding to             protein pellet     -   2. sonicate for about 20 minutes (3 seconds on, 12 seconds off,         at level 3)         -   a. in ice bucket         -   b. OR French press two times     -   3. spin down at 16,000 rpm for 30-45 minutes     -   4. filter supernatant in 0.45 μm Millipore 150 ml sterile         filters     -   5. add 1 mL Ni-agarose beads/1 L original inoculation culture to         large 20 mL disposable column         -   a. add stop cock to control flow     -   6. add 10 mL lysis buffer over beads and let flow through to         wash     -   7. add protein supernatant, collect flow through (FT)         -   a. control flow so that it is fairly slow     -   8. add 20 mL wash buffer and collect (W1)     -   9. add another 20 mL wash buffer and collect (W2)     -   10. add 5 mL elution buffer and, collect five 1 mL fractions         (E1-5)     -   11. run 10 μL of PC, FT, W1, W2, E1-5 in 15% SDS-PAGE     -   12. stain with 0.025% coomassie stain for 15-30 minutes, destain

Size Exclusion Chromatography:

-   -   1. collect fractions with most protein and concentrate to 2 mL,         filter     -   2. wash Superdex-75 column with S75 buffer     -   3. run protein on Superdex-75 column and collect fractions     -   4. run fractions in 15% SDS-PAGE     -   5. concentrate to desired concentration

(v) In vitro Sindbis Virus Vector Inhibition

Three million (3×10⁶) BHK-55 cells Were plated in a 12-well plate for 24 hours in αMEM (Cellgro) supplemented with 10% FBS. Sindbis virus vector that expressed a luciferin reporter upon replication (multiplicity of infection (MOI)=100) was incubated with control protein (BSA, NEBiolabs), LamR-220, or alone and rotated at room temperature for 1 hour. Cells were washed with Optimem (Gibco) and 300 μl of sample was added. Samples were incubated rotating gently for 1 hour at room temperature to allow Sindbis viral vector attachment. The supernatant was aspirated, cells were washed with PBS, and 1 ml of media was added. The cells were incubated O/N at 37° C. and media was aspirated the next day. 200 μl of unsupplemented media and 200 μl of luciferin substrate (SteadyGlo Luciferase Assay, Promega) was added. The cells were shaken vigorously for 15 minutes. The relative luciferase units (RLU), which correspond to infectivity of Sindbis virus vector, of each sample were read using a luminometer (Glomax 20/20, Promega). Experiments were performed in triplicate. A two-tailed student t-test was performed, (P<0.05) to determine statistical significance. Results are shown in FIG. 2B.

All patents, patent applications, and published references cited herein are hereby incorporated by reference in their, entirety. This disclosure presents illustrative embodiments by way of representation and not limitation. Numerous other modifications and embodiments may be devised by those skilled in the art which fall within the scope and spirit of the principles of the presently disclosed embodiments and the following claims.

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APPENDIX 1 X-RAY DATA COORDINATES FOR LRP-220 (SEQ ID NO: 5) Unit cell: 75.666  75.666  98.965  90.00  90.00  90.00 Space Group: P4₃2₁2 ATOM 1 N GLN X 9 32.089 42.564 44.187 1.00 39.73 N ATOM 2 CA GLN X 9 32.301 44.031 44.338 1.00 39.55 C ATOM 3 CB GLN X 9 32.131 44.729 42.990 1.00 39.97 C ATOM 4 CG GLN X 9 32.703 46.131 42.948 1.00 41.74 C ATOM 5 CD GLN X 9 31.798 47.087 42.215 1.00 43.97 C ATOM 6 OE1 GLN X 9 30.698 46.713 41.797 1.00 45.56 O ATOM 7 NE2 GLN X 9 32.244 48.335 42.062 1.00 44.38 N ATOM 8 C GLN X 9 31.341 44.621 45.376 1.00 38.74 C ATOM 9 O GLN X 9 31.550 45.728 45.883 1.00 38.77 O ATOM 10 N MET X 10 30.285 43.872 45.679 1.00 37.79 N ATOM 11 CA MET X 10 29.419 44.176 46.807 1.00 36.89 C ATOM 12 CB MET X 10 28.177 43.277 46.769 1.00 37.05 C ATOM 13 CG MET X 10 27.190 43.495 47.905 1.00 36.49 C ATOM 14 SD MET X 10 25.593 42.715 47.601 1.00 37.00 S ATOM 15 CE MET X 10 26.002 40.969 47.535 1.00 36.22 C ATOM 16 C MET X 10 30.222 43.953 48.090 1.00 36.08 C ATOM 17 O MET X 10 30.799 42.881 48.285 1.00 35.72 O ATOM 18 N LYS X 11 30.270 44.968 48.947 1.00 35.03 N ATOM 19 CA LYS X 11 31.070 44.896 50.163 1.00 34.37 C ATOM 20 CB LYS X 11 31.573 46.284 50.571 1.00 34.63 C ATOM 21 CG LYS X 11 32.737 46.797 49.708 1.00 34.83 C ATOM 22 CD LYS X 11 33.164 48.201 50.118 1.00 35.43 C ATOM 23 CE LYS X 11 34.298 48.740 49.230 1.00 37.13 C ATOM 24 NZ LYS X 11 34.377 50.245 49.264 1.00 38.00 N ATOM 25 C LYS X 11 30.334 44.201 51.310 1.00 33.41 C ATOM 26 O LYS X 11 29.103 44.242 51.386 1.00 33.19 O ATOM 27 N GLU X 12 31.112 43.560 52.185 1.00 32.40 N ATOM 28 CA GLU X 12 30.603 42.748 53.296 1.00 31.60 C ATOM 29 CB GLU X 12 31.765 42.159 54.113 1.00 31.72 C ATOM 30 CG GLU X 12 32.593 41.085 53.398 1.00 32.86 C ATOM 31 CD GLU X 12 33.959 40.834 54.062 1.00 33.84 C ATOM 32 OE1 GLU X 12 34.513 41.763 54.708 1.00 35.58 O ATOM 33 OE2 GLU X 12 34.482 39.698 53.936 1.00 36.48 O ATOM 34 C GLU X 12 29.695 43.552 54.215 1.00 30.09 C ATOM 35 O GLU X 12 28.652 43.063 54.652 1.00 29.50 O ATOM 36 N GLU X 13 30.110 44.786 54.497 1.00 28.75 N ATOM 37 CA GLU X 13 29.368 45.693 55.363 1.00 27.76 C ATOM 38 CB GLU X 13 30.201 46.943 55.688 1.00 28.33 C ATOM 39 CG GLU X 13 31.614 46.666 56.196 1.00 31.49 C ATOM 40 CD GLU X 13 32.670 46.790 55.104 1.00 34.76 C ATOM 41 OE1 GLU X 13 32.827 45.844 54.295 1.00 35.71 O ATOM 42 OE2 GLU X 13 33.353 47.840 55.066 1.00 36.51 O ATOM 43 C GLU X 13 28.034 46.111 54.742 1.00 25.85 C ATOM 44 O GLU X 13 27.083 46.394 55.461 1.00 25.59 O ATOM 45 N ASP X 14 27.976 46.155 53.411 1.00 24.16 N ATOM 46 CA ASP X 14 26.740 46.506 52.703 1.00 22.20 C ATOM 47 CB ASP X 14 27.023 46.949 51.268 1.00 22.02 C ATOM 48 CG ASP X 14 27.631 48.353 51.193 1.00 21.77 C ATOM 49 OD1 ASP X 14 27.367 49.190 52.084 1.00 21.02 O ATOM 50 OD2 ASP X 14 28.369 48.623 50.230 1.00 20.85 O ATOM 51 C ASP X 14 25.770 45.334 52.733 1.00 21.02 C ATOM 52 O ASP X 14 24.564 45.525 52.896 1.00 20.55 O ATOM 53 N VAL X 15 26.308 44.125 52.600 1.00 19.77 N ATOM 54 CA VAL X 15 25.510 42.910 52.751 1.00 19.38 C ATOM 55 CB VAL X 15 26.347 41.624 52.543 1.00 19.36 C ATOM 56 CG1 VAL X 15 25.496 40.377 52.830 1.00 19.68 C ATOM 57 CG2 VAL X 15 26.907 41.570 51.119 1.00 19.73 C ATOM 58 C VAL X 15 24.831 42.888 54.124 1.00 18.45 C ATOM 59 O VAL X 15 23.650 42.595 54.224 1.00 18.63 O ATOM 60 N LEU X 16 25.577 43.230 55.172 1.00 17.93 N ATOM 61 CA LEU X 16 25.030 43.237 56.534 1.00 17.17 C ATOM 62 CB LEU X 16 26.152 43.372 57.569 1.00 17.29 C ATOM 63 CG LEU X 16 27.107 42.181 57.710 1.00 17.24 C ATOM 64 CD1 LEU X 16 28.212 42.527 58.702 1.00 18.83 C ATOM 65 CD2 LEU X 16 26.363 40.935 58.147 1.00 17.49 C ATOM 66 C LEU X 16 23.966 44.311 56.746 1.00 16.62 C ATOM 67 O LEU X 16 23.010 44.097 57.488 1.00 16.22 O ATOM 68 N LYS X 17 24.148 45.465 56.102 1.00 16.26 N ATOM 69 CA LYS X 17 23.145 46.536 56.085 1.00 15.87 C ATOM 70 CB LYS X 17 23.700 47.779 55.381 1.00 15.92 C ATOM 71 CG LYS X 17 24.573 48.661 56.265 1.00 16.73 C ATOM 72 CD LYS X 17 24.935 49.962 55.557 1.00 16.22 C ATOM 73 CE LYS X 17 25.643 50.911 56.513 1.00 19.22 C ATOM 74 NZ LYS X 17 26.950 50.349 56.984 1.00 19.58 N ATOM 75 C LYS X 17 21.862 46.086 55.390 1.00 15.47 C ATOM 76 O LYS X 17 20.762 46.266 55.921 1.00 15.51 O ATOM 77 N PHE X 18 22.016 45.508 54.199 1.00 15.19 N ATOM 78 CA PHE X 18 20.905 44.929 53.450 1.00 15.34 C ATOM 79 CB PHE X 18 21.428 44.207 52.203 1.00 15.08 C ATOM 80 CG PHE X 18 21.768 45.112 51.036 1.00 15.12 C ATOM 81 CD1 PHE X 18 20.991 46.227 50.727 1.00 14.63 C ATOM 82 CE1 PHE X 18 21.296 47.039 49.623 1.00 14.97 C ATOM 83 CZ PHE X 18 22.386 46.729 48.817 1.00 15.27 C ATOM 84 CE2 PHE X 18 23.165 45.610 49.108 1.00 16.09 C ATOM 85 CD2 PHE X 18 22.847 44.805 50.214 1.00 15.18 C ATOM 86 C PHE X 18 20.130 43.926 54.316 1.00 15.81 C ATOM 87 O PHE X 18 18.894 43.961 54.385 1.00 15.46 O ATOM 88 N LEU X 19 20.868 43.027 54.965 1.00 16.26 N ATOM 89 CA LEU X 19 20.259 42.005 55.827 1.00 16.69 C ATOM 90 CB LEU X 19 21.319 40.977 56.266 1.00 16.82 C ATOM 91 CG LEU X 19 21.353 40.022 57.478 1.00 17.48 C ATOM 92 CD1 LEU X 19 20.113 39.951 58.393 1.00 17.46 C ATOM 93 CD2 LEU X 19 21.775 38.645 56.993 1.00 16.57 C ATOM 94 C LEU X 19 19.468 42.588 56.994 1.00 16.54 C ATOM 95 O LEU X 19 18.319 42.200 57.209 1.00 16.87 O ATOM 96 N ALA X 20 20.068 43.533 57.717 1.00 16.46 N ATOM 97 CA ALA X 20 19.408 44.199 58.849 1.00 16.22 C ATOM 98 CB ALA X 20 20.374 45.177 59.541 1.00 16.21 C ATOM 99 C ALA X 20 18.118 44.914 58.440 1.00 16.31 C ATOM 100 O ALA X 20 17.133 44.907 59.191 1.00 16.26 O ATOM 101 N ALA X 21 18.125 45.513 57.246 1.00 15.98 N ATOM 102 CA ALA X 21 16.961 46.240 56.726 1.00 15.51 C ATOM 103 CB ALA X 21 17.404 47.320 55.750 1.00 15.57 C ATOM 104 C ALA X 21 15.923 45.336 56.070 1.00 15.27 C ATOM 105 O ALA X 21 14.822 45.787 55.762 1.00 15.73 O ATOM 106 N GLY X 22 16.273 44.068 55.855 1.00 14.77 N ATOM 107 CA GLY X 22 15.408 43.130 55.144 1.00 14.09 C ATOM 108 C GLY X 22 15.254 43.441 53.662 1.00 13.92 C ATOM 109 O GLY X 22 14.189 43.241 53.093 1.00 13.77 O ATOM 110 N THR X 23 16.323 43.915 53.029 1.00 13.85 N ATOM 111 CA THR X 23 16.268 44.338 51.618 1.00 13.68 C ATOM 112 CB THR X 23 17.522 45.161 51.240 1.00 13.54 C ATOM 113 OG1 THR X 23 17.609 46.295 52.118 1.00 12.71 O ATOM 114 CG2 THR X 23 17.464 45.654 49.782 1.00 13.41 C ATOM 115 C THR X 23 15.994 43.164 50.649 1.00 13.97 C ATOM 116 O THR X 23 15.357 43.337 49.593 1.00 13.34 O ATOM 117 N HIS X 24 16.425 41.971 51.058 1.00 13.71 N ATOM 118 CA HIS X 24 16.247 40.734 50.289 1.00 14.30 C ATOM 119 CB HIS X 24 17.132 39.631 50.892 1.00 14.16 C ATOM 120 CG HIS X 24 16.844 39.364 52.335 1.00 14.97 C ATOM 121 ND1 HIS X 24 16.114 38.273 52.761 1.00 16.93 N ATOM 122 CE1 HIS X 24 16.007 38.304 54.077 1.00 15.26 C ATOM 123 NE2 HIS X 24 16.627 39.382 54.520 1.00 15.35 N ATOM 124 CD2 HIS X 24 17.157 40.064 53.451 1.00 14.02 C ATOM 125 C HIS X 24 14.806 40.226 50.268 1.00 14.30 C ATOM 126 O HIS X 24 14.491 39.283 49.543 1.00 13.87 O ATOM 127 N LEU X 25 13.943 40.834 51.078 1.00 14.73 N ATOM 128 CA LEU X 25 12.592 40.330 51.264 1.00 15.36 C ATOM 129 CB LEU X 25 12.061 40.685 52.661 1.00 15.55 C ATOM 130 CG LEU X 25 12.652 39.923 53.848 1.00 16.91 C ATOM 131 CD1 LEU X 25 12.091 40.470 55.160 1.00 19.07 C ATOM 132 CD2 LEU X 25 12.398 38.410 53.737 1.00 18.51 C ATOM 133 C LEU X 25 11.638 40.823 50.185 1.00 15.63 C ATOM 134 O LEU X 25 11.312 42.004 50.121 1.00 15.72 O ATOM 135 N GLY X 26 11.209 39.907 49.326 1.00 16.10 N ATOM 136 CA GLY X 26 10.210 40.217 48.319 1.00 16.93 C ATOM 137 C GLY X 26 8.854 39.855 48.874 1.00 17.73 C ATOM 138 O GLY X 26 8.695 39.668 50.093 1.00 17.81 O ATOM 139 N GLY X 27 7.877 39.722 47.983 1.00 18.17 N ATOM 140 CA GLY X 27 6.539 39.289 48.374 1.00 18.99 C ATOM 141 C GLY X 27 6.245 37.900 47.842 1.00 19.48 C ATOM 142 O GLY X 27 7.140 37.213 47.359 1.00 19.37 O ATOM 143 N THR X 28 4.987 37.489 47.933 1.00 19.94 N ATOM 144 CA THR X 28 4.594 36.158 47.481 1.00 20.79 C ATOM 145 CB THR X 28 3.406 35.610 48.309 1.00 20.95 C ATOM 146 OG1 THR X 28 2.292 36.498 48.182 1.00 21.19 O ATOM 147 CG2 THR X 28 3.791 35.506 49.778 1.00 21.28 C ATOM 148 C THR X 28 4.278 36.106 45.983 1.00 20.78 C ATOM 149 O THR X 28 4.208 35.024 45.400 1.00 21.53 O ATOM 150 N ASN X 29 4.096 37.268 45.360 1.00 20.74 N ATOM 151 CA ASN X 29 3.851 37.336 43.915 1.00 20.58 C ATOM 152 CB ASN X 29 2.637 38.220 43.600 1.00 20.72 C ATOM 153 CG ASN X 29 1.342 37.667 44.182 1.00 21.81 C ATOM 154 OD1 ASN X 29 0.556 38.399 44.769 1.00 23.20 O ATOM 155 ND2 ASN X 29 1.129 36.368 44.035 1.00 22.09 N ATOM 156 C ASN X 29 5.067 37.807 43.123 1.00 20.50 C ATOM 157 O ASN X 29 5.863 38.625 43.605 1.00 20.32 O ATOM 158 N LEU X 30 5.195 37.287 41.904 1.00 20.02 N ATOM 159 CA LEU X 30 6.341 37.562 41.064 1.00 20.09 C ATOM 160 CB LEU X 30 7.237 36.322 40.962 1.00 19.88 C ATOM 161 CG LEU X 30 8.521 36.381 40.122 1.00 19.08 C ATOM 162 CD1 LEU X 30 9.545 37.339 40.725 1.00 16.09 C ATOM 163 CD2 LEU X 30 9.115 34.973 39.968 1.00 19.47 C ATOM 164 C LEU X 30 5.923 38.034 39.674 1.00 20.50 C ATOM 165 O LEU X 30 5.246 37.310 38.932 1.00 20.79 O ATOM 166 N ASP X 31 6.324 39.256 39.341 1.00 20.38 N ATOM 167 CA ASP X 31 6.194 39.779 37.991 1.00 20.64 C ATOM 168 CB ASP X 31 6.471 41.285 37.985 1.00 20.84 C ATOM 169 CG ASP X 31 6.192 41.916 36.639 1.00 21.00 C ATOM 170 OD1 ASP X 31 7.080 41.876 35.763 1.00 20.07 O ATOM 171 OD2 ASP X 31 5.070 42.435 36.463 1.00 22.23 O ATOM 172 C ASP X 31 7.195 39.056 37.096 1.00 20.79 C ATOM 173 O ASP X 31 8.330 38.805 37.511 1.00 20.52 O ATOM 174 N PHE X 32 6.785 38.717 35.874 1.00 21.02 N ATOM 175 CA PHE X 32 7.645 37.926 34.984 1.00 21.44 C ATOM 176 CB PHE X 32 6.906 37.497 33.695 1.00 22.33 C ATOM 177 CG PHE X 32 6.743 38.594 32.666 1.00 24.17 C ATOM 178 CD1 PHE X 32 7.513 38.594 31.506 1.00 25.79 C ATOM 179 CE1 PHE X 32 7.358 39.601 30.538 1.00 25.74 C ATOM 180 CZ PHE X 32 6.424 40.610 30.729 1.00 24.87 C ATOM 181 CE2 PHE X 32 5.637 40.619 31.880 1.00 25.78 C ATOM 182 CD2 PHE X 32 5.796 39.610 32.838 1.00 26.21 C ATOM 183 C PHE X 32 8.992 38.603 34.693 1.00 20.92 C ATOM 184 O PHE X 32 10.004 37.932 34.499 1.00 20.47 O ATOM 185 N GLN X 33 9.003 39.933 34.695 1.00 20.63 N ATOM 186 CA GLN X 33 10.237 40.676 34.471 1.00 20.36 C ATOM 187 CB GLN X 33 9.944 42.054 33.877 1.00 20.69 C ATOM 188 CG GLN X 33 9.423 42.010 32.436 1.00 20.63 C ATOM 189 CD GLN X 33 9.380 43.389 31.796 1.00 20.80 C ATOM 190 OE1 GLN X 33 8.513 44.204 32.106 1.00 21.44 O ATOM 191 NE2 GLN X 33 10.330 43.658 30.909 1.00 20.99 N ATOM 192 C GLN X 33 11.117 40.781 35.723 1.00 19.86 C ATOM 193 O GLN X 33 12.277 41.182 35.631 1.00 20.34 O ATOM 194 N MET X 34 10.579 40.393 36.878 1.00 19.10 N ATOM 195 CA MET X 34 11.354 40.381 38.127 1.00 18.63 C ATOM 196 CB MET X 34 10.488 40.816 39.310 1.00 18.41 C ATOM 197 CG MET X 34 10.126 42.316 39.316 1.00 19.19 C ATOM 198 SD MET X 34 11.552 43.413 39.463 1.00 20.47 S ATOM 199 CE MET X 34 12.166 42.944 41.084 1.00 17.02 C ATOM 200 C MET X 34 12.022 39.030 38.421 1.00 18.36 C ATOM 201 O MET X 34 12.887 38.937 39.298 1.00 18.26 O ATOM 202 N GLU X 35 11.618 37.994 37.685 1.00 17.82 N ATOM 203 CA GLU X 35 12.170 36.642 37.836 1.00 17.39 C ATOM 204 CB GLU X 35 11.550 35.704 36.795 1.00 17.70 C ATOM 205 CG GLU X 35 12.027 34.254 36.892 1.00 17.81 C ATOM 206 CD GLU X 35 11.389 33.378 35.829 1.00 17.94 C ATOM 207 OE1 GLU X 35 10.451 32.639 36.179 1.00 21.18 O ATOM 208 OE2 GLU X 35 11.800 33.454 34.647 1.00 17.38 O ATOM 209 C GLU X 35 13.696 36.618 37.723 1.00 16.83 C ATOM 210 O GLU X 35 14.371 35.920 38.479 1.00 16.31 O ATOM 211 N GLN X 36 14.236 37.409 36.796 1.00 16.08 N ATOM 212 CA GLN X 36 15.683 37.514 36.610 1.00 15.59 C ATOM 213 CB GLN X 36 15.991 38.389 35.391 1.00 16.04 C ATOM 214 CG GLN X 36 15.618 39.873 35.563 1.00 15.36 C ATOM 215 CD GLN X 36 15.634 40.641 34.251 1.00 16.46 C ATOM 216 OE1 GLN X 36 14.598 41.159 33.810 1.00 19.47 O ATOM 217 NE2 GLN X 36 16.796 40.710 33.618 1.00 14.17 N ATOM 218 C GLN X 36 16.440 38.053 37.836 1.00 15.31 C ATOM 219 O GLN X 36 17.664 37.941 37.897 1.00 15.21 O ATOM 220 N TYR X 37 15.718 38.622 38.802 1.00 15.03 N ATOM 221 CA TYR X 37 16.348 39.258 39.966 1.00 15.39 C ATOM 222 CB TYR X 37 15.865 40.705 40.128 1.00 15.46 C ATOM 223 CG TYR X 37 16.080 41.585 38.917 1.00 15.82 C ATOM 224 CD1 TYR X 37 17.351 41.750 38.370 1.00 16.70 C ATOM 225 CE1 TYR X 37 17.555 42.558 37.262 1.00 16.54 C ATOM 226 CZ TYR X 37 16.485 43.223 36.694 1.00 16.10 C ATOM 227 OH TYR X 37 16.707 44.023 35.596 1.00 17.59 O ATOM 228 CE2 TYR X 37 15.213 43.085 37.213 1.00 15.68 C ATOM 229 CD2 TYR X 37 15.017 42.265 38.328 1.00 15.46 C ATOM 230 C TYR X 37 16.137 38.515 41.290 1.00 15.38 C ATOM 231 O TYR X 37 16.695 38.904 42.309 1.00 15.60 O ATOM 232 N ILE X 38 15.317 37.471 41.287 1.00 15.50 N ATOM 233 CA ILE X 38 15.089 36.707 42.514 1.00 15.21 C ATOM 234 CB ILE X 38 13.597 36.289 42.698 1.00 15.28 C ATOM 235 CG1 ILE X 38 13.089 35.425 41.538 1.00 14.69 C ATOM 236 CD1 ILE X 38 13.431 33.960 41.673 1.00 14.29 C ATOM 237 CG2 ILE X 38 12.714 37.517 42.868 1.00 14.60 C ATOM 238 C ILE X 38 16.072 35.529 42.607 1.00 15.56 C ATOM 239 O ILE X 38 16.654 35.113 41.605 1.00 15.11 O ATOM 240 N TYR X 39 16.279 35.028 43.818 1.00 15.67 N ATOM 241 CA TYR X 39 17.228 33.950 44.042 1.00 15.96 C ATOM 242 CB TYR X 39 18.187 34.303 45.189 1.00 16.07 C ATOM 243 CG TYR X 39 19.314 33.315 45.370 1.00 16.45 C ATOM 244 CD1 TYR X 39 20.390 33.287 44.493 1.00 15.53 C ATOM 245 CE1 TYR X 39 21.435 32.370 44.652 1.00 16.33 C ATOM 246 CZ TYR X 39 21.406 31.467 45.709 1.00 16.89 C ATOM 247 OH TYR X 39 22.434 30.559 45.882 1.00 17.23 O ATOM 248 CE2 TYR X 39 20.346 31.474 46.598 1.00 18.39 C ATOM 249 CD2 TYR X 39 19.299 32.400 46.423 1.00 17.79 C ATOM 250 C TYR X 39 16.486 32.656 44.326 1.00 16.39 C ATOM 251 O TYR X 39 16.754 31.635 43.698 1.00 16.56 O ATOM 252 N LYS X 40 15.538 32.709 45.255 1.00 16.77 N ATOM 253 CA LYS X 40 14.749 31.542 45.619 1.00 18.18 C ATOM 254 CB LYS X 40 15.553 30.572 46.508 1.00 17.81 C ATOM 255 CG LYS X 40 16.002 31.115 47.861 1.00 19.20 C ATOM 256 CD LYS X 40 16.748 30.032 48.639 1.00 20.03 C ATOM 257 CE LYS X 40 17.819 30.608 49.544 1.00 23.88 C ATOM 258 NZ LYS X 40 17.274 31.054 50.853 1.00 27.30 N ATOM 259 C LYS X 40 13.452 31.939 46.301 1.00 18.48 C ATOM 260 O LYS X 40 13.217 33.116 46.591 1.00 18.10 O ATOM 261 N ARG X 41 12.614 30.941 46.545 1.00 19.31 N ATOM 262 CA ARG X 41 11.348 31.141 47.209 1.00 20.40 C ATOM 263 CB ARG X 41 10.195 30.819 46.255 1.00 20.38 C ATOM 264 CG ARG X 41 8.848 31.278 46.746 1.00 20.74 C ATOM 265 CD ARG X 41 7.710 30.805 45.855 1.00 21.62 C ATOM 266 NE ARG X 41 6.526 31.626 46.106 1.00 22.97 N ATOM 267 CZ ARG X 41 5.346 31.478 45.506 1.00 24.70 C ATOM 268 NH1 ARG X 41 5.158 30.515 44.604 1.00 24.36 N ATOM 269 NH2 ARG X 41 4.347 32.301 45.816 1.00 23.10 N ATOM 270 C ARG X 41 11.316 30.246 48.441 1.00 21.24 C ATOM 271 O ARG X 41 11.756 29.100 48.394 1.00 21.61 O ATOM 272 N LYS X 42 10.826 30.794 49.545 1.00 22.35 N ATOM 273 CA LYS X 42 10.681 30.067 50.802 1.00 23.26 C ATOM 274 CB LYS X 42 10.827 31.052 51.971 1.00 23.59 C ATOM 275 CG LYS X 42 12.228 31.651 52.133 1.00 25.84 C ATOM 276 CD LYS X 42 13.248 30.571 52.524 1.00 27.90 C ATOM 277 CE LYS X 42 14.568 31.194 52.942 1.00 30.69 C ATOM 278 NZ LYS X 42 15.575 30.186 53.410 1.00 30.76 N ATOM 279 C LYS X 42 9.318 29.365 50.872 1.00 23.30 C ATOM 280 O LYS X 42 8.410 29.685 50.095 1.00 23.09 O ATOM 281 N SER X 43 9.169 28.428 51.814 1.00 23.50 N ATOM 282 CA SER X 43 7.908 27.695 51.989 1.00 23.42 C ATOM 283 CB SER X 43 8.049 26.560 53.010 1.00 23.74 C ATOM 284 OG SER X 43 8.248 27.068 54.317 1.00 23.90 O ATOM 285 C SER X 43 6.737 28.609 52.357 1.00 23.27 C ATOM 286 O SER X 43 5.589 28.311 52.011 1.00 23.41 O ATOM 287 N ASP X 44 7.020 29.730 53.028 1.00 22.61 N ATOM 288 CA ASP X 44 5.976 30.734 53.285 1.00 22.23 C ATOM 289 CB ASP X 44 6.345 31.679 54.445 1.00 22.20 C ATOM 290 CG ASP X 44 7.537 32.597 54.136 1.00 22.55 C ATOM 291 OD1 ASP X 44 8.189 32.457 53.077 1.00 22.52 O ATOM 292 OD2 ASP X 44 7.825 33.472 54.982 1.00 22.35 O ATOM 293 C ASP X 44 5.568 31.518 52.033 1.00 22.07 C ATOM 294 O ASP X 44 4.736 32.426 52.108 1.00 22.37 O ATOM 295 N GLY X 45 6.153 31.164 50.890 1.00 21.50 N ATOM 296 CA GLY X 45 5.794 31.773 49.609 1.00 21.11 C ATOM 297 C GLY X 45 6.583 33.021 49.241 1.00 20.57 C ATOM 298 O GLY X 45 6.484 33.503 48.117 1.00 20.80 O ATOM 299 N ILE X 46 7.371 33.533 50.184 1.00 20.14 N ATOM 300 CA ILE X 46 8.111 34.777 50.001 1.00 19.66 C ATOM 301 CB ILE X 46 8.604 35.356 51.371 1.00 19.98 C ATOM 302 CG1 ILE X 46 7.420 35.761 52.259 1.00 20.97 C ATOM 303 CD1 ILE X 46 6.553 36.913 51.710 1.00 23.35 C ATOM 304 CG2 ILE X 46 9.539 36.547 51.184 1.00 20.10 C ATOM 305 C ILE X 46 9.286 34.561 49.048 1.00 19.00 C ATOM 306 O ILE X 46 10.089 33.643 49.238 1.00 18.99 O ATOM 307 N TYR X 47 9.364 35.392 48.010 1.00 18.11 N ATOM 308 CA TYR X 47 10.515 35.391 47.103 1.00 17.53 C ATOM 309 CB TYR X 47 10.138 36.007 45.748 1.00 17.56 C ATOM 310 CG TYR X 47 9.407 35.050 44.833 1.00 17.37 C ATOM 311 CD1 TYR X 47 10.105 34.085 44.108 1.00 16.49 C ATOM 312 CE1 TYR X 47 9.450 33.203 43.273 1.00 17.17 C ATOM 313 CZ TYR X 47 8.072 33.270 43.148 1.00 17.59 C ATOM 314 OH TYR X 47 7.432 32.389 42.310 1.00 18.79 O ATOM 315 CE2 TYR X 47 7.347 34.211 43.854 1.00 17.39 C ATOM 316 CD2 TYR X 47 8.023 35.101 44.698 1.00 17.42 C ATOM 317 C TYR X 47 11.696 36.139 47.715 1.00 16.85 C ATOM 318 O TYR X 47 11.536 37.249 48.223 1.00 17.33 O ATOM 319 N ILE X 48 12.874 35.523 47.673 1.00 16.11 N ATOM 320 CA ILE X 48 14.098 36.150 48.153 1.00 15.38 C ATOM 321 CB ILE X 48 15.071 35.132 48.821 1.00 15.62 C ATOM 322 CG1 ILE X 48 14.403 34.388 49.998 1.00 15.96 C ATOM 323 CD1 ILE X 48 13.777 35.287 51.092 1.00 16.52 C ATOM 324 CG2 ILE X 48 16.367 35.813 49.263 1.00 14.67 C ATOM 325 C ILE X 48 14.791 36.835 46.977 1.00 14.88 C ATOM 326 O ILE X 48 15.053 36.204 45.954 1.00 14.80 O ATOM 327 N ILE X 49 15.088 38.121 47.141 1.00 13.89 N ATOM 328 CA ILE X 49 15.728 38.929 46.097 1.00 13.24 C ATOM 329 CB ILE X 49 15.343 40.451 46.230 1.00 12.69 C ATOM 330 CG1 ILE X 49 13.830 40.614 46.035 1.00 13.47 C ATOM 331 CD1 ILE X 49 13.267 41.935 46.489 1.00 13.63 C ATOM 332 CG2 ILE X 49 16.090 41.313 45.203 1.00 12.29 C ATOM 333 C ILE X 49 17.242 38.727 46.109 1.00 12.73 C ATOM 334 O ILE X 49 17.871 38.772 47.163 1.00 12.68 O ATOM 335 N ASN X 50 17.818 38.504 44.930 1.00 12.16 N ATOM 336 CA ASN X 50 19.262 38.393 44.785 1.00 12.01 C ATOM 337 CB ASN X 50 19.611 37.753 43.432 1.00 11.98 C ATOM 338 CG ASN X 50 21.079 37.331 43.320 1.00 11.89 C ATOM 339 OD1 ASN X 50 21.404 36.384 42.600 1.00 15.09 O ATOM 340 ND2 ASN X 50 21.960 38.018 44.025 1.00 9.56 N ATOM 341 C ASN X 50 19.918 39.780 44.929 1.00 12.37 C ATOM 342 O ASN X 50 19.870 40.605 44.004 1.00 11.20 O ATOM 343 N LEU X 51 20.538 40.004 46.090 1.00 12.23 N ATOM 344 CA LEU X 51 21.182 41.277 46.440 1.00 13.15 C ATOM 345 CB LEU X 51 21.624 41.252 47.902 1.00 13.20 C ATOM 346 CG LEU X 51 20.766 41.890 49.001 1.00 15.03 C ATOM 347 CD1 LEU X 51 19.297 42.120 48.658 1.00 14.89 C ATOM 348 CD2 LEU X 51 20.920 41.148 50.315 1.00 15.20 C ATOM 349 C LEU X 51 22.360 41.684 45.555 1.00 13.09 C ATOM 350 O LEU X 51 22.717 42.860 45.495 1.00 13.04 O ATOM 351 N LYS X 52 22.975 40.721 44.882 1.00 13.36 N ATOM 352 CA LYS X 52 23.986 41.046 43.876 1.00 14.10 C ATOM 353 CB LYS X 52 24.653 39.779 43.329 1.00 14.53 C ATOM 354 CG LYS X 52 25.619 39.113 44.309 1.00 16.50 C ATOM 355 CD LYS X 52 25.517 37.577 44.252 1.00 20.60 C ATOM 356 CE LYS X 52 26.405 36.971 43.201 1.00 22.69 C ATOM 357 NZ LYS X 52 26.297 35.476 43.131 1.00 23.72 N ATOM 358 C LYS X 52 23.349 41.867 42.745 1.00 13.64 C ATOM 359 O LYS X 52 23.946 42.828 42.260 1.00 13.63 O ATOM 360 N ARG X 53 22.130 41.497 42.353 1.00 13.36 N ATOM 361 CA ARG X 53 21.369 42.255 41.355 1.00 13.56 C ATOM 362 CB ARG X 53 20.117 41.489 40.896 1.00 13.45 C ATOM 363 CG ARG X 53 20.372 40.050 40.410 1.00 14.27 C ATOM 364 CD ARG X 53 21.123 40.001 39.089 1.00 16.18 C ATOM 365 NE ARG X 53 22.574 40.044 39.260 1.00 18.49 N ATOM 366 CZ ARG X 53 23.343 38.984 39.506 1.00 20.09 C ATOM 367 NH1 ARG X 53 22.810 37.774 39.625 1.00 21.57 N ATOM 368 NH2 ARG X 53 24.657 39.135 39.639 1.00 21.70 N ATOM 369 C ARG X 53 20.980 43.631 41.891 1.00 13.43 C ATOM 370 O ARG X 53 21.130 44.629 41.187 1.00 14.11 O ATOM 371 N THR X 54 20.478 43.677 43.125 1.00 13.10 N ATOM 372 CA THR X 54 20.211 44.945 43.823 1.00 12.92 C ATOM 373 CB THR X 54 19.922 44.718 45.334 1.00 12.81 C ATOM 374 OG1 THR X 54 18.834 43.803 45.479 1.00 11.60 O ATOM 375 CG2 THR X 54 19.571 46.037 46.059 1.00 12.38 C ATOM 376 C THR X 54 21.408 45.885 43.660 1.00 13.18 C ATOM 377 O THR X 54 21.251 47.019 43.188 1.00 13.06 O ATOM 378 N TRP X 55 22.597 45.386 44.007 1.00 13.14 N ATOM 379 CA TRP X 55 23.834 46.177 43.975 1.00 13.60 C ATOM 380 CB TRP X 55 24.991 45.418 44.642 1.00 14.08 C ATOM 381 CG TRP X 55 26.302 46.143 44.585 1.00 14.58 C ATOM 382 CD1 TRP X 55 27.410 45.786 43.866 1.00 15.56 C ATOM 383 NE1 TRP X 55 28.417 46.699 44.063 1.00 15.28 N ATOM 384 CE2 TRP X 55 27.976 47.669 44.925 1.00 15.31 C ATOM 385 CD2 TRP X 55 26.643 47.351 45.276 1.00 15.17 C ATOM 386 CE3 TRP X 55 25.948 48.199 46.155 1.00 15.54 C ATOM 387 CZ3 TRP X 55 26.600 49.324 46.650 1.00 15.72 C ATOM 388 CH2 TRP X 55 27.932 49.617 46.275 1.00 15.84 C ATOM 389 CZ2 TRP X 55 28.631 48.803 45.416 1.00 15.28 C ATOM 390 C TRP X 55 24.232 46.637 42.572 1.00 13.85 C ATOM 391 O TRP X 55 24.554 47.807 42.382 1.00 13.66 O ATOM 392 N GLU X 56 24.210 45.717 41.606 1.00 14.15 N ATOM 393 CA GLU X 56 24.508 46.040 40.204 1.00 15.20 C ATOM 394 CB GLU X 56 24.413 44.790 39.326 1.00 15.32 C ATOM 395 CG GLU X 56 25.568 43.814 39.528 1.00 18.48 C ATOM 396 CD GLU X 56 25.240 42.386 39.086 1.00 22.64 C ATOM 397 OE1 GLU X 56 24.247 42.186 38.337 1.00 22.16 O ATOM 398 OE2 GLU X 56 25.995 41.466 39.485 1.00 23.38 O ATOM 399 C GLU X 56 23.595 47.133 39.641 1.00 15.01 C ATOM 400 O GLU X 56 24.061 48.052 38.966 1.00 15.02 O ATOM 401 N LYS X 57 22.300 47.024 39.929 1.00 15.07 N ATOM 402 CA LYS X 57 21.301 47.966 39.421 1.00 15.43 C ATOM 403 CB LYS X 57 19.895 47.381 39.561 1.00 15.35 C ATOM 404 CG LYS X 57 19.644 46.192 38.645 1.00 16.72 C ATOM 405 CD LYS X 57 20.010 46.521 37.202 1.00 17.47 C ATOM 406 CE LYS X 57 19.612 45.391 36.285 1.00 19.69 C ATOM 407 NZ LYS X 57 20.159 45.554 34.911 1.00 21.09 N ATOM 408 C LYS X 57 21.389 49.322 40.110 1.00 15.45 C ATOM 409 O LYS X 57 21.097 50.357 39.513 1.00 15.28 O ATOM 410 N LEU X 58 21.802 49.304 41.370 1.00 15.48 N ATOM 411 CA LEU X 58 22.019 50.522 42.121 1.00 16.23 C ATOM 412 CB LEU X 58 22.405 50.146 43.543 1.00 17.25 C ATOM 413 CG LEU X 58 21.910 50.978 44.706 1.00 19.86 C ATOM 414 CD1 LEU X 58 21.822 50.054 45.930 1.00 21.77 C ATOM 415 CD2 LEU X 58 22.918 52.098 44.920 1.00 23.61 C ATOM 416 C LEU X 58 23.135 51.343 41.465 1.00 15.84 C ATOM 417 O LEU X 58 23.023 52.564 41.329 1.00 15.44 O ATOM 418 N LEU X 59 24.197 50.650 41.050 1.00 15.30 N ATOM 419 CA LEU X 59 25.343 51.274 40.381 1.00 15.32 C ATOM 420 CB LEU X 59 26.525 50.298 40.299 1.00 15.39 C ATOM 421 CG LEU X 59 27.225 49.909 41.609 1.00 16.30 C ATOM 422 CD1 LEU X 59 28.361 48.954 41.304 1.00 17.87 C ATOM 423 CD2 LEU X 59 27.759 51.147 42.346 1.00 17.69 C ATOM 424 C LEU X 59 24.984 51.781 38.990 1.00 14.82 C ATOM 425 O LEU X 59 25.414 52.862 38.596 1.00 14.71 O ATOM 426 N LEU X 60 24.200 50.990 38.258 1.00 14.35 N ATOM 427 CA LEU X 60 23.679 51.394 36.956 1.00 14.01 C ATOM 428 CB LEU X 60 22.914 50.241 36.303 1.00 14.39 C ATOM 429 CG LEU X 60 22.455 50.460 34.858 1.00 16.16 C ATOM 430 CD1 LEU X 60 23.637 50.340 33.877 1.00 18.26 C ATOM 431 CD2 LEU X 60 21.356 49.482 34.489 1.00 18.54 C ATOM 432 C LEU X 60 22.786 52.642 37.079 1.00 13.61 C ATOM 433 O LEU X 60 22.914 53.578 36.287 1.00 13.71 O ATOM 434 N ALA X 61 21.897 52.655 38.073 1.00 12.97 N ATOM 435 CA ALA X 61 21.060 53.822 38.350 1.00 13.04 C ATOM 436 CB ALA X 61 20.036 53.513 39.434 1.00 12.61 C ATOM 437 C ALA X 61 21.877 55.066 38.719 1.00 13.02 C ATOM 438 O ALA X 61 21.594 56.151 38.222 1.00 13.00 O ATOM 439 N ALA X 62 22.885 54.898 39.578 1.00 13.18 N ATOM 440 CA ALA X 62 23.757 56.007 40.009 1.00 13.51 C ATOM 441 CB ALA X 62 24.732 55.538 41.105 1.00 12.76 C ATOM 442 C ALA X 62 24.520 56.616 38.831 1.00 13.73 C ATOM 443 O ALA X 62 24.645 57.834 38.728 1.00 14.07 O ATOM 444 N ARG X 63 25.010 55.752 37.946 1.00 14.22 N ATOM 445 CA ARG X 63 25.668 56.151 36.709 1.00 15.34 C ATOM 446 CB ARG X 63 26.095 54.903 35.946 1.00 15.87 C ATOM 447 CG ARG X 63 27.355 55.055 35.133 1.00 20.75 C ATOM 448 CD ARG X 63 27.822 53.685 34.630 1.00 27.61 C ATOM 449 NE ARG X 63 28.044 52.743 35.733 1.00 31.32 N ATOM 450 CZ ARG X 63 27.836 51.430 35.658 1.00 33.90 C ATOM 451 NH1 ARG X 63 27.385 50.879 34.533 1.00 35.78 N ATOM 452 NH2 ARG X 63 28.070 50.662 36.714 1.00 34.66 N ATOM 453 C ARG X 63 24.760 57.011 35.836 1.00 14.90 C ATOM 454 O ARG X 63 25.177 58.068 35.357 1.00 15.56 O ATOM 455 N ALA X 64 23.520 56.561 35.647 1.00 14.15 N ATOM 456 CA ALA X 64 22.511 57.289 34.861 1.00 13.71 C ATOM 457 CB ALA X 64 21.251 56.435 34.699 1.00 13.54 C ATOM 458 C ALA X 64 22.148 58.638 35.478 1.00 13.18 C ATOM 459 O ALA X 64 21.916 59.619 34.766 1.00 12.87 O ATOM 460 N ILE X 65 22.079 58.668 36.804 1.00 12.69 N ATOM 461 CA ILE X 65 21.784 59.892 37.550 1.00 12.18 C ATOM 462 CB ILE X 65 21.468 59.566 39.040 1.00 12.27 C ATOM 463 CG1 ILE X 65 20.078 58.909 39.159 1.00 11.23 C ATOM 464 CD1 ILE X 65 19.843 58.174 40.493 1.00 11.69 C ATOM 465 CG2 ILE X 65 21.583 60.823 39.921 1.00 10.47 C ATOM 466 C ILE X 65 22.939 60.897 37.416 1.00 12.50 C ATOM 467 O ILE X 65 22.713 62.084 37.162 1.00 12.85 O ATOM 468 N VAL X 66 24.166 60.401 37.544 1.00 12.58 N ATOM 469 CA VAL X 66 25.378 61.223 37.451 1.00 12.76 C ATOM 470 CB VAL X 66 26.639 60.425 37.937 1.00 12.82 C ATOM 471 CG1 VAL X 66 27.954 61.116 37.565 1.00 14.01 C ATOM 472 CG2 VAL X 66 26.581 60.210 39.452 1.00 12.58 C ATOM 473 C VAL X 66 25.555 61.847 36.047 1.00 13.21 C ATOM 474 O VAL X 66 26.067 62.965 35.925 1.00 13.31 O ATOM 475 N ALA X 67 25.104 61.139 35.007 1.00 13.01 N ATOM 476 CA ALA X 67 25.190 61.637 33.630 1.00 13.38 C ATOM 477 CB ALA X 67 24.905 60.515 32.623 1.00 12.82 C ATOM 478 C ALA X 67 24.307 62.863 33.336 1.00 13.48 C ATOM 479 O ALA X 67 24.474 63.505 32.300 1.00 14.15 O ATOM 480 N ILE X 68 23.368 63.168 34.225 1.00 13.54 N ATOM 481 CA ILE X 68 22.514 64.349 34.071 1.00 13.57 C ATOM 482 CB ILE X 68 21.162 64.209 34.824 1.00 13.49 C ATOM 483 CG1 ILE X 68 20.448 62.901 34.446 1.00 12.37 C ATOM 484 CD1 ILE X 68 20.133 62.737 32.955 1.00 11.55 C ATOM 485 CG2 ILE X 68 20.266 65.423 34.558 1.00 13.63 C ATOM 486 C ILE X 68 23.281 65.566 34.573 1.00 13.94 C ATOM 487 O ILE X 68 23.572 65.677 35.769 1.00 13.16 O ATOM 488 N GLU X 69 23.595 66.466 33.639 1.00 14.41 N ATOM 489 CA GLU X 69 24.566 67.544 33.850 1.00 15.27 C ATOM 490 CB GLU X 69 24.909 68.221 32.518 1.00 15.50 C ATOM 491 CG GLU X 69 25.639 67.334 31.504 1.00 16.60 C ATOM 492 CD GLU X 69 24.703 66.604 30.543 1.00 17.99 C ATOM 493 OE1 GLU X 69 23.567 66.236 30.933 1.00 19.61 O ATOM 494 OE2 GLU X 69 25.109 66.388 29.384 1.00 18.22 O ATOM 495 C GLU X 69 24.121 68.584 34.886 1.00 15.47 C ATOM 496 O GLU X 69 24.940 69.068 35.672 1.00 15.95 O ATOM 497 N ASN X 70 22.836 68.933 34.882 1.00 15.17 N ATOM 498 CA ASN X 70 22.275 69.751 35.949 1.00 15.17 C ATOM 499 CB ASN X 70 21.362 70.836 35.367 1.00 15.33 C ATOM 500 CG ASN X 70 20.632 71.646 36.432 1.00 15.56 C ATOM 501 OD1 ASN X 70 20.926 71.565 37.620 1.00 15.17 O ATOM 502 ND2 ASN X 70 19.676 72.446 35.993 1.00 17.62 N ATOM 503 C ASN X 70 21.544 68.860 36.967 1.00 14.90 C ATOM 504 O ASN X 70 20.503 68.285 36.650 1.00 14.79 O ATOM 505 N PRO X 71 22.089 68.753 38.198 1.00 14.79 N ATOM 506 CA PRO X 71 21.547 67.844 39.229 1.00 14.65 C ATOM 507 CB PRO X 71 22.345 68.215 40.485 1.00 14.84 C ATOM 508 CG PRO X 71 23.598 68.814 39.977 1.00 15.24 C ATOM 509 CD PRO X 71 23.256 69.508 38.692 1.00 14.41 C ATOM 510 C PRO X 71 20.048 68.032 39.488 1.00 14.30 C ATOM 511 O PRO X 71 19.350 67.070 39.814 1.00 13.78 O ATOM 512 N ALA X 72 19.561 69.261 39.327 1.00 14.03 N ATOM 513 CA ALA X 72 18.148 69.576 39.557 1.00 13.57 C ATOM 514 CB ALA X 72 17.938 71.093 39.614 1.00 13.64 C ATOM 515 C ALA X 72 17.210 68.940 38.529 1.00 13.40 C ATOM 516 O ALA X 72 16.008 68.825 38.781 1.00 13.23 O ATOM 517 N ASP X 73 17.762 68.539 37.379 1.00 13.19 N ATOM 518 CA ASP X 73 16.992 67.870 36.320 1.00 13.44 C ATOM 519 CB ASP X 73 17.671 68.055 34.960 1.00 13.55 C ATOM 520 CG ASP X 73 17.528 69.477 34.437 1.00 14.70 C ATOM 521 OD1 ASP X 73 16.559 70.147 34.841 1.00 16.45 O ATOM 522 OD2 ASP X 73 18.368 69.922 33.628 1.00 15.65 O ATOM 523 C ASP X 73 16.697 66.387 36.591 1.00 13.25 C ATOM 524 O ASP X 73 16.021 65.727 35.797 1.00 13.01 O ATOM 525 N VAL X 74 17.209 65.880 37.711 1.00 12.99 N ATOM 526 CA VAL X 74 16.795 64.586 38.237 1.00 12.95 C ATOM 527 CB VAL X 74 17.971 63.819 38.895 1.00 13.13 C ATOM 528 CG1 VAL X 74 17.539 62.414 39.346 1.00 12.75 C ATOM 529 CG2 VAL X 74 19.169 63.731 37.938 1.00 12.47 C ATOM 530 C VAL X 74 15.671 64.841 39.251 1.00 13.27 C ATOM 531 O VAL X 74 15.836 65.580 40.226 1.00 13.05 O ATOM 532 N SER X 75 14.517 64.245 38.997 1.00 13.48 N ATOM 533 CA SER X 75 13.410 64.310 39.943 1.00 14.21 C ATOM 534 CB SER X 75 12.126 64.728 39.231 1.00 13.94 C ATOM 535 OG SER X 75 10.986 64.155 39.843 1.00 17.96 O ATOM 536 C SER X 75 13.266 62.989 40.705 1.00 13.94 C ATOM 537 O SER X 75 13.394 61.896 40.128 1.00 13.38 O ATOM 538 N VAL X 76 13.060 63.104 42.015 1.00 14.01 N ATOM 539 CA VAL X 76 13.006 61.945 42.903 1.00 14.17 C ATOM 540 CB VAL X 76 14.258 61.840 43.833 1.00 14.24 C ATOM 541 CG1 VAL X 76 15.510 61.544 43.024 1.00 13.27 C ATOM 542 CG2 VAL X 76 14.470 63.113 44.659 1.00 14.71 C ATOM 543 C VAL X 76 11.697 61.946 43.688 1.00 14.94 C ATOM 544 O VAL X 76 11.314 62.960 44.290 1.00 15.09 O ATOM 545 N ILE X 77 11.014 60.806 43.674 1.00 15.08 N ATOM 546 CA ILE X 77 9.609 60.757 44.050 1.00 16.01 C ATOM 547 CB ILE X 77 8.717 60.627 42.769 1.00 16.14 C ATOM 548 CG1 ILE X 77 8.608 61.995 42.082 1.00 16.19 C ATOM 549 CD1 ILE X 77 7.766 62.027 40.836 1.00 16.94 C ATOM 550 CG2 ILE X 77 7.342 60.053 43.094 1.00 16.76 C ATOM 551 C ILE X 77 9.261 59.679 45.088 1.00 16.04 C ATOM 552 O ILE X 77 9.762 58.556 45.028 1.00 15.42 O ATOM 553 N SER X 78 8.418 60.062 46.051 1.00 16.33 N ATOM 554 CA SER X 78 7.701 59.114 46.899 1.00 16.87 C ATOM 555 CB SER X 78 8.521 58.707 48.128 1.00 16.62 C ATOM 556 OG SER X 78 7.830 57.701 48.858 1.00 16.57 O ATOM 557 C SER X 78 6.358 59.684 47.346 1.00 17.55 C ATOM 558 O SER X 78 6.314 60.640 48.124 1.00 17.61 O ATOM 559 N SER X 79 5.267 59.096 46.858 1.00 18.08 N ATOM 560 CA SER X 79 3.942 59.458 47.347 1.00 19.01 C ATOM 561 CB SER X 79 2.848 58.949 46.405 1.00 18.84 C ATOM 562 OG SER X 79 2.953 57.547 46.234 1.00 19.69 O ATOM 563 C SER X 79 3.743 58.903 48.759 1.00 19.52 C ATOM 564 O SER X 79 3.237 59.598 49.640 1.00 19.93 O ATOM 565 N ARG X 80 4.157 57.651 48.964 1.00 19.99 N ATOM 566 CA ARG X 80 4.089 57.001 50.271 1.00 20.49 C ATOM 567 CB ARG X 80 4.571 55.554 50.179 1.00 20.42 C ATOM 568 CG ARG X 80 3.621 54.609 49.468 1.00 21.68 C ATOM 569 CD ARG X 80 4.310 53.283 49.195 1.00 22.90 C ATOM 570 NE ARG X 80 4.730 52.633 50.438 1.00 23.52 N ATOM 571 CZ ARG X 80 5.811 51.863 50.565 1.00 23.95 C ATOM 572 NH1 ARG X 80 6.602 51.639 49.520 1.00 23.31 N ATOM 573 NH2 ARG X 80 6.101 51.320 51.743 1.00 22.97 N ATOM 574 C ARG X 80 4.940 57.717 51.299 1.00 20.48 C ATOM 575 O ARG X 80 6.076 58.110 51.005 1.00 20.45 O ATOM 576 N ASN X 81 4.396 57.853 52.507 1.00 20.64 N ATOM 577 CA ASN X 81 5.142 58.389 53.654 1.00 21.24 C ATOM 578 CB ASN X 81 4.277 58.390 54.934 1.00 21.91 C ATOM 579 CG ASN X 81 3.675 57.020 55.258 1.00 24.36 C ATOM 580 OD1 ASN X 81 3.899 56.027 54.548 1.00 26.64 O ATOM 581 ND2 ASN X 81 2.888 56.964 56.344 1.00 27.50 N ATOM 582 C ASN X 81 6.472 57.678 53.901 1.00 20.73 C ATOM 583 O ASN X 81 7.453 58.306 54.297 1.00 20.84 O ATOM 584 N THR X 82 6.485 56.371 53.637 1.00 20.42 N ATOM 585 CA THR X 82 7.645 55.499 53.820 1.00 20.15 C ATOM 586 CB THR X 82 7.341 54.095 53.257 1.00 20.31 C ATOM 587 OG1 THR X 82 6.038 53.671 53.690 1.00 20.62 O ATOM 588 CG2 THR X 82 8.378 53.085 53.707 1.00 20.00 C ATOM 589 C THR X 82 8.927 56.034 53.165 1.00 19.82 C ATOM 590 O THR X 82 10.017 55.909 53.730 1.00 20.22 O ATOM 591 N GLY X 83 8.799 56.636 51.984 1.00 19.24 N ATOM 592 CA GLY X 83 9.968 57.147 51.264 1.00 18.71 C ATOM 593 C GLY X 83 10.131 58.655 51.220 1.00 18.35 C ATOM 594 O GLY X 83 11.050 59.160 50.577 1.00 18.24 O ATOM 595 N GLN X 84 9.248 59.385 51.901 1.00 18.52 N ATOM 596 CA GLN X 84 9.240 60.849 51.791 1.00 18.57 C ATOM 597 CB GLN X 84 7.937 61.456 52.333 1.00 18.49 C ATOM 598 CG GLN X 84 6.709 61.203 51.438 1.00 18.33 C ATOM 599 CD GLN X 84 5.432 61.846 51.970 1.00 19.54 C ATOM 600 OE1 GLN X 84 5.485 62.836 52.698 1.00 22.21 O ATOM 601 NE2 GLN X 84 4.277 61.289 51.603 1.00 19.68 N ATOM 602 C GLN X 84 10.487 61.486 52.413 1.00 18.38 C ATOM 603 O GLN X 84 11.149 62.299 51.774 1.00 18.47 O ATOM 604 N ARG X 85 10.827 61.097 53.639 1.00 18.38 N ATOM 605 CA ARG X 85 12.042 61.608 54.287 1.00 18.16 C ATOM 606 CB ARG X 85 12.153 61.069 55.714 1.00 18.58 C ATOM 607 CG ARG X 85 13.389 61.544 56.468 1.00 21.55 C ATOM 608 CD ARG X 85 13.255 61.267 57.955 1.00 26.37 C ATOM 609 NE ARG X 85 14.539 61.396 58.636 1.00 30.42 N ATOM 610 CZ ARG X 85 15.000 62.517 59.181 1.00 33.44 C ATOM 611 NH1 ARG X 85 14.275 63.637 59.144 1.00 34.31 N ATOM 612 NH2 ARG X 85 16.194 62.514 59.771 1.00 34.23 N ATOM 613 C ARG X 85 13.289 61.250 53.474 1.00 17.61 C ATOM 614 O ARG X 85 14.173 62.093 53.271 1.00 17.47 O ATOM 615 N ALA X 86 13.336 60.001 53.006 1.00 16.99 N ATOM 616 CA ALA X 86 14.429 59.496 52.176 1.00 16.60 C ATOM 617 CB ALA X 86 14.199 58.028 51.844 1.00 16.58 C ATOM 618 C ALA X 86 14.646 60.312 50.896 1.00 16.51 C ATOM 619 O ALA X 86 15.781 60.671 50.588 1.00 16.15 O ATOM 620 N VAL X 87 13.577 60.603 50.147 1.00 16.60 N ATOM 621 CA VAL X 87 13.745 61.381 48.899 1.00 16.58 C ATOM 622 CB VAL X 87 12.530 61.292 47.907 1.00 16.59 C ATOM 623 CG1 VAL X 87 12.280 59.849 47.481 1.00 16.75 C ATOM 624 CG2 VAL X 87 11.262 61.918 48.475 1.00 17.46 C ATOM 625 C VAL X 87 14.157 62.833 49.178 1.00 16.27 C ATOM 626 O VAL X 87 14.949 63.404 48.442 1.00 15.37 O ATOM 627 N LEU X 88 13.633 63.401 50.264 1.00 16.52 N ATOM 628 CA LEU X 88 14.032 64.735 50.720 1.00 16.66 C ATOM 629 CB LEU X 88 13.129 65.222 51.870 1.00 16.75 C ATOM 630 CG LEU X 88 11.665 65.514 51.490 1.00 17.60 C ATOM 631 CD1 LEU X 88 10.788 65.714 52.728 1.00 18.52 C ATOM 632 CD2 LEU X 88 11.546 66.726 50.558 1.00 17.78 C ATOM 633 C LEU X 88 15.511 64.790 51.099 1.00 16.61 C ATOM 634 O LEU X 88 16.208 65.740 50.731 1.00 16.55 O ATOM 635 N LYS X 89 15.990 63.761 51.802 1.00 16.68 N ATOM 636 CA LYS X 89 17.409 63.653 52.162 1.00 17.00 C ATOM 637 CB LYS X 89 17.630 62.549 53.213 1.00 17.52 C ATOM 638 CG LYS X 89 17.007 62.819 54.594 1.00 19.55 C ATOM 639 CD LYS X 89 17.681 63.987 55.327 1.00 24.32 C ATOM 640 CE LYS X 89 18.978 63.552 56.009 1.00 27.24 C ATOM 641 NZ LYS X 89 19.928 64.695 56.235 1.00 29.88 N ATOM 642 C LYS X 89 18.293 63.398 50.929 1.00 16.68 C ATOM 643 O LYS X 89 19.349 64.012 50.782 1.00 16.41 O ATOM 644 N PHE X 90 17.853 62.501 50.044 1.00 16.21 N ATOM 645 CA PHE X 90 18.581 62.222 48.796 1.00 15.79 C ATOM 646 CB PHE X 90 17.848 61.167 47.950 1.00 14.97 C ATOM 647 CG PHE X 90 18.538 60.845 46.644 1.00 14.54 C ATOM 648 CD1 PHE X 90 18.347 61.648 45.518 1.00 13.59 C ATOM 649 CE1 PHE X 90 18.988 61.358 44.308 1.00 13.40 C ATOM 650 CZ PHE X 90 19.825 60.253 44.211 1.00 13.33 C ATOM 651 CE2 PHE X 90 20.027 59.442 45.326 1.00 13.79 C ATOM 652 CD2 PHE X 90 19.377 59.735 46.537 1.00 13.59 C ATOM 653 C PHE X 90 18.750 63.513 47.997 1.00 15.78 C ATOM 654 O PHE X 90 19.836 63.799 47.489 1.00 15.87 O ATOM 655 N ALA X 91 17.671 64.284 47.893 1.00 15.93 N ATOM 656 CA ALA X 91 17.677 65.534 47.133 1.00 16.60 C ATOM 657 CB ALA X 91 16.265 66.091 47.005 1.00 16.62 C ATOM 658 C ALA X 91 18.623 66.575 47.729 1.00 16.94 C ATOM 659 O ALA X 91 19.407 67.184 47.001 1.00 17.28 O ATOM 660 N ALA X 92 18.564 66.759 49.048 1.00 17.44 N ATOM 661 CA ALA X 92 19.494 67.657 49.754 1.00 17.93 C ATOM 662 CB ALA X 92 19.179 67.698 51.252 1.00 18.27 C ATOM 663 C ALA X 92 20.946 67.254 49.529 1.00 18.02 C ATOM 664 O ALA X 92 21.815 68.112 49.327 1.00 18.62 O ATOM 665 N ALA X 93 21.200 65.947 49.534 1.00 17.75 N ATOM 666 CA ALA X 93 22.553 65.420 49.377 1.00 17.34 C ATOM 667 CB ALA X 93 22.613 63.982 49.878 1.00 17.36 C ATOM 668 C ALA X 93 23.115 65.516 47.957 1.00 17.08 C ATOM 669 O ALA X 93 24.318 65.681 47.773 1.00 17.15 O ATOM 670 N THR X 94 22.249 65.406 46.954 1.00 17.27 N ATOM 671 CA THR X 94 22.704 65.254 45.565 1.00 16.60 C ATOM 672 CB THR X 94 22.144 63.967 44.917 1.00 16.86 C ATOM 673 OG1 THR X 94 20.719 64.076 44.788 1.00 14.77 O ATOM 674 CG2 THR X 94 22.513 62.729 45.739 1.00 16.99 C ATOM 675 C THR X 94 22.361 66.424 44.643 1.00 16.72 C ATOM 676 O THR X 94 22.928 66.536 43.556 1.00 16.53 O ATOM 677 N GLY X 95 21.428 67.272 45.070 1.00 16.73 N ATOM 678 CA GLY X 95 20.979 68.408 44.262 1.00 16.94 C ATOM 679 C GLY X 95 19.783 68.106 43.369 1.00 17.04 C ATOM 680 O GLY X 95 19.370 68.953 42.570 1.00 16.56 O ATOM 681 N ALA X 96 19.230 66.898 43.505 1.00 16.94 N ATOM 682 CA ALA X 96 18.031 66.484 42.766 1.00 16.84 C ATOM 683 CB ALA X 96 17.851 64.970 42.864 1.00 16.42 C ATOM 684 C ALA X 96 16.775 67.207 43.278 1.00 16.99 C ATOM 685 O ALA X 96 16.772 67.750 44.382 1.00 16.89 O ATOM 686 N THR X 97 15.712 67.203 42.476 1.00 16.97 N ATOM 687 CA THR X 97 14.460 67.872 42.839 1.00 17.29 C ATOM 688 CB THR X 97 13.852 68.646 41.629 1.00 17.32 C ATOM 689 OG1 THR X 97 14.832 69.545 41.097 1.00 16.92 O ATOM 690 CG2 THR X 97 12.619 69.448 42.043 1.00 17.35 C ATOM 691 C THR X 97 13.457 66.857 43.380 1.00 17.63 C ATOM 692 O THR X 97 13.065 65.942 42.657 1.00 17.32 O ATOM 693 N PRO X 98 13.041 67.010 44.657 1.00 17.99 N ATOM 694 CA PRO X 98 12.136 66.017 45.229 1.00 18.76 C ATOM 695 CB PRO X 98 12.488 66.052 46.719 1.00 18.80 C ATOM 696 CG PRO X 98 12.880 67.521 46.972 1.00 18.29 C ATOM 697 CD PRO X 98 13.380 68.074 45.631 1.00 18.26 C ATOM 698 C PRO X 98 10.658 66.350 45.042 1.00 19.44 C ATOM 699 O PRO X 98 10.273 67.522 45.071 1.00 19.77 O ATOM 700 N ILE X 99 9.843 65.317 44.846 1.00 19.98 N ATOM 701 CA ILE X 99 8.400 65.440 45.017 1.00 20.65 C ATOM 702 CB ILE X 99 7.605 65.276 43.696 1.00 20.55 C ATOM 703 CG1 ILE X 99 8.132 66.222 42.608 1.00 20.58 C ATOM 704 CD1 ILE X 99 7.378 66.115 41.267 1.00 21.08 C ATOM 705 CG2 ILE X 99 6.112 65.513 43.949 1.00 20.67 C ATOM 706 C ILE X 99 7.949 64.406 46.042 1.00 20.95 C ATOM 707 O ILE X 99 7.700 63.239 45.710 1.00 21.31 O ATOM 708 N ALA X 100 7.869 64.844 47.295 1.00 21.40 N ATOM 709 CA ALA X 100 7.403 64.010 48.397 1.00 21.67 C ATOM 710 CB ALA X 100 8.216 64.307 49.662 1.00 21.55 C ATOM 711 C ALA X 100 5.925 64.281 48.640 1.00 21.93 C ATOM 712 O ALA X 100 5.504 65.439 48.737 1.00 22.20 O ATOM 713 N GLY X 101 5.140 63.216 48.738 1.00 22.03 N ATOM 714 CA GLY X 101 3.711 63.346 48.965 1.00 22.51 C ATOM 715 C GLY X 101 2.942 63.342 47.658 1.00 22.75 C ATOM 716 O GLY X 101 3.298 62.620 46.724 1.00 22.69 O ATOM 717 N ARG X 102 1.894 64.160 47.588 1.00 22.86 N ATOM 718 CA ARG X 102 0.992 64.146 46.437 1.00 23.05 C ATOM 719 CB ARG X 102 −0.275 64.963 46.702 1.00 23.67 C ATOM 720 CG ARG X 102 −1.432 64.587 45.760 1.00 27.17 C ATOM 721 CD ARG X 102 −2.557 65.621 45.778 1.00 33.00 C ATOM 722 NE ARG X 102 −3.551 65.360 44.731 1.00 36.11 N ATOM 723 CZ ARG X 102 −3.790 66.168 43.700 1.00 37.86 C ATOM 724 NH1 ARG X 102 −3.115 67.310 43.565 1.00 38.62 N ATOM 725 NH2 ARG X 102 −4.714 65.835 42.804 1.00 38.84 N ATOM 726 C ARG X 102 1.663 64.616 45.156 1.00 22.14 C ATOM 727 O ARG X 102 2.343 65.634 45.128 1.00 22.60 O ATOM 728 N PHE X 103 1.474 63.843 44.100 1.00 21.51 N ATOM 729 CA PHE X 103 1.874 64.238 42.764 1.00 20.69 C ATOM 730 CB PHE X 103 2.571 63.064 42.064 1.00 20.07 C ATOM 731 CG PHE X 103 3.002 63.348 40.647 1.00 19.32 C ATOM 732 CD1 PHE X 103 2.163 63.055 39.575 1.00 17.50 C ATOM 733 CE1 PHE X 103 2.565 63.302 38.269 1.00 17.80 C ATOM 734 CZ PHE X 103 3.819 63.844 38.021 1.00 18.07 C ATOM 735 CE2 PHE X 103 4.662 64.143 39.085 1.00 17.29 C ATOM 736 CD2 PHE X 103 4.256 63.889 40.384 1.00 17.28 C ATOM 737 C PHE X 103 0.579 64.626 42.058 1.00 20.64 C ATOM 738 O PHE X 103 −0.308 63.794 41.874 1.00 20.75 O ATOM 739 N THR X 104 0.464 65.901 41.700 1.00 20.41 N ATOM 740 CA THR X 104 −0.708 66.402 40.982 1.00 20.68 C ATOM 741 CB THR X 104 −0.745 67.951 40.979 1.00 20.76 C ATOM 742 OG1 THR X 104 −0.695 68.425 42.328 1.00 21.58 O ATOM 743 CG2 THR X 104 −2.018 68.469 40.306 1.00 21.15 C ATOM 744 C THR X 104 −0.710 65.882 39.547 1.00 20.16 C ATOM 745 O THR X 104 0.244 66.108 38.813 1.00 20.15 O ATOM 746 N PRO X 105 −1.781 65.175 39.147 1.00 20.15 N ATOM 747 CA PRO X 105 −1.871 64.682 37.768 1.00 20.04 C ATOM 748 CB PRO X 105 −3.275 64.069 37.707 1.00 19.98 C ATOM 749 CG PRO X 105 −3.564 63.675 39.143 1.00 20.49 C ATOM 750 CD PRO X 105 −2.953 64.781 39.952 1.00 20.10 C ATOM 751 C PRO X 105 −1.719 65.825 36.758 1.00 20.02 C ATOM 752 O PRO X 105 −2.322 66.879 36.934 1.00 19.57 O ATOM 753 N GLY X 106 −0.896 65.616 35.732 1.00 20.06 N ATOM 754 CA GLY X 106 −0.598 66.658 34.752 1.00 20.30 C ATOM 755 C GLY X 106 0.703 67.406 35.013 1.00 20.84 C ATOM 756 O GLY X 106 1.119 68.225 34.198 1.00 20.54 O ATOM 757 N THR X 107 1.349 67.140 36.145 1.00 21.12 N ATOM 758 CA THR X 107 2.638 67.778 36.462 1.00 21.98 C ATOM 759 CB THR X 107 3.228 67.252 37.797 1.00 21.60 C ATOM 760 OG1 THR X 107 2.318 67.541 38.861 1.00 21.06 O ATOM 761 CG2 THR X 107 4.575 67.899 38.116 1.00 22.08 C ATOM 762 C THR X 107 3.637 67.606 35.313 1.00 22.66 C ATOM 763 O THR X 107 4.340 68.558 34.951 1.00 22.79 O ATOM 764 N PHE X 108 3.678 66.405 34.735 1.00 23.33 N ATOM 765 CA PHE X 108 4.544 66.128 33.597 1.00 24.37 C ATOM 766 CB PHE X 108 5.146 64.712 33.669 1.00 23.71 C ATOM 767 CG PHE X 108 6.058 64.479 34.856 1.00 22.65 C ATOM 768 CD1 PHE X 108 6.694 65.540 35.501 1.00 20.74 C ATOM 769 CE1 PHE X 108 7.542 65.317 36.583 1.00 21.37 C ATOM 770 CZ PHE X 108 7.771 64.016 37.029 1.00 22.32 C ATOM 771 CE2 PHE X 108 7.151 62.946 36.384 1.00 21.63 C ATOM 772 CD2 PHE X 108 6.305 63.184 35.302 1.00 21.95 C ATOM 773 C PHE X 108 3.849 66.360 32.247 1.00 25.79 C ATOM 774 O PHE X 108 4.422 67.003 31.374 1.00 26.35 O ATOM 775 N THR X 109 2.628 65.849 32.077 1.00 27.27 N ATOM 776 CA THR X 109 1.922 65.940 30.780 1.00 28.86 C ATOM 777 CB THR X 109 0.732 64.950 30.677 1.00 28.57 C ATOM 778 OG1 THR X 109 −0.196 65.190 31.740 1.00 28.28 O ATOM 779 CG2 THR X 109 1.209 63.498 30.739 1.00 28.18 C ATOM 780 C THR X 109 1.433 67.356 30.412 1.00 30.37 C ATOM 781 O THR X 109 1.350 67.698 29.232 1.00 30.35 O ATOM 782 N ASN X 110 1.098 68.162 31.417 1.00 32.08 N ATOM 783 CA ASN X 110 0.687 69.553 31.201 1.00 34.07 C ATOM 784 CB ASN X 110 −0.083 70.068 32.419 1.00 34.27 C ATOM 785 CG ASN X 110 −1.571 69.980 32.252 1.00 35.53 C ATOM 786 OD1 ASN X 110 −2.180 70.876 31.681 1.00 38.58 O ATOM 787 ND2 ASN X 110 −2.178 68.926 32.782 1.00 36.18 N ATOM 788 C ASN X 110 1.899 70.447 30.958 1.00 35.02 C ATOM 789 O ASN X 110 2.430 71.048 31.888 1.00 35.57 O ATOM 790 N GLN X 111 2.352 70.534 29.716 1.00 36.38 N ATOM 791 CA GLN X 111 3.563 71.296 29.451 1.00 37.55 C ATOM 792 CB GLN X 111 4.713 70.375 29.047 1.00 37.86 C ATOM 793 CG GLN X 111 6.095 71.025 29.121 1.00 39.62 C ATOM 794 CD GLN X 111 6.402 71.623 30.483 1.00 41.90 C ATOM 795 OE1 GLN X 111 6.579 70.896 31.470 1.00 42.33 O ATOM 796 NE2 GLN X 111 6.470 72.961 30.544 1.00 41.48 N ATOM 797 C GLN X 111 3.369 72.420 28.445 1.00 37.98 C ATOM 798 O GLN X 111 2.883 72.203 27.335 1.00 38.21 O ATOM 799 N ILE X 112 3.776 73.619 28.860 1.00 38.65 N ATOM 800 CA ILE X 112 3.606 74.851 28.084 1.00 39.07 C ATOM 801 CB ILE X 112 3.508 76.088 29.018 1.00 39.13 C ATOM 802 CG1 ILE X 112 4.823 76.288 29.793 1.00 39.83 C ATOM 803 CD1 ILE X 112 4.848 77.481 30.741 1.00 39.68 C ATOM 804 CG2 ILE X 112 2.305 75.943 29.964 1.00 39.97 C ATOM 805 C ILE X 112 4.726 75.066 27.062 1.00 38.69 C ATOM 806 O ILE X 112 4.609 75.922 26.169 1.00 39.27 O ATOM 807 N GLN X 113 5.802 74.289 27.195 1.00 37.82 N ATOM 808 CA GLN X 113 6.989 74.443 26.361 1.00 36.64 C ATOM 809 CB GLN X 113 8.093 75.184 27.123 1.00 37.01 C ATOM 810 CG GLN X 113 8.202 76.660 26.774 1.00 38.82 C ATOM 811 CD GLN X 113 8.131 77.561 27.987 1.00 41.43 C ATOM 812 OE1 GLN X 113 8.776 77.310 29.010 1.00 42.62 O ATOM 813 NE2 GLN X 113 7.333 78.626 27.881 1.00 42.06 N ATOM 814 C GLN X 113 7.508 73.109 25.849 1.00 35.43 C ATOM 815 O GLN X 113 7.676 72.157 26.607 1.00 35.25 O ATOM 816 N ALA X 114 7.753 73.059 24.546 1.00 33.91 N ATOM 817 CA ALA X 114 8.380 71.913 23.902 1.00 32.18 C ATOM 818 CB ALA X 114 8.214 72.037 22.402 1.00 32.43 C ATOM 819 C ALA X 114 9.867 71.827 24.251 1.00 30.68 C ATOM 820 O ALA X 114 10.418 70.737 24.419 1.00 30.39 O ATOM 821 N ALA X 115 10.500 72.992 24.359 1.00 29.00 N ATOM 822 CA ALA X 115 11.949 73.098 24.490 1.00 27.55 C ATOM 823 CB ALA X 115 12.434 74.411 23.870 1.00 27.22 C ATOM 824 C ALA X 115 12.417 72.988 25.940 1.00 26.49 C ATOM 825 O ALA X 115 11.735 73.462 26.849 1.00 26.04 O ATOM 826 N PHE X 116 13.583 72.362 26.130 1.00 25.25 N ATOM 827 CA PHE X 116 14.278 72.280 27.432 1.00 24.37 C ATOM 828 CB PHE X 116 14.855 73.642 27.838 1.00 24.42 C ATOM 829 CG PHE X 116 15.704 74.256 26.770 1.00 24.56 C ATOM 830 CD1 PHE X 116 17.005 73.825 26.573 1.00 24.63 C ATOM 831 CE1 PHE X 116 17.786 74.373 25.562 1.00 25.42 C ATOM 832 CZ PHE X 116 17.258 75.350 24.734 1.00 24.56 C ATOM 833 CE2 PHE X 116 15.953 75.774 24.911 1.00 24.23 C ATOM 834 CD2 PHE X 116 15.183 75.228 25.925 1.00 24.58 C ATOM 835 C PHE X 116 13.406 71.671 28.514 1.00 23.76 C ATOM 836 O PHE X 116 13.328 72.152 29.650 1.00 23.31 O ATOM 837 N ARG X 117 12.764 70.583 28.116 1.00 22.95 N ATOM 838 CA ARG X 117 11.836 69.836 28.932 1.00 22.58 C ATOM 839 CB ARG X 117 11.229 68.739 28.063 1.00 23.11 C ATOM 840 CG ARG X 117 10.381 67.786 28.801 1.00 26.40 C ATOM 841 CD ARG X 117 9.223 67.388 27.956 1.00 31.69 C ATOM 842 NE ARG X 117 8.079 67.243 28.829 1.00 35.88 N ATOM 843 CZ ARG X 117 6.862 67.673 28.540 1.00 38.16 C ATOM 844 NH1 ARG X 117 6.624 68.282 27.380 1.00 38.49 N ATOM 845 NH2 ARG X 117 5.891 67.494 29.422 1.00 39.18 N ATOM 846 C ARG X 117 12.480 69.246 30.193 1.00 21.31 C ATOM 847 O ARG X 117 13.551 68.636 30.140 1.00 21.06 O ATOM 848 N GLU X 118 11.804 69.437 31.321 1.00 20.15 N ATOM 849 CA GLU X 118 12.299 69.005 32.618 1.00 19.48 C ATOM 850 CB GLU X 118 12.835 70.213 33.407 1.00 19.54 C ATOM 851 CG GLU X 118 14.101 70.874 32.785 1.00 20.03 C ATOM 852 CD GLU X 118 14.407 72.255 33.371 1.00 20.32 C ATOM 853 OE1 GLU X 118 13.702 73.220 33.011 1.00 20.60 O ATOM 854 OE2 GLU X 118 15.354 72.382 34.190 1.00 21.21 O ATOM 855 C GLU X 118 11.163 68.317 33.379 1.00 18.61 C ATOM 856 O GLU X 118 9.990 68.672 33.182 1.00 18.67 O ATOM 857 N PRO X 119 11.489 67.318 34.236 1.00 17.43 N ATOM 858 CA PRO X 119 12.808 66.702 34.476 1.00 16.34 C ATOM 859 CB PRO X 119 12.548 65.736 35.641 1.00 16.16 C ATOM 860 CG PRO X 119 11.102 65.507 35.639 1.00 16.78 C ATOM 861 CD PRO X 119 10.454 66.742 35.108 1.00 16.78 C ATOM 862 C PRO X 119 13.356 65.922 33.280 1.00 15.31 C ATOM 863 O PRO X 119 12.632 65.646 32.325 1.00 14.60 O ATOM 864 N ARG X 120 14.641 65.592 33.349 1.00 14.55 N ATOM 865 CA ARG X 120 15.301 64.782 32.329 1.00 14.10 C ATOM 866 CB ARG X 120 16.705 65.330 32.048 1.00 14.08 C ATOM 867 CG ARG X 120 16.724 66.721 31.427 1.00 13.92 C ATOM 868 CD ARG X 120 18.152 67.134 31.105 1.00 14.02 C ATOM 869 NE ARG X 120 18.705 66.304 30.043 1.00 13.76 N ATOM 870 CZ ARG X 120 19.992 66.008 29.906 1.00 15.31 C ATOM 871 NH1 ARG X 120 20.883 66.464 30.777 1.00 14.55 N ATOM 872 NH2 ARG X 120 20.387 65.236 28.895 1.00 17.13 N ATOM 873 C ARG X 120 15.391 63.327 32.768 1.00 13.55 C ATOM 874 O ARG X 120 15.600 62.440 31.953 1.00 13.14 O ATOM 875 N LEU X 121 15.227 63.089 34.064 1.00 13.36 N ATOM 876 CA LEU X 121 15.255 61.737 34.613 1.00 13.20 C ATOM 877 CB LEU X 121 16.690 61.319 34.958 1.00 13.15 C ATOM 878 CG LEU X 121 16.957 59.917 35.532 1.00 13.48 C ATOM 879 CD1 LEU X 121 16.618 58.803 34.528 1.00 12.75 C ATOM 880 CD2 LEU X 121 18.404 59.808 35.967 1.00 13.17 C ATOM 881 C LEU X 121 14.355 61.665 35.845 1.00 13.02 C ATOM 882 O LEU X 121 14.327 62.584 36.672 1.00 13.22 O ATOM 883 N LEU X 122 13.617 60.571 35.953 1.00 12.73 N ATOM 884 CA LEU X 122 12.721 60.361 37.073 1.00 12.88 C ATOM 885 CB LEU X 122 11.274 60.250 36.577 1.00 13.01 C ATOM 886 CG LEU X 122 10.217 59.856 37.614 1.00 13.55 C ATOM 887 CD1 LEU X 122 10.104 60.923 38.694 1.00 12.85 C ATOM 888 CD2 LEU X 122 8.867 59.587 36.953 1.00 12.51 C ATOM 889 C LEU X 122 13.117 59.117 37.871 1.00 13.13 C ATOM 890 O LEU X 122 13.364 58.048 37.306 1.00 12.99 O ATOM 891 N VAL X 123 13.206 59.266 39.187 1.00 13.02 N ATOM 892 CA VAL X 123 13.380 58.101 40.043 1.00 13.06 C ATOM 893 CB VAL X 123 14.895 57.858 40.471 1.00 13.01 C ATOM 894 CG1 VAL X 123 15.740 59.116 40.419 1.00 13.47 C ATOM 895 CG2 VAL X 123 15.045 57.072 41.765 1.00 13.75 C ATOM 896 C VAL X 123 12.300 58.002 41.135 1.00 13.24 C ATOM 897 O VAL X 123 12.051 58.960 41.875 1.00 13.28 O ATOM 898 N VAL X 124 11.620 56.858 41.176 1.00 13.15 N ATOM 899 CA VAL X 124 10.482 56.661 42.078 1.00 13.12 C ATOM 900 CB VAL X 124 9.117 56.447 41.328 1.00 13.16 C ATOM 901 CG1 VAL X 124 8.872 57.539 40.282 1.00 12.38 C ATOM 902 CG2 VAL X 124 9.041 55.066 40.687 1.00 12.89 C ATOM 903 C VAL X 124 10.709 55.527 43.079 1.00 13.18 C ATOM 904 O VAL X 124 11.514 54.619 42.847 1.00 12.76 O ATOM 905 N THR X 125 9.976 55.598 44.186 1.00 13.18 N ATOM 906 CA THR X 125 10.116 54.654 45.286 1.00 13.79 C ATOM 907 CB THR X 125 9.684 55.325 46.617 1.00 13.80 C ATOM 908 OG1 THR X 125 10.532 56.455 46.864 1.00 14.25 O ATOM 909 CG2 THR X 125 9.769 54.358 47.796 1.00 13.19 C ATOM 910 C THR X 125 9.338 53.356 45.065 1.00 13.98 C ATOM 911 O THR X 125 9.751 52.304 45.541 1.00 13.85 O ATOM 912 N ASP X 126 8.225 53.427 44.336 1.00 14.60 N ATOM 913 CA ASP X 126 7.270 52.319 44.286 1.00 15.58 C ATOM 914 CB ASP X 126 6.406 52.356 45.565 1.00 15.59 C ATOM 915 CG ASP X 126 5.375 51.218 45.657 1.00 16.75 C ATOM 916 OD1 ASP X 126 5.034 50.575 44.644 1.00 17.08 O ATOM 917 OD2 ASP X 126 4.887 50.981 46.782 1.00 18.47 O ATOM 918 C ASP X 126 6.418 52.463 43.032 1.00 16.06 C ATOM 919 O ASP X 126 5.566 53.342 42.976 1.00 16.09 O ATOM 920 N PRO X 127 6.645 51.606 42.016 1.00 16.67 N ATOM 921 CA PRO X 127 5.917 51.738 40.750 1.00 17.51 C ATOM 922 CB PRO X 127 6.471 50.580 39.903 1.00 17.42 C ATOM 923 CG PRO X 127 7.794 50.297 40.483 1.00 17.16 C ATOM 924 CD PRO X 127 7.594 50.483 41.961 1.00 16.89 C ATOM 925 C PRO X 127 4.401 51.612 40.883 1.00 18.08 C ATOM 926 O PRO X 127 3.671 52.136 40.042 1.00 18.48 O ATOM 927 N ARG X 128 3.951 50.928 41.935 1.00 18.42 N ATOM 928 CA ARG X 128 2.533 50.747 42.236 1.00 19.55 C ATOM 929 CB ARG X 128 2.374 49.541 43.187 1.00 20.14 C ATOM 930 CG ARG X 128 0.951 49.042 43.542 1.00 24.68 C ATOM 931 CD ARG X 128 0.144 48.414 42.369 1.00 31.10 C ATOM 932 NE ARG X 128 0.541 47.092 41.821 1.00 33.70 N ATOM 933 CZ ARG X 128 1.310 46.159 42.396 1.00 34.27 C ATOM 934 NH1 ARG X 128 1.838 46.322 43.601 1.00 34.54 N ATOM 935 NH2 ARG X 128 1.549 45.031 41.739 1.00 34.73 N ATOM 936 C ARG X 128 1.960 52.055 42.812 1.00 18.79 C ATOM 937 O ARG X 128 1.043 52.636 42.240 1.00 18.93 O ATOM 938 N ALA X 129 2.526 52.538 43.914 1.00 18.15 N ATOM 939 CA ALA X 129 2.052 53.771 44.539 1.00 17.79 C ATOM 940 CB ALA X 129 2.625 53.915 45.933 1.00 17.72 C ATOM 941 C ALA X 129 2.366 55.017 43.699 1.00 17.52 C ATOM 942 O ALA X 129 1.605 55.981 43.697 1.00 17.04 O ATOM 943 N ASP X 130 3.491 54.983 42.991 1.00 17.23 N ATOM 944 CA ASP X 130 3.935 56.117 42.188 1.00 17.23 C ATOM 945 CB ASP X 130 5.428 56.372 42.433 1.00 17.10 C ATOM 946 CG ASP X 130 5.725 56.714 43.882 1.00 17.70 C ATOM 947 OD1 ASP X 130 4.993 57.561 44.441 1.00 18.36 O ATOM 948 OD2 ASP X 130 6.677 56.145 44.466 1.00 16.55 O ATOM 949 C ASP X 130 3.635 55.911 40.707 1.00 17.03 C ATOM 950 O ASP X 130 4.374 56.375 39.846 1.00 16.69 O ATOM 951 N HIS X 131 2.535 55.219 40.416 1.00 17.32 N ATOM 952 CA HIS X 131 2.159 54.916 39.039 1.00 17.64 C ATOM 953 CB HIS X 131 0.936 53.987 38.998 1.00 18.00 C ATOM 954 CG HIS X 131 −0.354 54.683 39.299 1.00 19.71 C ATOM 955 ND1 HIS X 131 −0.868 54.777 49.576 1.00 21.28 N ATOM 956 CE1 HIS X 131 −1.997 55.464 40.545 1.00 22.13 C ATOM 957 NE2 HIS X 131 −2.233 55.820 39.294 1.00 23.52 N ATOM 958 CD2 HIS X 131 −1.220 55.344 38.494 1.00 20.68 C ATOM 959 C HIS X 131 1.897 56.177 38.201 1.00 17.25 C ATOM 960 O HIS X 131 2.167 56.183 36.997 1.00 17.72 O ATOM 961 N GLN X 132 1.368 57.231 38.830 1.00 16.69 N ATOM 962 CA GLN X 132 0.979 58.443 38.095 1.00 16.49 C ATOM 963 CB GLN X 132 0.175 59.428 38.969 1.00 16.53 C ATOM 964 CG GLN X 132 −0.312 60.706 38.239 1.00 16.83 C ATOM 965 CD GLN X 132 −1.490 60.452 37.299 1.00 17.26 C ATOM 966 OE1 GLN X 132 −2.359 59.630 37.585 1.00 19.31 O ATOM 967 NE2 GLN X 132 −1.524 61.162 36.179 1.00 15.76 N ATOM 968 C GLN X 132 2.181 59.111 37.406 1.00 16.10 C ATOM 969 O GLN X 132 2.157 59.255 36.188 1.00 16.02 O ATOM 970 N PRO X 133 3.229 59.514 38.174 1.00 15.88 N ATOM 971 CA PRO X 133 4.436 60.074 37.536 1.00 15.39 C ATOM 972 CB PRO X 133 5.405 60.294 38.711 1.00 15.57 C ATOM 973 CG PRO X 133 4.829 59.557 39.862 1.00 16.05 C ATOM 974 CD PRO X 133 3.350 59.520 39.645 1.00 15.63 C ATOM 975 C PRO X 133 5.085 59.161 36.503 1.00 14.99 C ATOM 976 O PRO X 133 5.538 59.652 35.478 1.00 15.33 O ATOM 977 N LEU X 134 5.130 57.856 36.772 1.00 14.60 N ATOM 978 CA LEU X 134 5.691 56.889 35.837 1.00 14.62 C ATOM 979 CB LEU X 134 5.780 55.485 36.462 1.00 14.13 C ATOM 980 CG LEU X 134 6.857 55.209 37.520 1.00 14.87 C ATOM 981 CD1 LEU X 134 6.728 53.784 38.025 1.00 13.28 C ATOM 982 CD2 LEU X 134 8.271 55.445 36.995 1.00 11.76 C ATOM 983 C LEU X 134 4.898 56.822 34.539 1.00 14.42 C ATOM 984 O LEU X 134 5.479 56.719 33.470 1.00 14.32 O ATOM 985 N THR X 135 3.572 56.861 34.634 1.00 14.68 N ATOM 986 CA THR X 135 2.752 56.780 33.431 1.00 14.87 C ATOM 987 CB THR X 135 1.281 56.455 33.743 1.00 14.78 C ATOM 988 OG1 THR X 135 1.227 55.306 34.594 1.00 16.39 O ATOM 989 CG2 THR X 135 0.511 56.155 32.461 1.00 14.96 C ATOM 990 C THR X 135 2.879 58.075 32.640 1.00 14.75 C ATOM 991 O THR X 135 3.002 58.040 31.423 1.00 14.14 O ATOM 992 N GLU X 136 2.876 59.207 33.347 1.00 14.94 N ATOM 993 CA GLU X 136 3.068 60.506 32.709 1.00 15.53 C ATOM 994 CB GLU X 136 2.861 61.666 33.701 1.00 15.40 C ATOM 995 CG GLU X 136 1.420 61.797 34.192 1.00 16.02 C ATOM 996 CD GLU X 136 1.098 63.150 34.818 1.00 16.58 C ATOM 997 OE1 GLU X 136 1.778 64.155 34.508 1.00 17.87 O ATOM 998 OE2 GLU X 136 0.149 63.203 35.627 1.00 17.77 O ATOM 999 C GLU X 136 4.442 60.577 32.049 1.00 15.56 C ATOM 1000 O GLU X 136 4.562 61.979 30.935 1.00 15.79 O ATOM 1001 N ALA X 137 5.463 60.045 32.727 1.00 15.57 N ATOM 1002 CA ALA X 137 6.819 59.965 32.175 1.00 16.00 C ATOM 1003 CB ALA X 137 7.767 59.301 33.173 1.00 15.62 C ATOM 1004 C ALA X 137 6.862 59.219 30.842 1.00 16.37 C ATOM 1005 O ALA X 137 7.570 59.625 29.916 1.00 16.31 O ATOM 1006 N SER X 138 6.115 58.120 30.763 1.00 16.92 N ATOM 1007 CA SER X 138 6.014 57.344 29.532 1.00 18.02 C ATOM 1008 CB SER X 138 5.191 56.079 29.748 1.00 18.15 C ATOM 1009 OG SER X 138 5.486 55.160 28.709 1.00 22.02 O ATOM 1010 C SER X 138 5.404 58.154 28.391 1.00 17.61 C ATOM 1011 O SER X 138 5.863 58.053 27.254 1.00 18.11 O ATOM 1012 N TYR X 139 4.377 58.949 28.700 1.00 17.08 N ATOM 1013 CA TYR X 139 3.731 59.827 27.713 1.00 16.78 C ATOM 1014 CB TYR X 139 2.561 60.592 28.352 1.00 16.51 C ATOM 1015 CG TYR X 139 1.251 59.842 28.403 1.00 16.85 C ATOM 1016 CD1 TYR X 139 1.211 58.465 28.644 1.00 17.24 C ATOM 1017 CE1 TYR X 139 0.000 57.783 28.700 1.00 16.10 C ATOM 1018 CZ TYR X 139 −1.175 58.475 28.530 1.00 15.99 C ATOM 1019 OH TYR X 139 −2.365 57.801 28.585 1.00 16.80 O ATOM 1020 CE2 TYR X 139 −1.166 59.836 28.302 1.00 16.70 C ATOM 1021 CD2 TYR X 139 0.043 60.513 28.245 1.00 16.75 C ATOM 1022 C TYR X 139 4.696 60.843 27.120 1.00 16.51 C ATOM 1023 O TYR X 139 4.581 61.191 25.952 1.00 16.27 O ATOM 1024 N VAL X 140 5.640 61.323 27.932 1.00 16.43 N ATOM 1025 CA VAL X 140 6.535 62.412 27.506 1.00 16.24 C ATOM 1026 CB VAL X 140 6.489 63.629 28.501 1.00 16.11 C ATOM 1027 CG1 VAL X 140 5.067 64.193 28.598 1.00 16.15 C ATOM 1028 CG2 VAL X 140 7.006 63.245 29.894 1.00 15.19 C ATOM 1029 C VAL X 140 7.983 61.970 27.222 1.00 16.22 C ATOM 1030 O VAL X 140 8.869 62.815 27.080 1.00 16.85 O ATOM 1031 N ASN X 141 8.206 60.657 27.129 1.00 16.23 N ATOM 1032 CA ASN X 141 9.540 60.066 26.927 1.00 16.12 C ATOM 1033 CB ASN X 141 10.080 60.353 25.510 1.00 16.81 C ATOM 1034 CG ASN X 141 9.140 59.874 24.405 1.00 18.10 C ATOM 1035 OD1 ASN X 141 8.430 58.882 24.558 1.00 21.41 O ATOM 1036 ND2 ASN X 141 9.146 60.579 23.284 1.00 19.83 N ATOM 1037 C ASN X 141 10.566 60.491 28.001 1.00 15.49 C ATOM 1038 O ASN X 141 11.721 60.799 27.700 1.00 15.25 O ATOM 1039 N LEU X 142 10.120 60.514 29.252 1.00 14.66 N ATOM 1040 CA LEU X 142 10.995 60.776 30.382 1.00 13.74 C ATOM 1041 CB LEU X 142 10.224 61.539 31.463 1.00 13.57 C ATOM 1042 CG LEU X 142 10.825 61.692 32.868 1.00 13.66 C ATOM 1043 CD1 LEU X 142 12.170 62.397 32.820 1.00 11.49 C ATOM 1044 CD2 LEU X 142 9.858 62.428 33.774 1.00 13.24 C ATOM 1045 C LEU X 142 11.510 59.422 30.900 1.00 13.56 C ATOM 1046 O LEU X 142 10.712 58.586 31.327 1.00 13.44 O ATOM 1047 N PRO X 143 12.837 59.187 30.832 1.00 13.26 N ATOM 1048 CA PRO X 143 13.386 57.916 31.331 1.00 12.95 C ATOM 1049 CB PRO X 143 14.883 58.028 31.019 1.00 13.14 C ATOM 1050 CG PRO X 143 14.968 59.082 29.927 1.00 13.92 C ATOM 1051 CD PRO X 143 13.896 60.060 30.299 1.00 13.17 C ATOM 1052 C PRO X 143 13.180 57.755 32.832 1.00 12.52 C ATOM 1053 O PRO X 143 13.219 58.741 33.574 1.00 11.53 O ATOM 1054 N THR X 144 12.981 56.516 33.278 1.00 12.24 N ATOM 1055 CA THR X 144 12.633 56.282 34.674 1.00 12.51 C ATOM 1056 CB THR X 144 11.169 55.837 34.837 1.00 12.44 C ATOM 1057 OG1 THR X 144 10.997 54.577 34.189 1.00 13.50 O ATOM 1058 CG2 THR X 144 10.219 56.844 34.220 1.00 12.05 C ATOM 1059 C THR X 144 13.506 55.239 35.342 1.00 12.57 C ATOM 1060 O THR X 144 14.054 54.340 34.686 1.00 13.12 O ATOM 1061 N ILE X 145 13.599 55.369 36.661 1.00 11.86 N ATOM 1062 CA ILE X 145 14.301 54.432 37.528 1.00 11.40 C ATOM 1063 CB ILE X 145 15.642 55.011 38.047 1.00 11.28 C ATOM 1064 CG1 ILE X 145 16.557 55.385 36.869 1.00 11.42 C ATOM 1065 CD1 ILE X 145 17.819 56.141 37.244 1.00 11.39 C ATOM 1066 CG2 ILE X 145 16.327 54.013 38.997 1.00 11.28 C ATOM 1067 C ILE X 145 13.366 54.206 38.698 1.00 11.06 C ATOM 1068 O ILE X 145 12.713 55.139 39.159 1.00 11.09 O ATOM 1069 N ALA X 146 13.276 52.970 39.171 1.00 10.74 N ATOM 1070 CA ALA X 146 12.411 52.690 40.308 1.00 10.92 C ATOM 1071 CB ALA X 146 11.000 52.297 39.848 1.00 9.62 C ATOM 1072 C ALA X 146 12.993 51.605 41.183 1.00 10.87 C ATOM 1073 O ALA X 146 13.660 50.699 40.701 1.00 10.77 O ATOM 1074 N LEU X 147 12.728 51.731 42.474 1.00 11.43 N ATOM 1075 CA LEU X 147 12.900 50.649 43.424 1.00 12.11 C ATOM 1076 CB LEU X 147 12.810 51.188 44.859 1.00 11.61 C ATOM 1077 CG LEU X 147 13.823 52.263 45.282 1.00 11.49 C ATOM 1078 CD1 LEU X 147 13.646 52.592 46.768 1.00 10.96 C ATOM 1079 CD2 LEU X 147 15.267 51.829 44.982 1.00 9.30 C ATOM 1080 C LEU X 147 11.772 49.663 43.163 1.00 12.57 C ATOM 1081 O LEU X 147 10.599 50.024 43.254 1.00 13.01 O ATOM 1082 N CYS X 148 12.120 48.425 42.834 1.00 13.23 N ATOM 1083 CA CYS X 148 11.120 47.457 42.398 1.00 13.55 C ATOM 1084 CB CYS X 148 11.357 47.064 40.941 1.00 13.85 C ATOM 1085 SG CYS X 148 11.109 48.395 39.766 1.00 14.86 S ATOM 1086 C CYS X 148 11.107 46.210 43.260 1.00 13.86 C ATOM 1087 O CYS X 148 12.135 45.536 43.418 1.00 14.09 O ATOM 1088 N ASN X 149 9.933 45.910 43.803 1.00 13.71 N ATOM 1089 CA ASN X 149 9.699 44.676 44.532 1.00 14.37 C ATOM 1090 CB ASN X 149 8.567 44.863 45.555 1.00 13.99 C ATOM 1091 CG ASN X 149 8.790 44.056 46.828 1.00 14.12 C ATOM 1092 OD1 ASN X 149 9.282 42.930 46.781 1.00 13.09 O ATOM 1093 ND2 ASN X 149 8.426 44.629 47.968 1.00 13.05 N ATOM 1094 C ASN X 149 9.376 43.545 43.551 1.00 14.94 C ATOM 1095 O ASN X 149 9.238 43.784 42.346 1.00 14.75 O ATOM 1096 N THR X 150 9.249 42.320 44.058 1.00 15.51 N ATOM 1097 CA THR X 150 9.016 41.159 43.183 1.00 16.22 C ATOM 1098 CB THR X 150 9.097 39.818 43.953 1.00 16.08 C ATOM 1099 OG1 THR X 150 8.102 39.796 44.982 1.00 15.68 O ATOM 1100 CG2 THR X 150 10.477 39.646 44.563 1.00 15.55 C ATOM 1101 C THR X 150 7.701 41.231 42.405 1.00 16.74 C ATOM 1102 O THR X 150 7.619 40.733 41.286 1.00 16.41 O ATOM 1103 N ASP X 151 6.687 41.859 42.995 1.00 17.93 N ATOM 1104 CA ASP X 151 5.398 42.032 42.328 1.00 19.59 C ATOM 1105 CB ASP X 151 4.254 41.705 43.288 1.00 20.07 C ATOM 1106 CG ASP X 151 4.158 42.690 44.438 1.00 22.98 C ATOM 1107 OD1 ASP X 151 3.104 42.715 45.116 1.00 27.10 O ATOM 1108 OD2 ASP X 151 5.135 43.429 44.686 1.00 25.50 O ATOM 1109 C ASP X 151 5.193 43.436 41.745 1.00 19.86 C ATOM 1110 O ASP X 151 4.067 43.797 41.381 1.00 20.26 O ATOM 1111 N SER X 152 6.264 44.226 41.668 1.00 19.95 N ATOM 1112 CA SER X 152 6.185 45.559 41.063 1.00 19.88 C ATOM 1113 CB SER X 152 7.465 46.361 41.299 1.00 19.46 C ATOM 1114 OG SER X 152 7.505 46.882 42.616 1.00 19.58 O ATOM 1115 C SER X 152 5.935 45.431 39.575 1.00 19.99 C ATOM 1116 O SER X 152 6.546 44.589 38.916 1.00 20.27 O ATOM 1117 N PRO X 153 5.019 46.253 39.038 1.00 20.10 N ATOM 1118 CA PRO X 153 4.888 46.328 37.583 1.00 20.13 C ATOM 1119 CB PRO X 153 3.572 47.095 37.397 1.00 20.02 C ATOM 1120 CG PRO X 153 3.467 47.960 38.620 1.00 20.52 C ATOM 1121 CD PRO X 153 4.060 47.132 39.737 1.00 20.09 C ATOM 1122 C PRO X 153 6.068 47.100 36.974 1.00 20.13 C ATOM 1123 O PRO X 153 6.397 48.201 37.442 1.00 20.58 O ATOM 1124 N LEU X 154 6.701 46.527 35.953 1.00 19.88 N ATOM 1125 CA LEU X 154 7.877 47.140 35.340 1.00 19.68 C ATOM 1126 CB LEU X 154 9.006 46.116 35.137 1.00 19.67 C ATOM 1127 CG LEU X 154 9.708 45.441 36.325 1.00 19.69 C ATOM 1128 CD1 LEU X 154 11.099 44.947 35.932 1.00 18.97 C ATOM 1129 CD2 LEU X 154 9.814 46.371 37.505 1.00 19.52 C ATOM 1130 C LEU X 154 7.556 47.822 34.016 1.00 19.52 C ATOM 1131 O LEU X 154 8.455 48.298 33.324 1.00 19.51 O ATOM 1132 N ARG X 155 6.273 47.855 33.669 1.00 19.21 N ATOM 1133 CA ARG X 155 5.807 48.440 32.414 1.00 18.99 C ATOM 1134 CB ARG X 155 4.280 48.525 32.420 1.00 19.06 C ATOM 1135 CG ARG X 155 3.690 49.105 31.143 1.00 20.28 C ATOM 1136 CD ARG X 155 2.234 48.738 31.015 1.00 22.06 C ATOM 1137 NE ARG X 155 2.086 47.302 30.790 1.00 22.67 N ATOM 1138 CZ ARG X 155 0.966 46.619 30.985 1.00 23.37 C ATOM 1139 NH1 ARG X 155 −0.130 47.229 31.415 1.00 23.18 N ATOM 1140 NH2 ARG X 155 0.951 45.310 30.758 1.00 24.79 N ATOM 1141 C ARG X 155 6.409 49.820 32.100 1.00 18.45 C ATOM 1142 O ARG X 155 6.827 50.076 30.968 1.00 18.62 O ATOM 1143 N TYR X 156 6.446 50.693 33.106 1.00 17.73 N ATOM 1144 CA TYR X 156 6.878 52.085 32.939 1.00 16.79 C ATOM 1145 CB TYR X 156 5.847 53.023 33.571 1.00 16.48 C ATOM 1146 CG TYR X 156 4.451 52.845 33.010 1.00 16.91 C ATOM 1147 CD1 TYR X 156 4.178 53.106 31.666 1.00 16.32 C ATOM 1148 CE1 TYR X 156 2.898 52.940 31.144 1.00 15.84 C ATOM 1149 CZ TYR X 156 1.878 52.504 31.965 1.00 16.74 C ATOM 1150 OH TYR X 156 0.610 52.348 31.456 1.00 16.88 O ATOM 1151 CE2 TYR X 156 2.120 52.230 33.302 1.00 17.26 C ATOM 1152 CD2 TYR X 156 3.405 52.402 33.818 1.00 17.69 C ATOM 1153 C TYR X 156 8.264 52.348 33.526 1.00 16.33 C ATOM 1154 O TYR X 156 8.699 53.504 33.631 1.00 16.24 O ATOM 1155 N VAL X 157 8.951 51.273 33.896 1.00 15.40 N ATOM 1156 CA VAL X 157 10.264 51.363 34.529 1.00 15.08 C ATOM 1157 CB VAL X 157 10.365 50.408 35.768 1.00 14.94 C ATOM 1158 CG1 VAL X 157 11.780 50.436 36.362 1.00 14.49 C ATOM 1159 CG2 VAL X 157 9.324 50.769 36.830 1.00 13.72 C ATOM 1160 C VAL X 157 11.342 51.001 33.505 1.00 15.43 C ATOM 1161 O VAL X 157 11.306 49.903 32.942 1.00 15.27 O ATOM 1162 N ASP X 158 12.286 51.918 33.268 1.00 15.20 N ATOM 1163 CA ASP X 158 13.423 51.656 32.372 1.00 15.42 C ATOM 1164 CB ASP X 158 13.912 52.946 31.685 1.00 15.78 C ATOM 1165 CG ASP X 158 12.840 53.602 30.811 1.00 17.28 C ATOM 1166 OD1 ASP X 158 12.315 52.959 29.869 1.00 18.93 O ATOM 1167 OD2 ASP X 158 12.530 54.780 31.057 1.00 18.99 O ATOM 1168 C ASP X 158 14.590 50.973 33.106 1.00 15.10 C ATOM 1169 O ASP X 158 15.176 50.005 32.598 1.00 15.16 O ATOM 1170 N ILE X 159 14.942 51.488 34.282 1.00 14.23 N ATOM 1171 CA ILE X 159 15.939 50.838 35.125 1.00 13.89 C ATOM 1172 CB ILE X 159 17.174 51.732 35.426 1.00 14.16 C ATOM 1173 CG1 ILE X 159 17.942 52.079 34.139 1.00 13.81 C ATOM 1174 CD1 ILE X 159 18.986 53.186 34.338 1.00 13.96 C ATOM 1175 CG2 ILE X 159 18.101 51.034 36.446 1.00 13.94 C ATOM 1176 C ILE X 159 15.285 50.416 36.432 1.00 13.63 C ATOM 1177 O ILE X 159 14.884 51.267 37.238 1.00 13.47 O ATOM 1178 N ALA X 160 15.168 49.102 36.618 1.00 13.06 N ATOM 1179 CA ALA X 160 14.592 48.513 37.828 1.00 13.00 C ATOM 1180 CB ALA X 160 13.784 47.269 37.476 1.00 12.71 C ATOM 1181 C ALA X 160 15.687 48.159 38.822 1.00 12.72 C ATOM 1182 O ALA X 160 16.635 47.448 38.480 1.00 13.40 O ATOM 1183 N ILE X 161 15.566 48.673 40.040 1.00 12.22 N ATOM 1184 CA ILE X 161 16.452 48.295 41.126 1.00 12.07 C ATOM 1185 CB ILE X 161 16.933 49.525 41.952 1.00 11.98 C ATOM 1186 CG1 ILE X 161 17.521 50.607 41.038 1.00 11.82 C ATOM 1187 CD1 ILE X 161 17.733 51.946 41.727 1.00 11.76 C ATOM 1188 CG2 ILE X 161 17.979 49.097 42.968 1.00 12.04 C ATOM 1189 C ILE X 161 15.679 47.310 42.024 1.00 12.00 C ATOM 1190 O ILE X 161 14.774 47.719 42.749 1.00 11.80 O ATOM 1191 N PRO X 162 16.018 46.008 41.955 1.00 12.25 N ATOM 1192 CA PRO X 162 15.244 45.021 42.712 1.00 12.11 C ATOM 1193 CB PRO X 162 15.699 43.686 42.121 1.00 12.20 C ATOM 1194 CG PRO X 162 17.072 43.946 41.591 1.00 11.87 C ATOM 1195 CD PRO X 162 17.107 45.385 41.175 1.00 12.06 C ATOM 1196 C PRO X 162 15.544 45.099 44.201 1.00 12.22 C ATOM 1197 O PRO X 162 16.711 45.093 44.597 1.00 12.32 O ATOM 1198 N CYS X 163 14.495 45.202 45.013 1.00 12.31 N ATOM 1199 CA CYS X 163 14.650 45.339 46.455 1.00 13.05 C ATOM 1200 CB CYS X 163 15.315 46.685 46.787 1.00 13.19 C ATOM 1201 SG CYS X 163 14.357 48.091 46.189 1.00 14.37 S ATOM 1202 C CYS X 163 13.302 45.258 47.166 1.00 13.21 C ATOM 1203 O CYS X 163 12.244 45.352 46.532 1.00 12.99 O ATOM 1204 N ASN X 164 13.355 45.070 48.484 1.00 13.29 N ATOM 1205 CA ASN X 164 12.220 45.353 49.355 1.00 13.54 C ATOM 1206 CB ASN X 164 12.531 44.883 50.783 1.00 13.28 C ATOM 1207 CG ASN X 164 11.386 45.130 51.769 1.00 13.91 C ATOM 1208 OD1 ASN X 164 10.380 45.756 51.444 1.00 14.80 O ATOM 1209 ND2 ASN X 164 11.553 44.638 52.994 1.00 13.95 N ATOM 1210 C ASN X 164 11.987 46.871 49.319 1.00 13.94 C ATOM 1211 O ASN X 164 12.741 47.640 49.926 1.00 14.14 O ATOM 1212 N ASN X 165 10.970 47.293 48.579 1.00 14.20 N ATOM 1213 CA ASN X 165 10.604 48.701 48.514 1.00 15.18 C ATOM 1214 CB ASN X 165 10.377 49.144 47.059 1.00 14.77 C ATOM 1215 CG ASN X 165 9.096 48.581 46.462 1.00 15.34 C ATOM 1216 OD1 ASN X 165 8.433 47.737 47.061 1.00 17.22 O ATOM 1217 ND2 ASN X 165 8.741 49.052 45.274 1.00 16.27 N ATOM 1218 C ASN X 165 9.360 48.988 49.364 1.00 15.88 C ATOM 1219 O ASN X 165 8.702 50.011 49.175 1.00 16.07 O ATOM 1220 N LYS X 166 9.049 48.074 50.284 1.00 16.53 N ATOM 1221 CA LYS X 166 7.898 48.202 51.169 1.00 17.51 C ATOM 1222 CB LYS X 166 7.095 46.896 51.176 1.00 17.80 C ATOM 1223 CG LYS X 166 5.864 46.935 52.080 1.00 21.27 C ATOM 1224 CD LYS X 166 5.281 45.548 52.301 1.00 26.08 C ATOM 1225 CE LYS K 166 4.165 45.584 53.340 1.00 29.90 C ATOM 1226 NZ LYS X 166 3.050 46.503 52.943 1.00 32.28 N ATOM 1227 C LYS X 166 8.302 48.596 52.599 1.00 17.32 C ATOM 1228 O LYS X 166 7.784 49.564 53.156 1.00 17.63 O ATOM 1229 N GLY X 167 9.226 47.846 53.190 1.00 17.29 N ATOM 1230 CA GLY X 167 9.652 48.098 54.569 1.00 16.94 C ATOM 1231 C GLY X 167 10.373 49.424 54.690 1.00 16.73 C ATOM 1232 O GLY X 167 11.108 49.820 53.782 1.00 16.04 O ATOM 1233 N ALA X 168 10.168 50.102 55.820 1.00 16.45 N ATOM 1234 CA ALA X 168 10.729 51.437 56.050 1.00 16.34 C ATOM 1235 CB ALA X 168 10.155 52.050 57.339 1.00 16.64 C ATOM 1236 C ALA X 168 12.257 51.488 56.068 1.00 16.30 C ATOM 1237 O ALA X 168 12.858 52.397 55.483 1.00 15.90 O ATOM 1238 N HIS X 169 12.886 50.522 56.738 1.00 16.23 N ATOM 1239 CA HIS X 169 14.350 50.485 56.818 1.00 16.44 C ATOM 1240 CB HIS X 169 14.823 49.424 57.812 1.00 17.20 C ATOM 1241 CG HIS X 169 14.662 49.818 59.248 1.00 20.89 C ATOM 1242 ND1 HIS X 169 15.450 49.296 60.250 1.00 24.67 N ATOM 1243 CE1 HIS X 169 15.084 49.814 61.411 1.00 25.88 C ATOM 1244 NE2 HIS X 169 14.091 50.658 61.197 1.00 25.28 N ATOM 1245 CD2 HIS X 169 13.809 50.681 59.852 1.00 23.96 C ATOM 1246 C HIS X 169 14.983 50.240 55.451 1.00 15.63 C ATOM 1247 O HIS X 169 15.973 50.882 55.096 1.00 15.48 O ATOM 1248 N SER X 170 14.398 49.320 54.692 1.00 14.82 N ATOM 1249 CA SER X 170 14.895 48.982 53.360 1.00 14.35 C ATOM 1250 CB SER X 170 14.211 47.724 52.827 1.00 14.25 C ATOM 1251 OG SER X 170 14.739 47.364 51.560 1.00 15.22 O ATOM 1252 C SER X 170 14.737 50.145 52.381 1.00 13.75 C ATOM 1253 O SER X 170 15.668 50.455 51.647 1.00 13.52 O ATOM 1254 N VAL X 171 13.569 50.788 52.375 1.00 13.45 N ATOM 1255 CA VAL X 171 13.360 51.973 51.523 1.00 13.20 C ATOM 1256 CB VAL X 171 11.933 52.544 51.640 1.00 12.91 C ATOM 1257 CG1 VAL X 171 11.823 53.920 50.950 1.00 13.68 C ATOM 1258 CG2 VAL X 171 10.926 51.581 51.061 1.00 13.64 C ATOM 1259 C VAL X 171 14.386 53.057 51.850 1.00 12.85 C ATOM 1260 O VAL X 171 15.026 53.605 50.951 1.00 12.45 O ATOM 1261 N GLY X 172 14.542 53.356 53.137 1.00 12.67 N ATOM 1262 CA GLY X 172 15.489 54.386 53.577 1.00 12.48 C ATOM 1263 C GLY X 172 16.921 54.052 53.199 1.00 12.33 C ATOM 1264 O GLY X 172 17.664 54.914 52.719 1.00 12.19 O ATOM 1265 N LEU X 173 17.297 52.792 53.402 1.00 12.45 N ATOM 1266 CA LEU X 173 18.639 52.313 53.075 1.00 12.79 C ATOM 1267 CB LEU X 173 18.847 50.875 53.560 1.00 12.76 C ATOM 1268 CG LEU X 173 20.166 50.183 53.164 1.00 13.27 C ATOM 1269 CD1 LEU X 173 21.357 50.818 53.885 1.00 13.69 C ATOM 1270 CD2 LEU X 173 20.104 48.675 53.440 1.00 12.89 C ATOM 1271 C LEU X 173 18.918 52.397 51.579 1.00 12.76 C ATOM 1272 O LEU X 173 20.017 52.778 51.176 1.00 13.12 O ATOM 1273 N MET X 174 17.929 52.034 50.763 1.00 12.59 N ATOM 1274 CA MET X 174 18.102 52.059 49.308 1.00 12.69 C ATOM 1275 CB MET X 174 16.875 51.479 48.591 1.00 12.53 C ATOM 1276 CG MET X 174 16.734 49.947 48.706 1.00 12.61 C ATOM 1277 SD MET X 174 18.170 48.973 48.177 1.00 13.33 S ATOM 1278 CE MET X 174 18.237 49.361 46.425 1.00 11.52 C ATOM 1279 C MET X 174 18.421 53.471 48.809 1.00 12.66 C ATOM 1280 O MET X 174 19.379 53.667 48.048 1.00 12.36 O ATOM 1281 N TRP X 175 17.628 54.446 49.258 1.00 12.66 N ATOM 1282 CA TRP X 175 17.836 55.850 48.896 1.00 13.00 C ATOM 1283 CB TRP X 175 16.670 56.711 49.395 1.00 12.45 C ATOM 1284 CG TRP X 175 15.474 56.665 48.486 1.00 11.86 C ATOM 1285 CD1 TRP X 175 14.280 56.046 48.722 1.00 10.69 C ATOM 1286 NE1 TRP X 175 13.431 56.225 47.653 1.00 10.74 N ATOM 1287 CE2 TRP X 175 14.076 56.970 46.698 1.00 11.46 C ATOM 1288 CD2 TRP X 175 15.365 57.269 47.193 1.00 11.64 C ATOM 1289 CE3 TRP X 175 16.240 58.027 46.397 1.00 11.84 C ATOM 1290 CZ3 TRP X 175 15.796 58.474 45.158 1.00 11.34 C ATOM 1291 CH2 TRP X 175 14.500 58.164 44.696 1.00 11.34 C ATOM 1292 CZ2 TRP X 175 13.632 57.413 45.444 1.00 11.62 C ATOM 1293 C TRP X 175 19.175 56.379 49.412 1.00 13.57 C ATOM 1294 O TRP X 175 19.878 57.098 48.706 1.00 14.02 O ATOM 1295 N TRP X 176 19.527 55.998 50.638 1.00 14.79 N ATOM 1296 CA TRP X 176 20.838 56.320 51.223 1.00 15.57 C ATOM 1297 CB TRP X 176 20.904 55.819 52.677 1.00 15.95 C ATOM 1298 CG TRP X 176 22.234 56.003 53.351 1.00 16.75 C ATOM 1299 CD1 TRP X 176 22.640 57.084 54.089 1.00 17.25 C ATOM 1300 NE1 TRP X 176 23.924 56.886 54.551 1.00 16.59 N ATOM 1301 CE2 TRP X 176 24.369 55.664 54.121 1.00 16.79 C ATOM 1302 CD2 TRP X 176 23.326 55.073 53.365 1.00 17.27 C ATOM 1303 CE3 TRP X 176 23.528 53.798 52.811 1.00 16.82 C ATOM 1304 CZ3 TRP X 176 24.752 53.159 53.025 1.00 16.49 C ATOM 1305 CH2 TRP X 176 25.768 53.775 53.785 1.00 17.43 C ATOM 1306 CZ2 TRP X 176 25.595 55.022 54.339 1.00 17.22 C ATOM 1307 C TRP X 176 21.986 55.745 50.385 1.00 15.71 C ATOM 1308 O TRP X 176 22.953 56.446 50.069 1.00 15.13 O ATOM 1309 N MET X 177 21.867 54.474 50.008 1.00 16.31 N ATOM 1310 CA MET X 177 22.900 53.831 49.199 1.00 17.65 C ATOM 1311 CB MET X 177 22.677 52.329 49.130 1.00 17.44 C ATOM 1312 CG MET X 177 23.975 51.559 49.074 1.00 19.87 C ATOM 1313 SD MET X 177 23.742 49.791 49.294 1.00 22.04 S ATOM 1314 CE MET X 177 23.557 49.637 51.067 1.00 21.57 C ATOM 1315 C MET X 177 23.013 54.425 47.791 1.00 16.68 C ATOM 1316 O MET X 177 24.119 54.573 47.263 1.00 16.13 O ATOM 1317 N LEU X 178 21.866 54.755 47.196 1.00 16.39 N ATOM 1318 CA LEU X 178 21.831 55.439 45.905 1.00 16.64 C ATOM 1319 CB LEU X 178 20.392 55.740 45.469 1.00 16.45 C ATOM 1320 CG LEU X 178 19.930 55.495 44.020 1.00 18.25 C ATOM 1321 CD1 LEU X 178 18.855 56.495 43.575 1.00 16.38 C ATOM 1322 CD2 LEU X 178 21.057 55.389 42.991 1.00 16.98 C ATOM 1323 C LEU X 178 22.603 56.747 46.016 1.00 16.16 C ATOM 1324 O LEU X 178 23.482 57.007 45.208 1.00 16.08 O ATOM 1325 N ALA X 179 22.265 57.548 47.028 1.00 16.24 N ATOM 1326 CA ALA X 179 22.939 58.832 47.300 1.00 16.51 C ATOM 1327 CB ALA X 179 22.335 59.510 48.542 1.00 16.39 C ATOM 1328 C ALA X 179 24.443 58.678 47.460 1.00 16.51 C ATOM 1329 O ALA X 179 25.215 59.402 46.829 1.00 16.60 O ATOM 1330 N ARG X 180 24.855 57.726 48.297 1.00 16.59 N ATOM 1331 CA ARG X 180 26.277 57.453 48.526 1.00 16.53 C ATOM 1332 CB ARG X 180 26.437 56.270 49.490 1.00 16.40 C ATOM 1333 CG ARG X 180 27.846 56.088 50.056 1.00 16.13 C ATOM 1334 CD ARG X 180 27.952 54.790 50.850 1.00 16.00 C ATOM 1335 NE ARG X 180 27.730 53.637 49.988 1.00 17.01 N ATOM 1336 CZ ARG X 180 27.668 52.370 50.395 1.00 18.18 C ATOM 1337 NH1 ARG X 180 27.817 52.052 51.675 1.00 19.33 N ATOM 1338 NH2 ARG X 180 27.467 51.413 49.507 1.00 18.03 N ATOM 1339 C ARG X 180 27.026 57.170 47.221 1.00 16.69 C ATOM 1340 O ARG X 180 28.132 57.681 47.008 1.00 17.18 O ATOM 1341 N GLU X 181 26.423 56.357 46.353 1.00 16.97 N ATOM 1342 CA GLU X 181 27.056 55.970 45.090 1.00 17.18 C ATOM 1343 CB GLU X 181 26.392 54.725 44.483 1.00 16.83 C ATOM 1344 CG GLU X 181 26.595 53.443 45.306 1.00 19.23 C ATOM 1345 CD GLU X 181 28.046 53.213 45.726 1.00 19.32 C ATOM 1346 OE1 GLU X 181 28.947 53.223 44.862 1.00 20.23 O ATOM 1347 OE2 GLU X 181 28.280 53.009 46.926 1.00 21.36 O ATOM 1348 C GLU X 181 27.083 57.106 44.072 1.00 16.79 C ATOM 1349 O GLU X 181 28.018 57.200 43.282 1.00 16.37 O ATOM 1350 N VAL X 182 26.053 57.948 44.081 1.00 16.86 N ATOM 1351 CA VAL X 182 26.052 59.152 43.238 1.00 17.35 C ATOM 1352 CB VAL X 182 24.693 59.895 43.286 1.00 17.24 C ATOM 1353 CG1 VAL X 182 24.818 61.300 42.706 1.00 17.18 C ATOM 1354 CG2 VAL X 182 23.620 59.096 42.539 1.00 17.12 C ATOM 1355 C VAL X 182 27.208 60.069 43.660 1.00 17.37 C ATOM 1356 O VAL X 182 28.003 60.501 42.827 1.00 16.87 O ATOM 1357 N LEU X 183 27.306 60.326 44.961 1.00 18.04 N ATOM 1358 CA LEU X 183 28.365 61.177 45.520 1.00 19.17 C ATOM 1359 CB LEU X 183 28.119 61.446 47.008 1.00 18.96 C ATOM 1360 CG LEU X 183 26.871 62.282 47.331 1.00 19.49 C ATOM 1361 CD1 LEU X 183 26.695 62.445 48.833 1.00 19.23 C ATOM 1362 CD2 LEU X 183 26.911 63.650 46.633 1.00 19.34 C ATOM 1363 C LEU X 183 29.766 60.610 45.307 1.00 19.85 C ATOM 1364 O LEU X 183 30.712 61.366 45.088 1.00 20.20 O ATOM 1365 N ARG X 184 29.895 59.286 45.379 1.00 20.45 N ATOM 1366 CA ARG X 184 31.168 58.630 45.093 1.00 21.70 C ATOM 1367 CB ARG X 184 31.134 57.155 45.504 1.00 21.78 C ATOM 1368 CG ARG X 184 31.334 56.918 46.995 1.00 22.18 C ATOM 1369 CD ARG X 184 31.159 55.442 47.339 1.00 22.57 C ATOM 1370 NE ARG X 184 31.547 55.136 48.715 1.00 23.68 N ATOM 1371 CZ ARG X 184 31.359 53.957 49.306 1.00 25.29 C ATOM 1372 NH1 ARG X 184 30.769 52.958 48.651 1.00 25.15 N ATOM 1373 NH2 ARG X 184 31.755 53.779 50.561 1.00 26.01 N ATOM 1374 C ARG X 184 31.567 58.764 43.620 1.00 22.01 C ATOM 1375 O ARG X 184 32.728 59.025 43.308 1.00 22.30 O ATOM 1376 N MET X 185 30.607 58.588 42.719 1.00 22.36 N ATOM 1377 CA MET X 185 30.887 58.685 41.288 1.00 23.07 C ATOM 1378 CB MET X 185 29.689 58.212 40.468 1.00 22.89 C ATOM 1379 CG MET X 185 29.402 56.735 40.640 1.00 23.31 C ATOM 1380 SD MET X 185 28.182 56.149 39.465 1.00 23.73 S ATOM 1381 CE MET X 185 28.238 54.384 39.799 1.00 22.03 C ATOM 1382 C MET X 185 31.304 60.098 40.886 1.00 23.37 C ATOM 1383 O MET X 185 32.194 60.273 40.052 1.00 23.40 O ATOM 1384 N ARG X 186 30.674 61.091 41.511 1.00 23.70 N ATOM 1385 CA ARG X 186 31.020 62.503 41.327 1.00 24.39 C ATOM 1386 CB ARG X 186 29.917 63.390 41.908 1.00 23.94 C ATOM 1387 CG ARG X 186 28.626 63.399 41.099 1.00 22.89 C ATOM 1388 CD ARG X 186 27.633 64.341 41.727 1.00 20.94 C ATOM 1389 NE ARG X 186 26.332 64.337 41.062 1.00 19.69 N ATOM 1390 CZ ARG X 186 25.212 64.781 41.627 1.00 17.82 C ATOM 1391 NH1 ARG X 186 25.233 65.239 42.874 1.00 17.83 N ATOM 1392 NH2 ARG X 186 24.067 64.749 40.959 1.00 15.96 N ATOM 1393 C ARG X 186 32.364 62.899 41.953 1.00 25.26 C ATOM 1394 O ARG X 186 32.916 63.951 41.625 1.00 25.21 O ATOM 1395 N GLY X 187 32.873 62.061 42.855 1.00 26.07 N ATOM 1396 CA GLY X 187 34.102 62.350 43.589 1.00 27.26 C ATOM 1397 C GLY X 187 33.860 63.316 44.730 1.00 28.25 C ATOM 1398 O GLY X 187 34.797 63.950 45.216 1.00 28.55 O ATOM 1399 N THR X 188 32.599 63.435 45.147 1.00 28.80 N ATOM 1400 CA THR X 188 32.201 64.330 46.230 1.00 29.74 C ATOM 1401 CB THR X 188 30.675 64.593 46.200 1.00 29.52 C ATOM 1402 OG1 THR X 188 30.307 65.134 44.924 1.00 29.63 O ATOM 1403 CG2 THR X 188 30.250 65.564 47.302 1.00 29.67 C ATOM 1404 C THR X 188 32.608 63.757 47.594 1.00 30.65 C ATOM 1405 O THR X 188 32.975 64.505 48.505 1.00 30.74 O ATOM 1406 N ILE X 189 32.520 62.434 47.726 1.00 31.59 N ATOM 1407 CA ILE X 189 32.952 61.731 48.931 1.00 32.51 C ATOM 1408 CB ILE X 189 31.761 61.166 49.167 1.00 32.45 C ATOM 1409 CG1 ILE X 189 31.003 60.074 48.991 1.00 31.89 C ATOM 1410 CD1 ILE X 189 29.990 59.295 49.824 1.00 32.04 C ATOM 1411 CG2 ILE X 189 30.842 62.298 50.244 1.00 32.02 C ATOM 1412 C ILE X 189 33.905 60.600 48.565 1.00 33.74 C ATOM 1413 O ILE X 189 33.996 60.200 47.401 1.00 33.49 O ATOM 1414 N SER X 190 34.603 60.088 49.573 1.00 35.56 N ATOM 1415 CA SER X 190 35.582 59.020 49.393 1.00 37.44 C ATOM 1416 CB SER X 190 36.366 58.788 50.685 1.00 37.26 C ATOM 1417 OG SER X 190 37.180 57.630 50.578 1.00 38.17 O ATOM 1418 C SER X 190 34.957 57.707 48.924 1.00 38.68 C ATOM 1419 O SER X 190 33.913 57.274 49.425 1.00 38.89 O ATOM 1420 N ARG X 191 35.636 57.078 47.972 1.00 40.35 N ATOM 1421 CA ARG X 191 35.216 55.820 47.375 1.00 42.05 C ATOM 1422 CB ARG X 191 35.837 55.712 45.981 1.00 42.21 C ATOM 1423 CG ARG X 191 34.941 55.114 44.924 1.00 43.86 C ATOM 1424 CD ARG X 191 35.428 55.482 43.524 1.00 46.98 C ATOM 1425 NE ARG X 191 35.514 56.931 43.320 1.00 48.37 N ATOM 1426 CZ ARG X 191 35.511 57.530 42.130 1.00 49.47 C ATOM 1427 NH1 ARG X 191 35.419 56.812 41.016 1.00 49.92 N ATOM 1428 NH2 ARG X 191 35.593 58.854 42.052 1.00 49.66 N ATOM 1429 C ARG X 191 35.660 54.631 48.235 1.00 42.95 C ATOM 1430 O ARG X 191 35.143 53.517 48.087 1.00 43.12 O ATOM 1431 N GLU X 192 36.612 54.876 49.136 1.00 44.02 N ATOM 1432 CA GLU X 192 37.237 53.805 49.920 1.00 45.03 C ATOM 1433 CB GLU X 192 38.759 53.997 49.991 1.00 45.27 C ATOM 1434 CG GLU X 192 39.514 53.573 48.722 1.00 46.86 C ATOM 1435 CD GLU X 192 39.505 54.633 47.619 1.00 49.21 C ATOM 1436 OE1 GLU X 192 39.390 55.841 47.937 1.00 50.44 O ATOM 1437 OE2 GLU X 192 39.622 54.257 46.429 1.00 49.88 O ATOM 1438 C GLU X 192 36.641 53.590 51.320 1.00 45.20 C ATOM 1439 O GLU X 192 36.235 52.469 51.649 1.00 45.52 O ATOM 1440 N HIS X 193 36.589 54.647 52.136 1.00 45.32 N ATOM 1441 CA HIS X 193 36.067 54.530 53.508 1.00 45.33 C ATOM 1442 CB HIS X 193 36.903 55.343 54.519 1.00 45.76 C ATOM 1443 CG HIS X 193 36.728 56.830 54.423 1.00 47.14 C ATOM 1444 ND1 HIS X 193 35.675 57.498 55.014 1.00 48.11 N ATOM 1445 CE1 HIS X 193 35.788 58.794 54.777 1.00 48.61 C ATOM 1446 NE2 HIS X 193 36.886 58.994 54.069 1.00 48.65 N ATOM 1447 CD2 HIS X 193 37.496 57.783 53.841 1.00 48.49 C ATOM 1448 C HIS X 193 34.567 54.847 53.617 1.00 44.88 C ATOM 1449 O HIS X 193 34.028 55.576 52.779 1.00 44.95 O ATOM 1450 N PRO X 194 33.887 54.274 54.636 1.00 44.30 N ATOM 1451 CA PRO X 194 32.461 54.508 54.881 1.00 43.71 C ATOM 1452 CB PRO X 194 32.249 53.908 56.272 1.00 43.81 C ATOM 1453 CG PRO X 194 33.240 52.809 56.343 1.00 44.02 C ATOM 1454 CD PRO X 194 34.451 53.324 55.618 1.00 44.29 C ATOM 1455 C PRO X 194 32.061 55.984 54.885 1.00 43.07 C ATOM 1456 O PRO X 194 32.853 56.846 55.277 1.00 42.96 O ATOM 1457 N TRP X 195 30.830 56.245 54.451 1.00 42.19 N ATOM 1458 CA TRP X 195 30.264 57.590 54.380 1.00 41.46 C ATOM 1459 CB TRP X 195 29.054 57.583 53.433 1.00 40.37 C ATOM 1460 CG TRP X 195 28.438 58.928 53.110 1.00 39.32 C ATOM 1461 CD1 TRP X 195 29.039 60.156 53.173 1.00 38.23 C ATOM 1462 NE1 TRP X 195 28.158 61.139 52.781 1.00 37.52 N ATOM 1463 CE2 TRP X 195 26.967 60.557 52.435 1.00 37.76 C ATOM 1464 CD2 TRP X 195 27.107 59.162 52.619 1.00 38.28 C ATOM 1465 CE3 TRP X 195 26.015 58.329 52.333 1.00 37.46 C ATOM 1466 CZ3 TRP X 195 24.833 58.907 51.875 1.00 37.85 C ATOM 1467 CH2 TRP X 195 24.728 60.297 51.701 1.00 38.37 C ATOM 1468 CZ2 TRP X 195 25.780 61.137 51.975 1.00 38.03 C ATOM 1469 C TRP X 195 29.864 58.091 55.767 1.00 41.67 C ATOM 1470 O TRP X 195 29.347 57.324 56.584 1.00 41.85 O ATOM 1471 N GLU X 196 30.110 59.381 56.007 1.00 41.79 N ATOM 1472 CA GLU X 196 29.788 60.068 57.265 1.00 41.88 C ATOM 1473 CB GLU X 196 30.214 61.548 57.171 1.00 42.20 C ATOM 1474 CG GLU X 196 29.283 62.434 56.318 1.00 43.93 C ATOM 1475 CD GLU X 196 30.009 63.428 55.412 1.00 45.98 C ATOM 1476 OE1 GLU X 196 29.649 64.627 55.450 1.00 46.79 O ATOM 1477 OE2 GLU X 196 30.917 63.018 54.650 1.00 46.73 O ATOM 1478 C GLU X 196 28.308 59.950 57.673 1.00 41.33 C ATOM 1479 O GLU X 196 27.996 59.714 58.843 1.00 41.42 O ATOM 1480 N VAL X 197 27.414 60.110 56.697 1.00 40.67 N ATOM 1481 CA VAL X 197 25.964 60.085 56.908 1.00 39.71 C ATOM 1482 CB VAL X 197 25.214 60.688 55.683 1.00 39.76 C ATOM 1483 CG1 VAL X 197 23.701 60.599 55.846 1.00 39.02 C ATOM 1484 CG2 VAL X 197 25.642 62.136 55.458 1.00 39.49 C ATOM 1485 C VAL X 197 25.484 58.661 57.190 1.00 39.35 C ATOM 1486 O VAL X 198 25.827 57.727 56.462 1.00 39.19 O ATOM 1487 N MET X 198 24.710 58.510 58.264 1.00 38.79 N ATOM 1488 CA MET X 198 24.122 57.228 58.643 1.00 38.71 C ATOM 1489 CB MET X 198 23.902 57.156 60.157 1.00 38.67 C ATOM 1490 CG MET X 198 25.182 57.054 60.988 1.00 40.04 C ATOM 1491 SD MET X 198 24.856 56.740 62.744 1.00 42.11 S ATOM 1492 CE MET X 198 23.934 58.216 63.213 1.00 41.75 C ATOM 1493 C MET X 198 22.794 56.993 57.923 1.00 37.14 C ATOM 1494 O MET X 198 22.029 57.938 57.715 1.00 36.78 O ATOM 1495 N PRO X 199 22.521 55.731 57.531 1.00 36.06 N ATOM 1496 CA PRO X 199 21.247 55.353 56.915 1.00 35.29 C ATOM 1497 CB PRO X 199 21.331 53.825 56.857 1.00 35.14 C ATOM 1498 CG PRO X 199 22.786 53.549 56.744 1.00 35.18 C ATOM 1499 CD PRO X 199 23.446 54.583 57.606 1.00 35.80 C ATOM 1500 C PRO X 199 19.997 55.795 57.683 1.00 34.70 C ATOM 1501 O PRO X 199 19.013 56.175 57.053 1.00 34.06 O ATOM 1502 N ASP X 200 20.038 55.759 59.017 1.00 34.37 N ATOM 1503 CA ASP X 200 18.851 56.064 59.835 1.00 34.36 C ATOM 1504 CB ASP X 200 19.066 55.719 61.323 1.00 34.77 C ATOM 1505 CG ASP X 200 20.318 56.353 61.908 1.00 35.96 C ATOM 1506 OD1 ASP X 200 20.520 57.578 61.757 1.00 36.32 O ATOM 1507 OD2 ASP X 200 21.100 55.614 62.546 1.00 38.55 O ATOM 1508 C ASP X 200 18.294 57.484 59.661 1.00 33.81 C ATOM 1509 O ASP X 200 17.142 57.752 60.012 1.00 33.78 O ATOM 1510 N LEU X 201 19.107 58.379 59.104 1.00 33.23 N ATOM 1511 CA LEU X 201 18.654 59.721 58.732 1.00 32.90 C ATOM 1512 CB LEU X 201 19.855 60.602 58.351 1.00 33.14 C ATOM 1513 CG LEU X 201 20.646 61.383 59.424 1.00 33.94 C ATOM 1514 CD1 LEU X 201 20.907 60.605 60.718 1.00 34.33 C ATOM 1515 CD2 LEU X 201 21.965 61.886 58.857 1.00 33.61 C ATOM 1516 C LEU X 201 17.622 59.679 57.595 1.00 32.28 C ATOM 1517 O LEU X 201 16.834 60.608 57.430 1.00 32.29 O ATOM 1518 N TYR X 202 17.622 58.587 56.832 1.00 31.44 N ATOM 1519 CA TYR X 202 16.721 58.410 55.688 1.00 30.77 C ATOM 1520 CB TYR X 202 17.450 57.674 54.556 1.00 29.63 C ATOM 1521 CG TYR X 202 18.407 58.530 53.759 1.00 28.57 C ATOM 1522 CD1 TYR X 202 19.514 59.133 54.361 1.00 27.58 C ATOM 1523 CE1 TYR X 202 20.389 59.925 53.628 1.00 26.51 C ATOM 1524 CZ TYR X 202 20.170 60.107 52.273 1.00 27.26 C ATOM 1525 OH TYR X 202 21.036 60.880 51.539 1.00 27.46 O ATOM 1526 CE2 TYR X 202 19.087 59.516 51.650 1.00 26.92 C ATOM 1527 CD2 TYR X 202 18.212 58.732 52.392 1.00 27.09 C ATOM 1528 C TYR X 202 15.430 57.664 56.044 1.00 31.15 C ATOM 1529 O TYR X 202 14.497 57.616 55.235 1.00 30.65 O ATOM 1530 N PHE X 203 15.377 57.096 57.251 1.00 32.02 N ATOM 1531 CA PHE X 203 14.262 56.229 57.657 1.00 33.09 C ATOM 1532 CB PHE X 203 14.651 55.269 58.802 1.00 32.95 C ATOM 1533 CG PHE X 203 15.769 54.303 58.475 1.00 32.93 C ATOM 1534 CD1 PHE X 203 16.303 54.202 57.193 1.00 32.79 C ATOM 1535 CE1 PHE X 203 17.332 53.303 56.917 1.00 33.35 C ATOM 1536 CZ PHE X 203 17.828 52.472 57.930 1.00 33.35 C ATOM 1537 CE2 PHE X 203 17.300 52.556 59.206 1.00 33.16 C ATOM 1538 CD2 PHE X 203 16.271 53.465 59.472 1.00 33.62 C ATOM 1539 C PHE X 203 13.031 57.012 58.096 1.00 33.78 C ATOM 1540 O PHE X 203 13.129 57.957 58.876 1.00 33.94 O ATOM 1541 N TYR X 204 11.875 56.599 57.593 1.00 34.96 N ATOM 1542 CA TYR X 204 10.591 57.032 58.124 1.00 36.43 C ATOM 1543 CB TYR X 204 9.480 56.775 57.103 1.00 36.63 C ATOM 1544 CG TYR X 204 8.075 56.885 57.662 1.00 36.91 C ATOM 1545 CD1 TYR X 204 7.522 58.126 57.975 1.00 36.88 C ATOM 1546 CE1 TYR X 204 6.231 58.232 58.487 1.00 37.29 C ATOM 1547 CZ TYR X 204 5.483 57.084 58.685 1.00 37.32 C ATOM 1548 OH TYR X 204 4.210 57.181 59.193 1.00 37.95 O ATOM 1549 CE2 TYR X 204 6.007 55.838 58.378 1.00 37.62 C ATOM 1550 CD2 TYR X 204 7.296 55.745 57.866 1.00 31.39 C ATOM 1551 C TYR X 204 10.312 56.254 59.407 1.00 37.42 C ATOM 1552 O TYR X 204 10.431 55.024 59.434 1.00 37.79 O ATOM 1553 N ARG X 205 9.940 56.968 60.465 1.00 38.43 N ATOM 1554 CA ARG X 205 9.665 56.348 61.767 1.00 39.46 C ATOM 1555 CB ARG X 205 9.767 57.378 62.901 1.00 39.76 C ATOM 1556 CG ARG X 205 11.014 58.268 62.864 1.00 41.74 C ATOM 1557 CD ARG X 205 10.913 59.357 61.791 1.00 44.07 C ATOM 1558 NE ARG X 205 12.076 60.240 61.801 1.00 46.72 N ATOM 1559 CZ ARG X 205 12.019 61.562 61.939 1.00 48.19 C ATOM 1560 NH1 ARG X 205 10.848 62.179 62.064 1.00 48.90 N ATOM 1561 NH2 ARG X 205 13.140 62.274 61.940 1.00 49.01 N ATOM 1562 C ARG X 205 8.295 55.665 61.795 1.00 39.44 C ATOM 1563 O ARG X 205 7.258 56.324 61.900 1.00 39.65 O ATOM 1564 OW0 HOH W 1 17.328 30.841 41.342 1.00 13.55 O ATOM 1565 OW0 HOH W 2 6.080 55.905 47.160 1.00 13.76 O ATOM 1566 OW0 HOH W 3 4.048 59.906 43.347 1.00 14.05 O ATOM 1567 OW0 HOH W 4 0.704 57.513 41.713 1.00 15.24 O ATOM 1568 OW0 HOH W 5 5.651 50.433 35.931 1.00 17.32 O ATOM 1569 OW0 HOH W 6 23.026 64.481 38.188 1.00 10.96 O ATOM 1570 OW0 HOH W 7 12.555 37.870 34.182 1.00 19.82 O ATOM 1571 OW0 HOH W 8 11.922 54.852 55.497 1.00 18.27 O ATOM 1572 OW0 HOH W 9 8.818 43.207 50.381 1.00 14.96 O ATOM 1573 OW0 HOH W 10 27.800 66.142 44.438 1.00 26.20 O ATOM 1574 OW0 HOH W 11 11.787 57.813 54.420 1.00 19.92 O ATOM 1575 OW0 HOH W 12 3.596 40.091 46.527 1.00 22.31 O ATOM 1576 OW0 HOH W 13 29.206 53.589 53.969 1.00 21.19 O ATOM 1577 OW0 HOH W 14 3.076 67.750 41.467 1.00 22.33 O ATOM 1578 OW0 HOH W 15 26.620 64.484 38.091 1.00 17.05 O ATOM 1579 OW0 HOH W 16 19.359 34.768 41.035 1.00 15.57 O ATOM 1580 OW0 HOH W 17 21.010 64.857 40.624 1.00 16.67 O ATOM 1581 OW0 HOH W 18 19.888 36.931 39.432 1.00 23.52 O ATOM 1582 OW0 HOH W 19 27.622 58.662 34.483 1.00 19.48 O ATOM 1583 OW0 HOH W 20 12.579 47.128 55.855 1.00 16.64 O ATOM 1584 OW0 HOH W 21 5.023 62.006 44.777 1.00 17.04 O ATOM 1585 OW0 HOH W 22 12.419 69.310 25.291 1.00 22.84 O ATOM 1586 OW0 HOH W 23 18.045 48.671 59.395 1.00 34.19 O ATOM 1587 OW0 HOH W 24 21.204 72.606 40.012 1.00 19.76 O ATOM 1588 OW0 HOH W 25 8.025 55.157 31.622 1.00 19.49 O ATOM 1589 OW0 HOH W 26 21.198 59.382 32.295 1.00 16.12 O ATOM 1590 OW0 HOH W 27 26.059 47.843 37.230 1.00 20.06 O ATOM 1591 OW0 HOH W 28 23.148 42.497 59.773 1.00 20.86 O ATOM 1592 OW0 HOH W 29 15.204 68.185 50.111 1.00 23.32 O ATOM 1593 OW0 HOH W 30 13.652 67.881 37.933 1.00 24.96 O ATOM 1594 OW0 HOH W 31 7.882 66.774 31.839 1.00 28.46 O ATOM 1595 OW0 HOH W 32 20.646 71.206 42.370 1.00 16.13 O ATOM 1596 OW0 HOH W 33 28.643 37.966 50.225 1.00 34.91 O ATOM 1597 OW0 HOH W 34 9.845 56.350 30.277 1.00 20.05 O ATOM 1598 OW0 HOH W 35 28.649 40.248 55.059 1.00 23.37 O ATOM 1599 OW0 HOH W 36 8.995 59.883 55.595 1.00 35.28 O ATOM 1600 OW0 HOH W 37 9.497 44.727 54.972 1.00 27.65 O ATOM 1601 OW0 HOH W 38 3.544 51.694 37.348 1.00 19.23 O ATOM 1602 OW0 HOH W 39 8.560 64.565 24.883 1.00 36.70 O ATOM 1603 OW0 HOH W 40 7.634 78.032 23.865 1.00 79.88 O ATOM 1604 OW0 HOH W 41 28.072 34.958 45.439 1.00 29.28 O ATOM 1605 OW0 HOH W 42 5.695 48.163 44.053 1.00 23.93 O ATOM 1606 OW0 HOH W 43 3.718 38.814 35.388 1.00 28.41 O ATOM 1607 OW0 HOH W 44 7.063 67.338 24.804 0.50 44.82 O ATOM 1608 OW0 HOH W 45 23.810 53.672 33.588 1.00 20.53 O ATOM 1609 OW0 HOH W 46 25.722 66.756 37.670 1.00 21.69 O ATOM 1610 OW0 HOH W 47 22.474 43.283 37.008 1.00 31.52 O ATOM 1611 OW0 HOH W 48 1.425 57.265 52.722 1.00 36.34 O ATOM 1612 OW0 HOH W 49 11.169 64.598 28.922 1.00 28.96 O ATOM 1613 OW0 HOH W 50 34.865 61.830 51.709 1.00 29.19 O ATOM 1614 OW0 HOH W 51 20.529 48.485 57.562 1.00 24.13 O ATOM 1615 OW0 HOH W 52 24.623 55.853 32.317 1.00 20.99 O ATOM 1616 OW0 HOH W 53 18.317 47.805 33.566 1.00 28.59 O ATOM 1617 OW0 HOH W 54 12.895 50.563 28.740 1.00 28.50 O ATOM 1618 OW0 HOH W 55 −6.406 62.362 36.578 1.00 29.56 O ATOM 1619 OW0 HOH W 56 9.486 71.154 31.652 1.00 30.54 O ATOM 1620 OW0 HOH W 57 4.077 66.859 46.968 1.00 23.74 O ATOM 1621 OW0 HOH W 58 6.314 42.630 49.426 1.00 38.02 O ATOM 1622 OW0 HOH W 59 7.069 33.565 57.902 1.00 33.17 O ATOM 1623 OW0 HOH W 60 10.022 65.589 31.172 1.00 25.32 O ATOM 1624 OW0 HOH W 61 −4.508 58.652 36.641 1.00 25.95 O ATOM 1625 OW0 HOH W 62 1.246 50.368 36.771 1.00 23.12 O ATOM 1626 OW0 HOH W 63 18.779 58.847 31.608 1.00 22.18 O ATOM 1627 OW0 HOH W 64 10.843 63.364 23.031 1.00 32.27 O ATOM 1628 OW0 HOH W 65 20.715 62.939 42.136 1.00 29.55 O ATOM 1629 OW0 HOH W 66 0.328 69.253 45.663 1.00 51.19 O ATOM 1630 OW0 HOH W 67 27.567 64.900 34.385 1.00 28.74 O ATOM 1631 OW0 HOH W 68 39.584 55.441 52.982 1.00 56.79 O ATOM 1632 OW0 HOH W 69 22.538 62.716 53.219 1.00 31.08 O ATOM 1633 OW0 HOH W 70 2.632 68.395 43.791 1.00 45.23 O ATOM 1634 OW0 HOH W 71 13.947 35.361 33.766 1.00 28.13 O ATOM 1635 OW0 HOH W 72 35.236 59.612 45.072 1.00 56.78 O ATOM 1636 OW0 HOH W 73 0.229 49.559 39.212 1.00 30.20 O ATOM 1637 OW0 HOH W 74 0.068 61.386 44.549 1.00 33.50 O ATOM 1638 OW0 HOH W 75 20.812 70.177 47.212 1.00 29.48 O ATOM 1639 OW0 HOH W 76 33.222 41.166 46.547 1.00 102.49 O ATOM 1640 OW0 HOH W 77 32.816 57.977 51.761 1.00 40.60 O ATOM 1641 OW0 HOH W 78 6.562 43.845 33.931 1.00 35.81 O ATOM 1642 OW0 HOH W 79 18.456 56.610 32.498 1.00 28.33 O ATOM 1643 OW0 HOH W 80 27.538 69.465 35.257 1.00 32.01 O ATOM 1644 OW0 HOH W 81 26.080 52.220 32.438 1.00 41.15 O ATOM 1645 OW0 HOH W 82 27.106 47.009 58.301 1.00 34.89 O ATOM 1646 OW0 HOH W 83 23.231 64.872 28.117 1.00 31.34 O ATOM 1647 OW0 HOH W 84 24.934 64.631 52.680 1.00 31.24 O ATOM 1648 OW0 HOH W 85 3.258 42.665 38.698 1.00 31.19 O ATOM 1649 OW0 HOH W 86 18.392 36.517 33.372 1.00 30.32 O ATOM 1650 OW0 HOH W 87 24.722 46.488 59.681 1.00 35.45 O ATOM 1651 OW0 HOH W 88 16.606 65.079 28.152 1.00 29.22 O ATOM 1652 OW0 HOH W 89 8.072 43.179 52.938 1.00 33.09 O ATOM 1653 OW0 HOH W 90 −1.402 61.539 41.769 1.00 34.63 O ATOM 1654 OW0 HOH W 91 34.094 43.537 51.724 1.00 38.99 O ATOM 1655 OW0 HOH W 92 17.093 54.167 30.473 1.00 36.50 O ATOM 1656 OW0 HOH W 93 8.340 67.613 47.745 1.00 28.60 O ATOM 1657 OW0 HOH W 94 6.149 52.606 56.112 1.00 37.53 O ATOM 1658 OW0 HOH W 95 2.948 40.454 40.144 1.00 30.02 O ATOM 1659 OW0 HOH W 96 28.110 49.723 54.553 1.00 38.42 O ATOM 1660 OW0 HOH W 97 22.675 57.203 31.092 1.00 29.09 O ATOM 1661 OW0 HOH W 98 30.410 50.169 49.746 1.00 35.38 O ATOM 1662 OW0 HOH W 99 20.420 54.974 31.004 1.00 36.31 O ATOM 1663 OW0 HOH W 100 −4.624 67.662 37.763 1.00 29.29 O ATOM 1664 OW0 HOH W 101 32.903 59.925 53.842 1.00 57.65 O ATOM 1665 OW0 HOH W 102 8.251 48.921 57.957 1.00 32.56 O ATOM 1666 OW0 HOH W 103 9.529 69.708 43.742 1.00 34.52 O ATOM 1667 OW0 HOH W 104 −1.125 51.287 33.096 1.00 51.90 O ATOM 1668 OW0 HOH W 105 22.008 61.060 30.191 1.00 33.62 O ATOM 1669 OW0 HOH W 106 7.453 59.198 61.616 1.00 70.12 O ATOM 1670 OW0 HOH W 107 −0.874 51.284 40.694 1.00 35.22 O ATOM 1671 OW0 HOH W 108 20.943 41.645 35.526 1.00 32.26 O ATOM 1672 OW0 HOH W 109 5.620 70.636 25.877 1.00 61.44 O ATOM 1673 OW0 HOH W 110 22.275 54.122 60.734 1.00 36.32 O ATOM 1674 OW0 HOH W 111 26.307 66.084 49.785 1.00 34.11 O ATOM 1675 OW0 HOH W 112 11.407 48.301 58.411 1.00 29.02 O ATOM 1676 OW0 HOH W 113 26.806 63.460 31.122 1.00 34.71 O ATOM 1677 OW0 HOH W 114 8.884 30.582 40.992 1.00 36.30 O ATOM 1678 OW0 HOH W 115 11.479 63.554 59.426 1.00 56.37 O ATOM 1679 OW0 HOH W 116 14.803 63.347 29.162 1.00 39.68 O ATOM 1680 OW0 HOH W 117 12.150 53.474 60.742 1.00 41.19 O ATOM 1681 OW0 HOH W 118 28.575 47.112 48.173 1.00 34.99 O ATOM 1682 OW0 HOH W 119 10.076 30.133 34.488 1.00 47.15 O ATOM 1683 OW0 HOH W 120 28.402 61.033 31.016 1.00 39.73 O ATOM 1684 OW0 HOH W 121 25.479 67.945 46.049 1.00 39.36 O ATOM 1685 OW0 HOH W 122 0.734 56.578 47.909 1.00 38.00 O ATOM 1686 OW0 HOH W 123 9.395 49.839 30.508 1.00 35.16 O ATOM 1687 OW0 HOH W 124 31.550 49.603 47.391 1.00 32.71 O ATOM 1688 OW0 HOH W 125 0.816 41.368 43.751 1.00 35.22 O ATOM 1689 OW0 HOH W 126 5.486 47.377 47.613 1.00 44.89 O ATOM 1690 OW0 HOH W 127 31.113 51.669 45.682 1.00 38.04 O ATOM 1691 OW0 HOH W 128 1.436 59.923 42.862 1.00 26.88 O ATOM 1692 OW0 HOH W 129 18.586 43.366 33.846 1.00 38.47 O ATOM 1693 OW0 HOH W 130 16.561 69.834 48.474 1.00 32.88 O ATOM 1694 OW0 HOH W 131 34.295 43.982 47.100 1.00 68.28 O ATOM 1695 OW0 HOH W 132 5.298 34.689 38.229 1.00 49.39 O ATOM 1696 OW0 HOH W 133 3.300 35.280 40.898 1.00 35.60 O ATOM 1697 OW0 HOH W 134 12.437 70.145 37.142 1.00 40.97 O ATOM 1698 OW0 HOH W 135 27.766 65.255 29.010 1.00 36.38 O ATOM 1699 OW0 HOH W 136 6.084 41.962 46.625 1.00 47.13 O ATOM 1700 OW0 HOH W 137 5.960 49.922 55.359 1.00 34.78 O ATOM 1701 OW0 HOH W 138 19.695 36.565 36.333 1.00 37.61 O ATOM 1702 OW0 HOH W 139 26.815 55.165 58.919 1.00 61.16 O ATOM 1703 OW0 HOH W 140 21.895 52.796 31.863 1.00 42.17 O ATOM 1704 OW0 HOH W 141 1.460 65.803 50.346 1.00 40.26 O ATOM 1705 OW0 HOH W 142 4.323 46.032 44.857 1.00 37.04 O ATOM 1706 OW0 HOH W 143 13.181 47.109 33.427 1.00 40.11 O ATOM 1707 OW0 HOH W 144 18.021 60.476 62.244 1.00 58.10 O ATOM 1708 OW0 HOH W 145 34.229 42.239 57.157 1.00 45.39 O ATOM 1709 OW0 HOH W 146 22.591 48.081 59.775 1.00 32.69 O ATOM 1710 OW0 HOH W 147 7.290 34.657 36.557 1.00 38.37 O ATOM 1711 OW0 HOH W 148 30.205 51.763 38.560 1.00 43.21 O ATOM 1712 OW0 HOH W 149 9.512 60.561 58.672 1.00 47.60 O ATOM 1713 OW0 HOH W 150 1.012 47.871 35.078 1.00 33.28 O ATOM 1714 OW0 HOH W 151 28.668 53.537 56.609 1.00 43.90 O ATOM 1715 OW0 HOH W 152 29.440 57.784 36.320 1.00 46.19 O ATOM 1716 OW0 HOH W 153 14.716 41.521 57.984 1.00 42.10 O ATOM 1717 OW0 HOH W 154 2.405 29.431 51.398 1.00 54.51 O ATOM 1718 OW0 HOH W 155 15.742 47.273 34.380 1.00 32.73 O ATOM 1719 OW0 HOH W 156 −0.872 51.922 36.097 1.00 28.11 O ATOM 1720 OW0 HOH W 157 31.688 65.491 39.530 1.00 39.88 O ATOM 1721 OW0 HOH W 158 21.560 46.873 62.631 1.00 38.78 O ATOM 1722 OW0 HOH W 159 −0.474 56.276 45.289 1.00 46.96 O ATOM 1723 OW0 HOH W 160 6.427 28.246 48.315 1.00 36.54 O ATOM 1724 OW0 HOH W 161 31.532 37.651 51.492 1.00 56.31 O ATOM 1725 OW0 HOH W 162 9.015 29.318 55.310 1.00 42.95 O ATOM 1726 OW0 HOH W 163 28.298 64.095 52.742 1.00 50.08 O ATOM 1727 OW0 HOH W 164 −0.594 60.533 46.930 1.00 41.52 O 

What is claimed is:
 1. A laminin receptor (LamR) crystal comprising residues 1-220 of the full-length human 37 kDa LamR protein, wherein said protein is in the tetragonal spacegroup P4₃2₁2 and has unit cell dimensions of a=75.7 Angstroms, b=75.7 Angstroms and c=99.0 Angstroms.
 2. The LamR crystal of claim 1 having atomic structure coordinates listed in Appendix 1, or any part thereof.
 3. The LamR crystal of claim 1 having an active site including relative structural coordinates of amino acid residues Leu25, Gly26, Gly27 Ile46, Arg80, Asp126, Arg128, Asn149, Asp151, Ser152, Asp164, Asp165, Lys166, Gln33, Gln35, Tyr36, Lys57, Ala137, Ser138, Asn141, Leu142, Pro143, Thr144, Met10, Leu19, His24, Ile49, Asn50, Leu51, Lys52, Gln9, Lys11, Trp55, Leu59, Glu181, Arg184, Met185 and Arg191, or any portion thereof, and having a root mean square deviation from residue backbone atoms of said amino acid residues of not more than 1.5 Å.
 4. The LamR crystal of claim 3, wherein said active site is complexed with a compound, said active site includes, relative structural coordinates of amino acid residues: Leu25, Gly26, Gly27, Thr28, Ile46, Asp126, Pro127, Arg128, Ala129, Asn149, Asp151, Ser152, Asn164, Asn165, Lys166, Val15, Phe18, Leu19, His24, Leu25, Ile49, Asn50, Leu51, and Lys52, Arg80 and Asn81, or any portion thereof, and a root mean square deviation from residue backbone atoms of said amino acid residues being not more than 1.5 Å.
 5. The LamR crystal of claim 3, wherein said active site is complexed with a protein or peptide, said active site includes relative structural coordinates of amino acid residues: Gln33, Leu60, Arg63, Ala64, Ala67, Asn70, Pro71, Ala72, Asp73, Val74, Ser75, Ala91, Ala92, Ala93, Thr94, Gly95, Ala96, Thr97, Pro98, Ile99, Arg102, Phe103, Thr104, Pro105, Gly106, Thr107, Phe108, Thr109, Asn110, Gln111, Ile112, Gln113, Ala114, Ala115, Phe116, Arg117, Glu118, Pro119, Arg120, Leu121, Leu134, Ala137, Ser138, Asn141, Leu142, Pro143, Thr144, Ile145, Leu154, Arg155, Tyr156, Val157, Asp158, Ile159, Leu183, Arg186, or any portion thereof, and a root mean square deviation from residue backbone atoms of said amino acid residues being not more than 1.5 Å.
 6. The LamR crystal of claim 3, wherein said active site is complexed with a protein or peptide, said active site includes relative structural coordinates of amino acid, residues: Ala72, Asp73, Val74, Ser75, Thr97, Ile99, Thr104, Pro105, Gly106, Thr107, Phe108, Asn110, Gln111, Ile112, Gln113, Ala114, Ala115, Phe116, Arg117, Glu118, Pro119, or any portion thereof, and a root mean square deviation from residue backbone atoms of said amino acid residues being not more than 1.5 Å. 